53results about How to "Many peaks" patented technology

The invention relates to a fingerprint spectrum detection method for meridian warming decoction. The method comprises the following steps: 1) preparing a test solution; weighing 1-2g of angelica sinensis, 1-2g of ligusticum wallichii, 1-2g of radix paeoniae alba, 1-2g of cinnamon, 1-2g of moutan bark, 1-2g of curcuma zedoary powder, weighing 3-4g of ginseng powder, 3-4g of liquorice powder and 3-4g of radix achyranthis bidentatae powder; adding 250-350mL of water, uniformly mixing, heating to boil with strong fire, slowly decocting with slow fire, filtering while the decoction is about 150-170mL, and adding water into the filtrate to dilute to 240-260mL; precisely sucking 8-12mL of the standard decoction of the meridian warming decoction, adding methanol to dilute to 40-60mL, sealing, weighing the mass, carrying out ultrasonic (the power is 180-220W and the frequency is 40-60kHz) treatment for 8-12min, standing overnight, weighing the mass again, supplementing the lost mass with methanol, uniformly shaking, filtering, and taking the subsequent filtrate, thereby obtaining the test solution; 2) detecting: taking and injecting 5-15 [mu]L of the test solution obtained in the previous step into a high performance liquid chromatograph, and obtaining a chromatogram; and 3) performing result judgment: comparing the chromatogram obtained in the previous step with a standard control fingerprint, and if the similarity is greater than 90%, the sample being qualified.

The invention discloses a preparation method of a natural high-concentration black tea essence. The method comprises the following steps: (1) tea leaves are added into a sealed extraction tank with a steam heating and condensing device; steam is delivered in for distillation; a condensate is collected, such that black tea distillate liquid A is obtained; (2) the liquid A is collected, and when the weight of the liquid A reaches 2-6 times that the weight of the raw material (tea leaves), collection is stopped, and the liquid A is refrigerated; (3) the obtained liquid A is extracted with an SCC (spinning cone column) method; and through adjusting extraction parameters, the natural high-concentration black tea essence B is obtained. According to the invention, distillation extraction is carried out with the combination of a steam distillation method and the SCC (spinning cone column) method, such that a series of high-concentration water-soluble black tea essence can be obtained. The product is clear and transparent, and has the advantages of pure and natural aroma and true taste. The product is suitable for beverages, dairy products, and ice products. The product is an upgrade and replacement of traditional food flavors. The materials are natural, and the process is advanced, such that current consumption demands of naturalness and healthiness can be satisfied.

The invention discloses a HPLC fingerprint detection method of a sanajon oral solution. The fingerprint has the advantages of more appearance, good resolution, good repeatability and high precision, and provides effective guarantee to comprehensive monitor on the quality of a sanajon oral solution. The extraction method and chromatographic condition of the fingerprint detection method are utilizedto detect content of five active ingredients in the sanajon oral solution, including gallic acid, allocatechine, picatechin, corilagin and ellagic acid, and realizes qualitative and quantitative researches on the sanajon oral solution.

The invention provides a method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotate. In the detection method, the peak amount is large, separation degree is high, a base line is stable, the lamiophlomis rotate can be effectively detected, and new selection is provided for guaranteeing of medicine material quality. The iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients are detected under the same chromatographic condition for the first time, seven specific compounds are identified, and the quality of lamiophlomis rotate medicine materials can be comprehensively and accurately controlled.

The invention discloses a raw fleece-flower root HPLC fingerprint detection method. The detection method comprises the following steps: (1) preparing a control substance solution; (2) preparing a reference substance solution; (3) establishing a control fingerprint; (4) preparing a test article solution; (5) detecting the test article solution. The obtained fingerprint has the advantages that there are many peaks, the resolution and repeatability are good, the precision is high, and the quality of raw fleece-flower root can be effectively monitored. By using the extraction method and chromatogram conditions of provided fingerprint detection method, contents of 16 components of raw fleece-flower root can be measured, and thus the quality of raw fleece-flower root can be monitored from more aspects.

A processing chain for a digital image signal (12) applies a dither pattern (14), having a first spectrum, to the image signal at a point in the processing chain. A further noise pattern (10) is applied to the image signal during the processing chain. The noise pattern (10) has a second spectrum which is configured such that the combination of the first spectrum and second spectrum results in a more continuous spectrum. Another aspect describes a noise pattern (10) which can be used as an offset dither pattern for digital images, especially before colour bit depth reduction. The noise pattern comprises an array of values which are linearly distributed across a range, with each value in the range occurring an equal number of times. Similar values at extreme ends of the range of values are dispersed within the array. The pattern has a Poisson-disk two-dimensional spectral energy distribution. Values are positioned in the array based on distance to similar values in neighbouring repetitions of the array. The array has “magic square” properties.

The invention relates to a determination method of a capsule fingerprint spectrum for dredging collaterals and reducing phlegm, which comprises the following steps: (1) test solution prepartion: taking capsule contents for dredging collaterals and reducing phlegm, adding methanol, carrying out ultrasonic extraction, filtering, and taking the subsequent filtrate to obtain the test solution; (2) preparation of reference substance solutions: taking tauroursodeoxycholic acid, gastrodin, sodium tanshinol, tanshinone IIA, salvianolic acid B, protocatechuic aldehyde, ginsenoside Rb1, ginsenoside Rg1,notoginsenoside R1, rheum emodin and rheinic acid reference substances which are dried under reduced pressure to constant weight, and respectively adding methanol to prepare the reference substance solutions; (3) determination: precisely measuring the test solution and the reference solution respectively, injecting the solutions into a high performance liquid chromatograph for determination, andrecording chromatograms; and (4) performing comparison according to the chromatogram and the standard comparison fingerprint, and determining that the product is qualified if the chromatogram and thestandard comparison fingerprint are consistent.

