47 results about "Phellodendrine" patented technology

A composite vegetative estrin medicine in the form of suppository, gel or film for treating vagititis and improving the toughness of vagina and its resistance to bacteria is proportionally prepared from vegetative estrin, cnidiadin, borneol, and at least one of matrine, phellodendrine, berberine, etc and their physiocologically acceptable salts.

The invention discloses a method for separating and purifying phellodendrine monomer, which comprises the following steps of: extracting total alkaloid; removing berberine by means of acid deposition; filtering and further removing; separating phellodendrine monomer through a preparative high-efficiency liquid chromatogram; extracting dichloromethane; and drying to prepare the phellodendrine monomer. The method has simple operation, short preparation period, high separation efficiency, stable technology and low cost, and can separate the large number of phellodendrine monomer with high purity to obtain the phellodendrine monomer, the purity of which is more than 98%.

The invention discloses a pharmaceutical composition for preventing and treating rheumatoid arthritis. The traditional Chinese medicine raw materials for constituting active ingredients of the pharmaceutical composition by weight are as follows: 5.5-6.0mg of berberine, 0.3-0.4mg of palmatine, 1.5-2.0mg of jatrorrhizine, 1.5-1.8mg of magnoflorine, 0.1-0.2mg of phellodendrine, 0.2-0.3mg of coptisine, 55-60mg of baicalin, 1.5-2.0mg of baicalein, 5.0-7.0mg of wogonoside, 0.03-0.05mg of wogonin, 20-25mg of geniposide, 0.1-0.2mg of crocin and 0.25-0.3mg of chlorogenic acid. The results of pharmacological tests show that the pharmaceutical composition can significantly reduce the disease severity of CIA mice with the rheumatoid arthritis, significantly improve the inflammation and the immune injury situation at lesion sites in comparison with a control group and be used for preparing drugs for preventing and treating the rheumatoid arthritis.

The invention belongs to the technical field of natural medicine extraction, and relates to a phellodendrine monomer and a preparation method of salt thereof. The method comprises the steps of: preparing the phellodendrine monomer through acid chloride solution extraction, macroporous adsorption resin enrichment, alumina column chromatographic purification and the like; and recrystallizing the phellodendrine monomer so as to obtain a phellodendrine salt monomer. The method has the advantages of low preparation cost, short period and high prepared product purity, and is convenient to operate.

The invention relates to the field of Chinese medicines and discloses Chinese medicinal capsules for treating prostatitis and prostatic hyperplasia and a preparation method for the Chinese medicinal capsules. By the preparation method, the transfer rates of eight medicinal active ingredients, namely phellodendrine, tanshinone IIA, salvianolic acid B, paeoniflorin, amarogentin, linderane, vaccarin and imperatorin in phellodendron, red paeony root, root of red-rooted salvia, peach kernel, root of combined spicebush, cowherb seed and angelica dahurica are all improved to over 95 percent, and are remarkably higher than the transfer rate obtained by the conventional method in China, so that the medicinal effect is improved and the cost is reduced.

The invention discloses a preservative for loquat fruits, and belongs to the field of preserving of fruits. The preservative is prepared from the following components in parts by weight: 10 to 30 parts of phytic acid, 12 to 25 parts of cinnamon, 3 to 12 parts of betel nut, 4 to 15 parts of clove, 2 to 10 parts of tea polyphenol, 4 to 12 parts of phellodendrine, and 2 to 11 parts of a gastrodia elata extract. The preservative is long in preserving cycle for loquat fruits, does not change the original taste of loquat fruits, and is safe to use; the adopted raw materials are harmless to a human body; the preparation is simple, and the cost is low.

The invention provides a method for analyzing all components of an angelica sinensis six-yellow decoction, and belongs to the technical field of pharmaceutical analysis. An ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology is adopted to detect an angelica sinensis six-yellow decoction sample, and 15 chemical reference substances are adopted at the same time: baicalin, wogonoside, berberine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, phellodendrine hydrochloride, astragaloside, calycosin-7-glucoside, ferulic acid, chlorogenic acid, ligustilide, verbascoside, baicalein and wogonin are adopted to confirm a detection result of the angelica sinensis six-yellow decoction andclearly identify 68 components; comprehensive analysis of the components of the angelica sinensis six-yellow decoction is realized, and the reliability of quality control is improved.

