Fingerprint spectrum detection method for meridian warming decoction

Abstract

The invention relates to a fingerprint spectrum detection method for meridian warming decoction. The method comprises the following steps: 1) preparing a test solution; weighing 1-2g of angelica sinensis, 1-2g of ligusticum wallichii, 1-2g of radix paeoniae alba, 1-2g of cinnamon, 1-2g of moutan bark, 1-2g of curcuma zedoary powder, weighing 3-4g of ginseng powder, 3-4g of liquorice powder and 3-4g of radix achyranthis bidentatae powder; adding 250-350mL of water, uniformly mixing, heating to boil with strong fire, slowly decocting with slow fire, filtering while the decoction is about 150-170mL, and adding water into the filtrate to dilute to 240-260mL; precisely sucking 8-12mL of the standard decoction of the meridian warming decoction, adding methanol to dilute to 40-60mL, sealing, weighing the mass, carrying out ultrasonic (the power is 180-220W and the frequency is 40-60kHz) treatment for 8-12min, standing overnight, weighing the mass again, supplementing the lost mass with methanol, uniformly shaking, filtering, and taking the subsequent filtrate, thereby obtaining the test solution; 2) detecting: taking and injecting 5-15 [mu]L of the test solution obtained in the previous step into a high performance liquid chromatograph, and obtaining a chromatogram; and 3) performing result judgment: comparing the chromatogram obtained in the previous step with a standard control fingerprint, and if the similarity is greater than 90%, the sample being qualified.

Description

technical field [0001] The invention relates to a detection method of a medicinal preparation, in particular to a detection method of a traditional Chinese medicine Wenjing Decoction fingerprint. Background technique [0002] Irregular menstruation, dysmenorrhea, and metrorrhagia are common gynecological diseases, which not only bring great pain to patients, but also burden families and society. Wenjing Decoction is a classic prescription for regulating menstruation and relieving pain in traditional Chinese medicine. It comes from "Women's Encyclopedia of Good Prescriptions" written by Chen Ziming, a famous doctor in the Southern Song Dynasty. It is now included in the "Catalog of Ancient Classical Prescriptions". Composed of curcuma, ginseng, licorice, and achyranthes bidentata, it has the effects of warming the meridian and dispelling cold, promoting blood circulation and regulating menstruation. It is mainly used for late menstruation or too little, amenorrhea, and dysmen...

AI Technical Summary

Problems solved by technology

Existing literature only records the content determination of several components of Wenjing Decoction by HPLC method, but it is obviously not comprehensive to control the quality of its medicines simply by detecting the quantification of several index components, nor can it reflect the overall quality of Chinese medicine compound preparations. sexual characteristics

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