Method for determining traditional Chinese medicine all-along decoction fingerprint spectra by means of HPLC (high performance liquid chromatography)

A technology of fingerprints and traditional Chinese medicine, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of avoiding singleness and one-sidedness, easy identification, and high technical content

Pending Publication Date: 2019-03-01
合肥创新医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no fast, easy and accurate analytical method that can be used for the identification and content distribution (peak height and peak area) of the number, relative position (retention time) of various chemical components of traditional Chinese medicine Yiguanjian. It characterizes the content of a certain chemical component, and the ratio of the peak height (or peak area) of each peak reflects the relative content of various chemical components) and other related information detection

Method used

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  • Method for determining traditional Chinese medicine all-along decoction fingerprint spectra by means of HPLC (high performance liquid chromatography)
  • Method for determining traditional Chinese medicine all-along decoction fingerprint spectra by means of HPLC (high performance liquid chromatography)
  • Method for determining traditional Chinese medicine all-along decoction fingerprint spectra by means of HPLC (high performance liquid chromatography)

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Experimental program
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Effect test

Embodiment 1

[0037] A kind of fingerprint method that uses HPLC to measure traditional Chinese medicine Yiguanjian that the present invention proposes, comprises the following steps:

[0038] Octadecylsilane bonded silica gel with a particle size of 5 μm is used as a chromatographic column filler, the inner diameter of the chromatographic column is 4.6 mm, and the length of the chromatographic column is 250 mm; acetonitrile is used as mobile phase A, and formic acid aqueous solution with a volume concentration of 0.01% As mobile phase B, 10 μL Yiguanjian sample solution was subjected to gradient elution, wherein the flow rate was 1.0ml / min, the column temperature was 30°C, and then detected by a DAD detector with a detection wavelength of 316nm to obtain figure 1 .

[0039] Among them, the Yiguanjian sample solution is prepared according to the following process: Weigh 2.0g of Yiguanjian dry cream powder and place it in a stoppered conical flask, add 40ml of methanol, ultrasonicate for 15m...

Embodiment 2

[0051] A kind of fingerprint method that uses HPLC to measure traditional Chinese medicine Yiguanjian that the present invention proposes, comprises the following steps:

[0052] Octadecylsilane bonded silica gel with a particle size of 5 μm is used as a chromatographic column filler, the inner diameter of the chromatographic column is 4.6 mm, and the length of the chromatographic column is 250 mm; acetonitrile is used as mobile phase A, and acetic acid aqueous solution with a volume concentration of 0.1% The mobile phase B is used for gradient elution of Yiguanjian sample solution, wherein the flow rate is 1.0ml / min, the column temperature is 30°C, and then detected by a DAD detector with a detection wavelength of 316nm.

[0053] Among them, the Yiguanjian sample solution is prepared according to the following process: Weigh 2.0 g of Yiguanjian dry cream powder, put it in a stoppered conical flask, add 40 ml of methanol, ultrasonicate for 15 minutes, let cool, filter, and put ...

Embodiment 3

[0057] A kind of fingerprint method that uses HPLC to measure traditional Chinese medicine Yiguanjian that the present invention proposes, comprises the following steps:

[0058] Octadecylsilane bonded silica gel with a particle size of 5 μm is used as a chromatographic column filler, the inner diameter of the chromatographic column is 4.6 mm, and the length of the chromatographic column is 250 mm; acetonitrile is used as mobile phase A, and phosphoric acid aqueous solution with a volume concentration of 0.1% The mobile phase B is used for gradient elution of Yiguanjian sample solution, wherein the flow rate is 1.0ml / min, the column temperature is 30°C, and then detected by a DAD detector with a detection wavelength of 316nm.

[0059] Among them, the Yiguanjian sample solution is prepared according to the following process: Weigh 2.0 g of Yiguanjian dry cream powder, put it in a stoppered conical flask, add 40 ml of methanol, ultrasonicate for 15 minutes, let cool, filter, and ...

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Abstract

The invention discloses a method for determining traditional Chinese medicine all-along decoction fingerprint spectra by means of HPLC (high performance liquid chromatography). The method includes steps of carrying out gradient elution on all-along decoction specimen solution by a filler chromatographic column, mobile phase A and mobile phase B; carrying out detection by DAD (diode array detectors). Octadecyl silane chemically bonded silica is used as the filler chromatographic column, a mixture of one or two types of acetonitrile and methyl alcohol is used as the mobile phase A, and one of formic acid aqueous solution, acetic acid aqueous solution and phosphoric acid aqueous solution is used as the mobile phase B. The method for determining the fingerprint spectra by means of HPLC has theadvantages of speediness, simplicity, convenience, comprehensiveness, accuracy and reliability.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for measuring the fingerprint of traditional Chinese medicine Yiguanjian by using HPLC. Background technique [0002] The prescription of "Yiguanjian" comes from "Yifang Jiedu" written by Qian Minji in the Qing Dynasty, "Yiguanjian (Liuzhou) is mainly used for liver and blood decline, epigastric pain, and hypochondriac pain." [0003] The prescription of "Yiguanjian" consists of: North Radix Ginseng, Ophiopogon japonicus, and Angelica are 1 cent and 5 cents each, wolfberry and raw land are 3 cents each, and Toosendan are 2 cents each. [0004] "Yiguanjian" Functions and Indications: It has the effect of nourishing yin and soothing the liver; it is mainly used for liver and kidney yin deficiency, stagnation of liver qi, pain in the chest, abdomen and hypochondriac, acid regurgitation, dry mouth, red tongue with little fluid, weak or weak pul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/54G01N30/74
CPCG01N30/02G01N30/54G01N30/74G01N2030/027
Inventor 曹明成黄顺旺叶强曹阳
Owner 合肥创新医药技术有限公司
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