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Method for determining concentration of hydrochlorothiazide, losartan and 5-carboxylic acid losartan in plasma by liquid chromatography-mass spectrometry

A technology of hydrochlorothiazide and LC/MS is applied in measurement devices, instruments, scientific instruments, etc., to achieve the effects of simple pretreatment method, fast method and simple operation.

Inactive Publication Date: 2020-07-24
SUZHOU GUOCHEN BIOTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on a method for the simultaneous detection of hydrochlorothiazide, losartan and its active hydroxy acid metabolite losartan 5-carboxylate

Method used

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  • Method for determining concentration of hydrochlorothiazide, losartan and 5-carboxylic acid losartan in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of hydrochlorothiazide, losartan and 5-carboxylic acid losartan in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of hydrochlorothiazide, losartan and 5-carboxylic acid losartan in plasma by liquid chromatography-mass spectrometry

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Experimental materials and instruments

[0032] Hydrochlorothiazide, losartan and its metabolite 5-carboxylate losartan and various deuterated internal standards hydrochlorothiazide-13C6, losartan-d4 and 5-carboxylate losartan-d4 were provided by TRC in Canada. Acetonitrile, methanol, acetic acid, ammonium acetate, formic acid, etc. are all of HPLC grade, from the American Fisher Company, and other chemical reagents are of analytical grade.

[0033] LCMS-8060 mass spectrometer (Shimadzu, Japan), Lab Solutions 6.84 SP1 data acquisition software (Shimadzu, Japan), Mettler XS 105DU electronic balance (Mettler, Switzerland); Eppendorf Centrifuge5424R high-speed cryogenic centrifuge ( Eppendorf, Germany).

[0034] Second, the experimental process

[0035] 1. Preparation of stock solution and working solution

[0036] Weigh an appropriate amount of hydrochlorothiazide, losartan and its metabolite 5-carboxylate losartan and various deuterated internal standards hydrochlo...

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Abstract

The invention provides a method for determining the concentration of hydrochlorothiazide, losartan and active hydroxy acid metabolite 5-carboxylic acid losartan in plasma by liquid chromatography-massspectrometry. The method comprises the following steps of: (1) pretreating a plasma sample; (2) detecting by liquid chromatography-mass spectrometry; (3) preparing a standard curve; and (4) determining the concentration of hydrochlorothiazide, losartan and active hydroxy acid metabolite 5-carboxylic acid losartan in plasma. The method provided by the invention is rapid, accurate, high in sensitivity and simple to operate, and serves clinical application of medicines.

Description

technical field [0001] The invention relates to the technical field of drug analysis, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for measuring the concentrations of hydrochlorothiazide, losartan and their active hydroxy acid metabolite 5-carboxylate losartan in plasma by liquid chromatography-mass spectrometry. Background technique [0002] Losartan Potassium and Hydrochlorothiazide Tablets is a compound preparation containing losartan and hydrochlorothiazide. Losartan is an angiotensin II receptor antagonist and hydrochlorothiazide is a diuretic. It is used in the treatment of hypertension. : Reduces the risk of fatal or non-fatal cardiovascular disease, primary stroke, and myocardial infarction; reduces the risk of stroke in patients with hypertension and left ventricular hypertrophy. There is no literature report on a method for the simultaneous detection of hydrochlorothiazide, losartan and their active hydroxy acid metab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 高书林
Owner SUZHOU GUOCHEN BIOTEK CO LTD
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