Method for detecting tyrosine by using chitosan-functionalized gold nano-modified glassy carbon electrode

A technology of glassy carbon electrode and chitosan, which is applied in the field of detection of tyrosine, can solve the problems of high detection limit that cannot meet rapid detection, insensitive detection of mixed samples, and large consumption of reagents, so as to eliminate electronic interference factors, electronic The effect of fast transfer rate and simple preprocessing method

Active Publication Date: 2017-10-20
美也环境科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods for determining tyrosine mainly include amino acid automatic analyzer, spectrophotometry, high-performance liquid chromatography, mass spectrometry, nuclear magnetic resonance spectroscopy and capillary electrophoresis, etc. Although these techniques are widely used, the instruments used are expensive and advanced. The processing is complex, time-consuming, consumes a lot of reagents and takes a long time to analyze, mixed samples are not sensitive to detection, and the high detection limit cannot meet the needs of on-site rapid detection

Method used

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  • Method for detecting tyrosine by using chitosan-functionalized gold nano-modified glassy carbon electrode
  • Method for detecting tyrosine by using chitosan-functionalized gold nano-modified glassy carbon electrode

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Embodiment 1

[0037] A gold nano-modified glassy carbon electrode functionalized with chitosan, the preparation method comprising the following steps:

[0038] a. Mix hexadecyltrimethylammonium bromide with deionized water, and stir in a water bath at 30°C for 10 minutes, then add 0.01mol / L chloroauric acid solution and stir for 5 minutes, then add a mass volume fraction of 0.455 g / L NaBH 4 Ice water solution, continue to stir for 2 minutes and then stand still for 30 minutes to obtain the seed crystal solution, in which cetyltrimethylammonium bromide, deionized water, chloroauric acid solution and NaBH 4 The mass volume ratio of the ice solution is 0.7g:18ml:0.5ml:0.8ml;

[0039] b. Mix and stir cetyltrimethylammonium bromide, sodium oleate and water for 25 minutes, and after cooling to 25°C, add 5mmol / L AgNO in a water bath at 30°C 3 The solution was stirred for 4 minutes, and after standing for 10 minutes, 0.01 mol / L of chloroauric acid solution was added, stirred until the solution ch...

Embodiment 2

[0043] A gold nano-modified glassy carbon electrode functionalized with chitosan, the preparation method comprising the following steps:

[0044] a. Mix hexadecyltrimethylammonium bromide and deionized water, and stir in a water bath at 35°C for 20 minutes, then add 0.01mol / L chloroauric acid solution and stir for 10 minutes, then add a mass volume fraction of 0.455 g / L NaBH 4 Ice solution, continue to stir for 5 minutes and then stand still for 60 minutes to obtain the seed solution, in which cetyltrimethylammonium bromide, deionized water, chloroauric acid solution and NaBH 4 The mass-to-volume ratio of the ice-water solution is 0.75g:20ml:0.8ml:1.2ml;

[0045] b. Mix and stir cetyltrimethylammonium bromide, sodium oleate and water for 35 minutes, and after cooling to 20°C, add 5mmol / L AgNO in a water bath at 35°C 3 The solution was stirred for 6 minutes, and after standing for 20 minutes, 0.01 mol / L of chloroauric acid solution was added, stirred until the solution change...

Embodiment 3

[0049] A gold nano-modified glassy carbon electrode functionalized with chitosan, the preparation method comprising the following steps:

[0050] a. Mix hexadecyltrimethylammonium bromide with deionized water, and stir in a water bath at 30°C for 15 minutes, then add 0.01mol / L chloroauric acid solution and stir for 8 minutes, then add a mass volume fraction of 0.455 g / L NaBH 4 Ice solution, continue to stir for 3 minutes and then stand still for 35 minutes to obtain the seed crystal solution, in which cetyltrimethylammonium bromide, deionized water, chloroauric acid solution and NaBH 4 The mass volume ratio of the ice solution is 0.73g:18.5ml:0.5ml:1ml;

[0051] b. Mix and stir cetyltrimethylammonium bromide, sodium oleate and water for 30 minutes, and after cooling to 15°C, add 5mmol / L AgNO in a water bath at 30°C 3 The solution was stirred for 5 minutes, and after standing for 15 minutes, 0.01 mol / L of chloroauric acid solution was added, stirred until the solution changed...

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Abstract

The invention discloses a method for detecting tyrosine by using a chitosan-functionalized gold nano-modified glassy carbon electrode. The method comprises the following steps of (1) preparing a mixed liquid A, mixing the mixed liquid and a chitosan solution with the mass fraction of 0.5wt% at the volume ratio of 1:1, carrying out ultrasonic treatment, carrying out centrifugal treatment, taking sediments, dispersing the sediments into water at the mass-volume ratio of (5-8mg):1ml, carrying out ultrasonic treatment to obtain a micro-solution, titrating the micro-solution on the surface of the glassy carbon electrode and airing to obtain the chitosan-functionalized gold nano-modified glassy carbon electrode; and (2) adopting the chitosan-functionalized gold nano-modified glassy carbon electrode prepared from the step (1) as a working electrode, and detecting the concentration of tyrosine in a to-be-detected solution by using a differential pulse voltammetry of a three-electrode system according to a differential pulse voltammetry curve of the tyrosine. The method has the advantages of being simple in operation, fast in detection and high in sensitivity, and high-sensitivity recognition of the tyrosine in the mixed sample solution can be carried out.

Description

technical field [0001] The invention relates to the field of tyrosine concentration detection. More specifically, the present invention relates to a method for detecting tyrosine with a chitosan-functionalized gold nanometer-modified glassy carbon electrode. Background technique [0002] Tyrosine (Tyr) is an aromatic oxyacid, which can be synthesized from phenylalanine in the body, and is an important semi-essential amino acid for the human body. The metabolic disorder of tyrosine can lead to the occurrence of various genetic diseases, such as tyrosinemia, akaponic acid disease, etc., and has a certain correlation with liver and kidney diseases, nervous system degenerative diseases, malignant tumors, etc. . Tyrosine is an indispensable amino acid for the human body. It is the precursor for the synthesis of neurotransmitter catecholamines. Without tyrosine, the human body will have abnormal growth and mental retardation. Therefore, tyrosine in food has generally begun to be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
Inventor 肖琦黄珊冯萌萌吴子华
Owner 美也环境科技(江苏)有限公司
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