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Determination method of chemical components in Chinese herbaceous peony and liquorice decoction and establishment method of fingerprint spectrum

A technology for Shaoyao Gancao Decoction and chemical components, which is applied in the field of determination of chemical components in Shaoyao Gancao Decoction, can solve the problems of unfavorable Shaoyao Gancao Decoction product quality control, poor separation, inaccurate fingerprint detection results, and the like, and achieves good results. Reproducibility and stability, interference removal, accurate results

Pending Publication Date: 2022-03-08
CHENGDU HUASUN GRP INC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Prior art CN103330758 discloses the combination of thin layer chromatography and fingerprint to verify the composition of licorice granules, but its separation is not good, resulting in inaccurate fingerprint detection results, which is not conducive to the quality control of Shaoyao Gancao Decoction

Method used

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  • Determination method of chemical components in Chinese herbaceous peony and liquorice decoction and establishment method of fingerprint spectrum
  • Determination method of chemical components in Chinese herbaceous peony and liquorice decoction and establishment method of fingerprint spectrum
  • Determination method of chemical components in Chinese herbaceous peony and liquorice decoction and establishment method of fingerprint spectrum

Examples

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Effect test

Embodiment 1

[0091] A method for assaying chemical components in Shaoyao Gancao Decoction, comprising the following steps:

[0092] S1. Take 0.2g of Shaoyao Gancao decoction and put it in a 100ml measuring bottle, add 80ml of 70% methanol, and then ultrasonically treat it (power 500W, frequency 40kHz) for 10 minutes to dissolve, take it out, let it cool to normal temperature, and use 70% methanol to the mark. Shake well, filter, and take the filtrate to obtain the test solution;

[0093] S2. Preparation of reference substances and reference substances: take paeoniflorin, liquiritin and ammonium glycyrrhizinate respectively, add 70% methanol to prepare paeoniflorin reference substances containing 100 μg of paeoniflorin per 1 ml, and glycyrrhizin reference substances containing 50 μg of liquiritin per 1 m With the ammonium glycyrrhizinate reference substance containing 50 μg of ammonium glycyrrhizinate in every 1ml, after mixing, obtain the reference substance reference substance, and set as...

Embodiment 2

[0124] The difference from Example 1 is that in step S4, the regression equation of paeoniflorin content is: y=11.445x+13.245;

[0125] The regression equation of the content of liquiritin is: y=34.309x+8.9743;

[0126] The regression equation of glycyrrhizic acid content is: y=6.1194x+2.8247.

[0127] Optionally, the injection concentration of paeoniflorin is 38.70 μg / ml-115.22 μg / ml;

[0128] The injection concentration of the liquiritin is 19.89 μg / ml to 59.20 μg / ml;

[0129] The injection concentration of glycyrrhizic acid is 32.42 μg / ml-95.42 μg / ml.

[0130] In order to prove the accuracy of the regression equation of detection method of the present invention, feature is as follows verification experiment:

[0131] Verification example 6,

[0132] Precisely weigh 26.24mg of paeoniflorin (lot number PCL-#-P857, purity 98.46%), 13.36mg of liquiritin (lot number 111610-201607, purity 93.1%), and 13.30mg of ammonium glycyrrhizinate (lot number 110731-201619, purity 93.0%)...

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Abstract

The invention belongs to the field of detection, and provides a method for determining chemical components in paeonia lactiflora and liquorice decoction and a method for establishing a fingerprint spectrum, the method comprises the following steps: mixing a paeonia lactiflora and liquorice decoction sample with a solvent, and dissolving to obtain a to-be-detected solution; the method comprises the following steps: weighing a reference substance, respectively obtaining chromatograms of chemical components in a paeonia lactiflora and liquorice decoction solution and the reference substance under the same detection conditions by adopting a high performance liquid chromatography, and determining the content of the paeonia lactiflora and liquorice decoction according to the concentration of the reference substance, the peak area of the reference substance in the chromatograms and the peak area of components corresponding to the reference substance in the paeonia lactiflora and liquorice decoction in the chromatograms. And calculating the contents of active ingredients of paeoniflorin, liquiritin and ammonium glycyrrhizinate in the Chinese herbaceous peony and liquorice decoction by an external standard two-point method based on a dual-wavelength detection condition. According to the method, under the same chromatographic condition, the chemical components in the paeonia lactiflora and liquorice decoction and the specific content of the corresponding chemical components can be simultaneously identified and detected only by adjusting the wavelength, the result is more accurate and reliable, and good reproducibility and stability are achieved.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for determining chemical components in Shaoyao Gancao Decoction and a method for establishing fingerprints. Background technique [0002] Shaoyao Gancao Decoction is a one-dose prescription. This prescription mainly treats the syndromes caused by damaged body fluid, insufficient yin and blood, and loss of tendons. In the prescription, peony is sour and cold, nourishes blood and restrains yin, softens the liver and relieves pain; licorice is sweet and warm, invigorates the spleen and replenishes qi, relieves spasms and relieves pain. The two medicines work together, sour and sweet to transform yin, harmonize the liver and spleen, and have the effect of softening tendons and relieving pain. [0003] Traditional Chinese medicine fingerprints are one of the main means of quality control of traditional Chinese medicine products, which can more comprehensively reflect t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N30/8686
Inventor 高星张春容曹科李锐赵丽徐志毅王恒
Owner CHENGDU HUASUN GRP INC LTD
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