The invention provides a non-reducing peptide mapping method of a protein. The method comprises the following steps: denaturing the protein by using a denaturing agent, carrying out enzymolysis, terminating the reaction with an acid, and performing detection by reversed phase high-performance liquid chromatography. The non-reducing peptide mapping method makes enzymolysis of the protein sufficient, realizes uniform distribution, large number and good resolution of chromatographic peaks in a map obtained by the reversed phase high-performance liquid chromatography detection, allows the chromatogram to have a stable baseline and truly reflect the situation of each peptide fragment obtained after the enzymolysis of the protein, and is of great significance to controlling the quality of the protein.

The invention discloses a loquat lung-heat-clearing drink reference extract, a preparation method and a quality control method, and belongs to the technical field of traditional Chinese medicine detection.The loquat lung-heat-clearing drink reference extract freeze-dried powder stable in quality is obtained by controlling technological parameters of medicinal material pretreatment, decoction, concentration, freeze drying and the like of a loquat lung-heat-clearing drink prescription. The freeze-dried powder is used for quality research of the loquat lung-heat-clearing drink, the quality is uniform and stable, the detection result is reliable, the specific chromatogram and the identification method are suitable for qualitative detection of various dosage forms of the loquat lung-heat-clearing drink, the related specific chromatogram construction method discloses a detection method of a reference extract of the loquat lung-heat-clearing drink with raw materials easy to obtain, and the method is suitable for large-scale popularization and application. The detection method is simple, convenient and reliable, and provides powerful guarantee for qualitative identification of the loquat lung-heat-clearing drink and the preparation thereof.

The invention provides a method for constructing the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of rhododendron mariae. The method comprises the following steps of: (1) test solution predation: the leaves and twigs of the rhododendron mariae are crushed and weighed, an obtained substance is arranged in an extractor, petroleum ether is added to the extractor, heat reflux extraction is carried out, and the petroleum ether is discarded, and methanol is added, heating reflux is carried out, methanol is supplemented, and filtering is carried out; (2) reference substance solution preparation: rutin, quercitrin, hyperoside, quercetin, kaempferol, an oleanolic acid reference substance are selected, and a mixed reference substance solution is prepared with methanol adopted as a solvent; and (3) and measurement: the reference substance solution and the test solution are injected into a liquid chromatograph separately, and chromatograms are recorded in 105 minutes, and the chromatograms are processed with fingerprint spectrum software, so that the fingerprint spectrum of the leaves and twigs of the rhododendron mariae can be obtained. With the method for constructing the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of the rhododendron mariae of the invention adopted, the common model of the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of the rhododendron mariae is established, and 16 common peaks are calibrated, so that the quality of the medicinal leaves and twigs of the rhododendron mariae can be effectively characterized; the singleness and one-sidedness of an original quality control method are avoided. The method has a relatively high application value.

The invention belongs to the field of detection, and provides a method for determining chemical components in paeonia lactiflora and liquorice decoction and a method for establishing a fingerprint spectrum, the method comprises the following steps: mixing a paeonia lactiflora and liquorice decoction sample with a solvent, and dissolving to obtain a to-be-detected solution; the method comprises the following steps: weighing a reference substance, respectively obtaining chromatograms of chemical components in a paeonia lactiflora and liquorice decoction solution and the reference substance under the same detection conditions by adopting a high performance liquid chromatography, and determining the content of the paeonia lactiflora and liquorice decoction according to the concentration of the reference substance, the peak area of the reference substance in the chromatograms and the peak area of components corresponding to the reference substance in the paeonia lactiflora and liquorice decoction in the chromatograms. And calculating the contents of active ingredients of paeoniflorin, liquiritin and ammonium glycyrrhizinate in the Chinese herbaceous peony and liquorice decoction by an external standard two-point method based on a dual-wavelength detection condition. According to the method, under the same chromatographic condition, the chemical components in the paeonia lactiflora and liquorice decoction and the specific content of the corresponding chemical components can be simultaneously identified and detected only by adjusting the wavelength, the result is more accurate and reliable, and good reproducibility and stability are achieved.

The invention provides a construction method of a lindley eupatorium herb formula granule fingerprint spectrum, a standard fingerprint spectrum of the lindley eupatorium herb formula granule and application of the standard fingerprint spectrum, and belongs to the technical field of medicine detection.The construction method comprises the steps that a test solution, a reference medicinal material solution and a reference substance solution are prepared, acetonitrile (A) and a 0.1-0.3 wt% phosphoric acid aqueous solution (B) are jointly used as mobile phases, and the standard fingerprint spectrum of the lindley eupatorium herb formula granule is obtained. Performing high performance liquid chromatography detection to obtain a fingerprint spectrum of the lindley eupatorium herb formula granules; the standard fingerprint spectrum of the lindley eupatorium herb formula granules is obtained by using the construction method, and the standard fingerprint spectrum is used for quality evaluation or control in the whole process of research/development/production/clinical application of the lindley eupatorium herb formula granules. According to the method for constructing the fingerprint spectrum of the lindley eupatorium herb formula granules, main medicinal material components in the lindley eupatorium herb formula granules can be detected only through high performance liquid chromatography, and the quality condition of the lindley eupatorium herb formula granules can be comprehensively reflected.