Construction method of HPLC fingerprint spectrum of granules for treating infantile lung cough

A technology of fingerprint spectrum and construction method, which is applied in the field of construction of HPLC fingerprint of Pediatric Feike Granules, and achieves the effect of ensuring clinical medication, good absorption value and stable baseline.

Active Publication Date: 2021-08-10
CHANGCHUN RENMIN PHARMA GROUP
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the completion of the present invention, there was no report on the method of esta

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of HPLC fingerprint spectrum of granules for treating infantile lung cough
  • Construction method of HPLC fingerprint spectrum of granules for treating infantile lung cough
  • Construction method of HPLC fingerprint spectrum of granules for treating infantile lung cough

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] a, prepared for the test solution

[0046] It is called the psychopatho particles, weighs fine, weighs 1 ~ 3G, precision weighing, putting the plunger bottle, precision adding methanol 20 ~ 50ml, weighing weight, ultrasonic treatment 20 to 40 minutes, cool, then Weigh weight, dissemble weight, shake, filtered, and liquid filtrate, which is allowed to be tested.

[0047] b, prepared for the solution

[0048] It is weighed with rutin, glycidine, orange skin, quercetin, large cranium, large olin methyl ether, precision weighing, with methanol as a solvent, formulated into a mixture of different quality concentrations, over the micropores Filter, polished filtrate as a control solution;

[0049] C, measurement

[0050] Since the test solution is precisely absorbed 5 to 15 μl of the test solution, the high-efficiency liquid chromatography is injected, and the chromatogram within 100 minutes is recorded, and the map is treated with the fingerprint software, that is, the HPLC fing...

Example Embodiment

[0056] Example 1: Preparation of Pediatric Pulph Cough Particles

[0057] Pediatric pneumothar cough particles are ginseng 20g, atractylodes 8g, chicken, 20g, armor 20g, northern sand ginseng 39g, Artemisia, 28g, 4G, Winter flower 20g, mulberry 23g, Astragalus 20g 20g, tangerine peel 20g, wine rhubarin 12g, floor bone skin 23g, licorice 12g, Wheat 39g, dried ginger 8g, melon, purple 20g, gallbladder 8g, medlar 20g preparation.

[0058] The specific preparation method is: the above twenty-two smell of Astragalus, floor bulls, northern sand ginseng, 冬, 甘, artemisia, nationalizes, melon, purple, mulberry plus water, decoction, each 2 hours, combined with decoction, filtrate, the filtrate concentrated into a clear density of 1.26 to 1.30 (80 ° C); the other people's parameters were pulverized into fine powder, and the above cleans, sucrose, mixed, system It is granule, dried, and is 1000 g, which is parental pneumatic cough particles.

Example Embodiment

[0059] Example 2: Detection of fingerprint map of different batch children

[0060] 1, instrument and test

[0061] 1.1Agilent 1220 high performance liquid chromatograph (USA Agilent); KQ-100 ultrasonic cleaning (Kunshan Ultrasonic Instrument Co., Ltd.); 124S Electronic Balance (Beijing Saidolis Timing Co., Ltd.).

[0062] 1.2 Pure Water (Wahaha), acetonitrile (chromatographic pure), other reagents are analyzed. Glycerin, quercetin, large olin methyl ether, orange peletin, large yellowol, rutin gas (batch number 111610-201908, 11081-201610, 110758-200610, 110721-201818, 110796-201922 ,100080-201811, China Food and Drug Test Research Institute). Pediatric pneum cough particles (batch number: 20180328, 20180707, 20181105, 20180, 2010, 20192406, 20191105, 20190904, 20200302, 20200405, 20201110, Changchun People Pharmaceutical Group Co., Ltd.).

[0063] 2, fingerprint map measurement

[0064] 2.1 Chromatographic conditions: The chromatography column is a filler with an octadylsilane, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a construction method of an HPLC fingerprint spectrum of granules for treating infantile lung cough, and belongs to the medicine field. Aiming at the HPLC fingerprint spectrum of the children lung cough granules, conditions such as a mobile phase, a detection wavelength and a gradient elution procedure are optimized, and fingerprint spectrum detection conditions are established. A standard fingerprint spectrum of the children lung cough granules is established according to multiple batches of samples, 24 common peaks are confirmed in the spectrum, and medicinal material affiliation and component identification are carried out on the related common peaks. According to the method disclosed by the invention, the quality of the children lung cough granules can be relatively comprehensively controlled, so that the quality stability, consistency and controllability of the children lung cough granules are better ensured, and the safety and effectiveness of the children lung cough granules are ensured. According to the method, through systematic component identification and the attribution of single medicinal materials with common peaks in different batches of samples, the current situation of each component in the children lung cough granules is analyzed more comprehensively, and an important reference basis can be provided for quality control of the children lung cough granules.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing an HPLC fingerprint of Xiaoer Feike granule. Background technique [0002] Chinese medicine fingerprints use modern analysis techniques to reflect the chemical components in samples by analyzing and comparing the chromatograms of traditional Chinese medicines or proprietary Chinese medicines. It is often used in clinical component analysis and quality evaluation of Chinese medicines. Just like each person's unique fingerprint, each medicinal material also has its own unique medicinal properties and active ingredients. With the help of modern computer and software analysis technology, each medicinal material can be identified like a fingerprint, and the unique active ingredients can be identified, the internal quality of traditional Chinese medicine can be monitored, and the problem of quality standardization can be fundamentally solved. [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N30/8686
Inventor 田涵雯黄青陈革
Owner CHANGCHUN RENMIN PHARMA GROUP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap