Fingerprint construction method and detection method of angelica sinensis six-yellow decoction composition

A technology of Danggui Liuhuang Decoction and fingerprints, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that there is no report on the quality control of component analysis, and there are not many Danggui Liuhuang Decoctions.

Inactive Publication Date: 2021-03-26
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many studies on the fingerprints of Danggui Liuhuang Decoction, and there are no reports on the composition analysis and quality control of this prescription.

Method used

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  • Fingerprint construction method and detection method of angelica sinensis six-yellow decoction composition
  • Fingerprint construction method and detection method of angelica sinensis six-yellow decoction composition
  • Fingerprint construction method and detection method of angelica sinensis six-yellow decoction composition

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Experimental program
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preparation example Construction

[0092] The preparation methods of the above two Danggui Liuhuang Decoction compositions correspond to the production processes of the two Danggui Liuhuang Decoction composition preparations. The Danggui Liuhuang Decoction compositions obtained by the two preparation methods can be used as test samples. It can be understood that the Danggui Liuhuang Decoction composition obtained by other production processes can be used as the tested product.

[0093] Correspondingly, the present invention provides a fingerprint detection method of the composition of Angelica Liuhuang Decoction, comprising the following steps:

[0094] The Danggui Liuhuang Decoction composition to be tested is made into a sample solution to be tested;

[0095] Inject the sample solution to be tested into a liquid chromatograph for chromatographic analysis to generate a spectrum of the sample to be tested;

[0096] The standard fingerprint in the fingerprint construction method of the above-mentioned Danggui ...

Embodiment 1

[0130] The preparation of embodiment 1 reference substance solution

[0131] Ferulic acid reference substance solution: Take an appropriate amount of ferulic acid reference substance, weigh it accurately, add 70% methanol to make a ferulic acid solution containing 50 μg per 1 ml.

[0132] Baicalin reference substance solution: Take an appropriate amount of baicalin reference substance, weigh it accurately, add 70% methanol to make a baicalin solution containing 50 μg per 1 ml.

[0133] Berberine hydrochloride reference substance solution: take an appropriate amount of berberine hydrochloride reference substance, weigh it accurately, add 70% methanol to make a berberine hydrochloride solution containing 50 μg per 1 ml.

[0134] Phellodendrine hydrochloride reference substance solution: get an appropriate amount of Phellodendron hydrochloride reference substance, accurately weighed, and add 70% methanol to make a phellodine hydrochloride solution containing 50 μg per 1 ml.

[0...

Embodiment 2

[0137] The preparation of embodiment 2 need testing solution

[0138] There are two preparation methods of the Danggui Liuhuang Decoction composition, and the first Danggui Liuhuang Decoction composition and the second Danggui Liuhuang Decoction composition are prepared by using the two preparation methods.

[0139] The preparation method of the first Angelica Liuhuang Decoction composition is as follows:

[0140] a. Weigh the following decoction pieces in parts by weight: Angelica 4g, Rehmannia glutinosa 4g, Rehmannia glutinosa 4g, Scutellaria baicalensis 4g, Coptis chinensis 4g, Cortex Phellodendron 4g, Radix Astragali 8g;

[0141] b. Put the weighed decoction pieces together in a 2L casserole, add 600ml of water, soak for 30 minutes, cover and boil, keep slightly boiling until the decoction is 300-400ml, filter it with a 200-mesh filter cloth while it is hot, and obtain the filtrate;

[0142] c. After the filtrate is concentrated under reduced pressure, the steps of freeze...

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Abstract

The invention discloses a fingerprint construction method and a detection method of an angelica sinensis six-yellow decoction composition, and the construction method comprises the following steps: (1) taking 5-hydroxymethylfurfural, chlorogenic acid, phellodendrine hydrochloride, ferulic acid, baicalin and berberine hydrochloride as reference substances, and respectively preparing reference substance solutions; (2) taking angelica sinensis, radix rehmanniae recen, prepared rehmannia root, scutellaria baicalensis, coptis chinensis, golden cypress and astragalus membranaceus to prepare an angelica sinensis six-yellow decoction composition, and preparing the angelica sinensis six-yellow decoction composition into a test solution; and (3) precisely absorbing the test solution and the reference solutions, injecting the test solution and the reference solutions into a liquid chromatograph for chromatographic analysis with a detection wavelength of 280-320 nm to obtain a test fingerprint anda reference chromatogram, and formulating a standard fingerprint of the angelica sinensis six-yellow decoction composition. The fingerprint construction method can accurately detect the standard fingerprint spectrum of the quality of the angelica sinensis six-yellow decoction composition, and can effectively control the product quality of the angelica sinensis six-yellow decoction preparation.

Description

technical field [0001] The invention relates to the technical field of quality detection of Chinese patent medicines, in particular to a fingerprint construction method and a detection method of a composition of Danggui Liuhuang Decoction. Background technique [0002] Danggui Liuhuang Decoction comes from the "Orchid House Secret Collection" compiled by Li Dongyuan, the four masters of the Jin and Yuan Dynasties. It is known as "the holy prescription for curing night sweats" by later generations. Astragalus has a total of seven herbs, among which angelica, rehmannia and rehmannia are the monarch drugs. Angelica is sweet and pungent and warm, and has the effect of nourishing blood. Coptis chinensis tastes bitter and cold to clear the scorched fire in the middle, Cortex Phellodendron tastes bitter and cold and relieves the scorched fire, and it is a ministerial drug; Astragalus is an adjuvant drug, which can benefit qi and strengthen the surface. Indications for night sweat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8631G01N30/8686
Inventor 曾杉林碧珊梁浩明陈锦霞徐晖史紫娟陈伟钢陈科成高永坚汤春花
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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