Fingerprint spectrum detection method for meridian warming decoction

A fingerprint and detection method technology, which is applied in the detection field of medicinal preparations, can solve problems such as inability to reflect the integrity characteristics of traditional Chinese medicine compound preparations and incomplete drug quality

Active Publication Date: 2020-03-24
SHANDONG WOHUA PHARMACEUTICALS CO LTD
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Problems solved by technology

Existing literature only records the content determination of several components of Wenjing Decoction by HPLC method, but it is obviously not comprehensive to control the quality of it

Method used

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  • Fingerprint spectrum detection method for meridian warming decoction
  • Fingerprint spectrum detection method for meridian warming decoction
  • Fingerprint spectrum detection method for meridian warming decoction

Examples

Experimental program
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[0095] Example 1

[0096] 1. Preparation of standard decoction mixed reference solution of Wenjing Decoction: accurately weigh ferulic acid, paeoniflorin, cinnamaldehyde, paeonol, gemmazone, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, glycyrrhizin , Ammonium glycyrrhizinate, β-ecdysterone reference substance, add methanol to make a mixed reference substance with mass concentrations of 129.5, 89.2, 321.4, 58.7, 232.6, 117.9, 235.6, 124.8, 136.2, 259.3, 285.6 μg / mL Solution

[0097] 2. Preparation of standard decoction test solution of Wenjing Decoction: Weigh 1.67g each of Angelica sinensis, Ligusticum chuanxiong, Radix Paeoniae Alba, cinnamon, Cortex Moutan, and Zedoary Turmeric powder, and 3.33g each of ginseng, licorice and Achyranthes bidentata powder, add 300mL of water, and mix Evenly, first heat to boiling with martial fire, then switch to slow decoction, wait until the decoction liquid reaches about 160mL, filter while hot, add water to the filtrate to make the volume...

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Abstract

The invention relates to a fingerprint spectrum detection method for meridian warming decoction. The method comprises the following steps: 1) preparing a test solution; weighing 1-2g of angelica sinensis, 1-2g of ligusticum wallichii, 1-2g of radix paeoniae alba, 1-2g of cinnamon, 1-2g of moutan bark, 1-2g of curcuma zedoary powder, weighing 3-4g of ginseng powder, 3-4g of liquorice powder and 3-4g of radix achyranthis bidentatae powder; adding 250-350mL of water, uniformly mixing, heating to boil with strong fire, slowly decocting with slow fire, filtering while the decoction is about 150-170mL, and adding water into the filtrate to dilute to 240-260mL; precisely sucking 8-12mL of the standard decoction of the meridian warming decoction, adding methanol to dilute to 40-60mL, sealing, weighing the mass, carrying out ultrasonic (the power is 180-220W and the frequency is 40-60kHz) treatment for 8-12min, standing overnight, weighing the mass again, supplementing the lost mass with methanol, uniformly shaking, filtering, and taking the subsequent filtrate, thereby obtaining the test solution; 2) detecting: taking and injecting 5-15 [mu]L of the test solution obtained in the previous step into a high performance liquid chromatograph, and obtaining a chromatogram; and 3) performing result judgment: comparing the chromatogram obtained in the previous step with a standard control fingerprint, and if the similarity is greater than 90%, the sample being qualified.

Description

technical field [0001] The invention relates to a detection method of a medicinal preparation, in particular to a detection method of a traditional Chinese medicine Wenjing Decoction fingerprint. Background technique [0002] Irregular menstruation, dysmenorrhea, and metrorrhagia are common gynecological diseases, which not only bring great pain to patients, but also burden families and society. Wenjing Decoction is a classic prescription for regulating menstruation and relieving pain in traditional Chinese medicine. It comes from "Women's Encyclopedia of Good Prescriptions" written by Chen Ziming, a famous doctor in the Southern Song Dynasty. It is now included in the "Catalog of Ancient Classical Prescriptions". Composed of curcuma, ginseng, licorice, and achyranthes bidentata, it has the effects of warming the meridian and dispelling cold, promoting blood circulation and regulating menstruation. It is mainly used for late menstruation or too little, amenorrhea, and dysmen...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/047
Inventor 王炯程世娟周万辉曾英姿王冬梅赵磊
Owner SHANDONG WOHUA PHARMACEUTICALS CO LTD
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