The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of
traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of
bergenin,
arbutin, glycyrrhizic acid,
liquiritin,
liquiritigenin and
gallic acid in a
solvent to obtain reference substance solutions; adding soroxil decoction into a
solvent, carrying out ultrasonic extraction, and filtering to obtain a
test solution; and injecting the reference solution and the
test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the
test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction,
rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in
quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.