HPLC fingerprint identification method for origin ginseng protection
A fingerprint and origin technology, applied in the field of HPLC fingerprint identification, achieves the effects of good stability, good separation and simple pretreatment method
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[0052] 1. Establish the liquid chromatography fingerprint of ginseng in the place of origin
[0053] A. Establish the liquid chromatography fingerprint of ginseng in the place of origin
[0054] 1. Preparation of reference solution
[0055] Accurately weigh an appropriate amount of ginsenoside Re reference substance, add methanol to dissolve and make a 1.00 mg / mL reference substance solution.
[0056] 2. Preparation of standard solution
[0057] Put commercially available Changbai Mountain ginseng as a standard product in a drying oven at 60°C for 4 hours and heat to constant weight, crush and sieve, and collect powder samples below 80 mesh;
[0058] Accurately weigh 2.0g Changbai Mountain ginseng powder sample and add it to the automatic temperature-controlled solvent reflux liquid-solid extractor, add 100mL methanol for extraction for 5 hours, extract the solution by rotary evaporation to recover the solvent, and pour the concentrated solution into a 10mL volumetric flask;...
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