A method for constructing hplc fingerprint of Xiaoerfeike granule

A technology of fingerprint spectrum and construction method, which is applied in the field of construction of HPLC fingerprint of Pediatric Feike Granules, and achieves the effect of ensuring clinical medication, good absorption value and stable baseline.

Active Publication Date: 2022-01-07
CHANGCHUN RENMIN PHARMA GROUP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the completion of the present invention, there was no report on the method of establishing fingerprints based on the chemical composition contained in Xiaoer Feike Granules

Method used

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  • A method for constructing hplc fingerprint of Xiaoerfeike granule
  • A method for constructing hplc fingerprint of Xiaoerfeike granule
  • A method for constructing hplc fingerprint of Xiaoerfeike granule

Examples

Experimental program
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Effect test

preparation example Construction

[0045] a, preparation of test solution

[0046] Weigh Xiaoerfeike granules, grind them finely, weigh 1-3g, accurately weigh, put in a stoppered Erlenmeyer flask, accurately add 20-50ml of methanol, weigh, ultrasonically treat for 20-40 minutes, let cool, and then Weigh the weight, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the test solution;

[0047] b. Preparation of reference substance solution

[0048] Weigh the reference substances of rutin, liquiritin, hesperidin, quercetin, chrysophanol, and emodin methyl ether respectively, weigh them accurately, use methanol as the solvent, prepare mixed reference substance solutions with different mass concentrations, pass through the microporous filter membrane, take the continued filtrate as the reference substance solution;

[0049] c. Determination

[0050] Precisely draw 5-15 μl each of the reference substance solution and the test solution, inject it into a high-performance liqu...

Embodiment 1

[0056] Embodiment 1: Preparation of Xiaoer Feike Granules

[0057] Pediatric lung cough granule is composed of 20g ginseng, 8g Atractylodes macrocephala, 20g gallinaceous gold, 20g turtle shell, 39g northern sand ginseng, 29g artemisia annua, 8g cassia twig, 8g Danfu tablet, 20g coltsfoot flower, 23g mulberry bark, and 20g astragalus , Poria cocos 20g, tangerine peel 20g, wine rhubarb 12g, Digupi 23g, Zhilicorice 12g, Ophiopogon japonicus 39g, dried ginger 8g, Trichosanthes 29g, aster 20g, Dannanxing 8g, medlar 20g.

[0058] The specific preparation method is as follows: add astragalus, Digupi, Adenophora, Radix Radix Radix, Radix Glycyrrhizae, Artemisia annua, Guizhi, Trichosanthes, Aster, and Morus alba to decoction twice in the above twenty-two flavors. After 2 hours, combine the decoction, filter, and concentrate the filtrate into a clear paste with a relative density of 1.26-1.30 (80°C); the remaining ginseng and other twelve flavors are ground into fine powder, mixed wit...

Embodiment 2

[0059] Example 2: Detection of fingerprints of different batches of children's lung cough particles

[0060] 1. Instruments and reagents

[0061] 1.1 Agilent 1220 high performance liquid chromatograph (Agilent, USA); KQ-100 ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); 124S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0062] 1.2 Purified water (Wahaha), acetonitrile (chromatographically pure), and other reagents are analytically pure. Liquiritin, Quercetin, Emodin, Hesperidin, Chrysophanol, Rutin Reference Substances (Lot No. 111610-201908, 100081-201610, 110758-200610, 110721-201818, 110796-201922, 100080-201811, China Institute for Food and Drug Control). Pediatric Feike Granules (batch numbers: 20180328, 20180707, 20181105, 20190203, 20192406, 20191105, 20190904, 20200302, 20200405, 20201110, Changchun People’s Pharmaceutical Group Co., Ltd.).

[0063] 2. Fingerprint determination

[0064] 2.1 Chromatographic conditions: Octadecylsilane bo...

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Abstract

The invention relates to a method for constructing an HPLC fingerprint of Xiaoerfeike granules, which belongs to the field of medicine. According to the HPLC fingerprint of Xiaoerfeike granules, the invention optimizes the conditions of the mobile phase, detection wavelength, gradient elution procedure and the like, and establishes the detection conditions of the fingerprint. Based on multiple batches of samples, a standard fingerprint of Xiaoerfeike Granules was established, which identified 24 common peaks, and carried out the identification of medicinal materials and components of the related common peaks. The method of the invention can comprehensively control the quality of Xiaoer Feike Granules, so as to better ensure the quality stability, consistency and controllability of Xiaoer Feike Granules, thereby ensuring the safety and effectiveness of Xiaoer Feike Granules . The present invention comprehensively analyzes the status of each component in Xiaoer Feike Granules through systematic identification of components and the attribution of single medicinal materials with common peaks in different batches of samples, which can provide an important reference for the quality control of Xiaoer Feike Granules.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing an HPLC fingerprint of Xiaoer Feike granule. Background technique [0002] Chinese medicine fingerprints use modern analysis techniques to reflect the chemical components in samples by analyzing and comparing the chromatograms of traditional Chinese medicines or proprietary Chinese medicines. It is often used in clinical component analysis and quality evaluation of Chinese medicines. Just like each person's unique fingerprint, each medicinal material also has its own unique medicinal properties and active ingredients. With the help of modern computer and software analysis technology, each medicinal material can be identified like a fingerprint, and the unique active ingredients can be identified, the internal quality of traditional Chinese medicine can be monitored, and the problem of quality standardization can be fundamentally solved. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N30/8686
Inventor 田涵雯黄青陈革
Owner CHANGCHUN RENMIN PHARMA GROUP
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