The invention relates to a construction method and a detection method of a HPLC feature map of a golden cypress medicinal material. The construction method comprises the following steps: reference solution preparation: preparing a golden cypress control medicinal material reference solution and a berberine hydrochloride and phellodendrine hydrochloride reference substance solution; sample solutionpreparation: preparing a golden cypress medicinal material sample solution and a decoction sample solution; high-performance liquid chromatography test: absorbing the control medicinal material reference solution, the reference substance solution, the medicinal material sample solution and the decoction sample solution; injecting into a liquid chromatograph, comparing a medicinal material samplemap with a decoction sample map, determining water soluble common peaks and obtaining the UPLC feature map of the golden cypress medicinal material. The HPLC feature map obtained by the construction method not only can qualitatively and quantitatively analyze the quality of the golden cypress medicinal material, but also can reflect the quality of golden cypress standard decoction as a reference.

The invention relates to a detection method of an danggui liuhuang decoction, and the detection method comprises a fingerprint spectrum and a content determination method. The fingerprint spectrum detection method comprises the following steps: a, constructing a control fingerprint spectrum of the danggui liuhuang decoction; b, employing the control fingerprint spectrum of the danggui liuhuang decoction for similarity evaluation of the danggui liuhuang decoction fingerprint spectrum detection. The content determination method comprises the step of determining the content of baicalin, coptisine, berberine, phellodendrine and astragaloside in the angelica sinensis six-yellow soup. According to the method, the fingerprint spectrum and multi-component content determination are established at the same time, the overall quality control of the angelica sinensis six-yellow soup can be realized, and the repeatability, stability, durability and the like of the method are relatively good.

The invention relates to a SPE-HPLC method for determining a content of phellodendrine in Leshu washing lotion. The method comprises the following steps of: A, preparing aliquid of a reference substance; B, preparing aliquid of a test sample: preparing the liquidof the test sample by adopting a solid phase extraction method; C, performing the liquid chromatographic detection; D, establishing a standard curve; and E, determining a sample. The testedliquid is prepared by selecting the solid phase extraction method, the extraction recovery rate about 100 percent can be obtained, inorganic salts and organic impurities in a test sample can be effectively removed, and the service life of a chromatographic column can be prolonged. The solid phase extraction method can process the sample in batches and is easy in automation, so that the working efficiency can be improved. A majority of methods reported by the existing literatures adopt a C18 chromatographic column, and the present method selects an amino column as an analysis column, so thatcompared with the C18 chromatographic column, the amino column has the advantages of high column efficiency, sharp peak pattern and little interference. The method is simple in operation, accurate in result, good in repeatability and less in impurity interference in the analysis process.

The invention relates to a pharmaceutical composition. The pharmaceutical composition is prepared from phellodendrine, paeoniflorin and optional EGCG (epigallocatechin gallate). The pharmaceutical composition can prevent or treat brain tissue damage caused by local and whole cerebral ischemia, can specifically prevent or treat cerebral hemorrhage, cerebral infarction, cerebral thrombosis and otherdiseases, especially has a very good treatment effect on cerebral infarction, and is high in safety and free from side effect.

The invention provides a method for establishing a fingerprint spectrum of a bactericidal itching-relieving lotion. The method comprises the following steps: S1, preparing a mixed reference substancesolution, namely precisely weighing matrine, astilbin, phellodendrine, berberine hydrochloride, cnidium lactone and atractylodes lancea reference substances respectively, and adding methanol to prepare the mixed reference substance solution; S2, preparing a bactericidal itching-relieving lotion sample solution; S3, preparing a single reference medicinal material solution; and S4, determining. Thefingerprint spectrum calibrates 16 common peaks and determines the five medicinal materials belonging to a prescription, the established fingerprint spectrum has high technical content, and the singleness and one-sidedness of the existing quality control method are avoided. An effective quality control basis is provided for the production of the bactericidal itching-relieving lotion, and a foundation is laid for the research on the correlation between the pharmacodynamic material basis and the pharmacological action of the bactericidal itching-relieving lotion.

The invention discloses a method for separating phellodendrine glucuronic acid conjugate from urine. The method comprises the steps that a urine sample is subjected to AB-8 macroreticular resin separation twice, and an ODS open column is utilized for enriching metabolites; a Sephadex LH20 gel column is utilized for purification; obtained flow components are prepared into a target product by utilizing an Agilent 1200 type preparation solution, and LC-MSn analysis verification is utilized for identifying the structure of the product. According to the method, through separation and extraction, the in-vivo phellodendrine metabolite is obtained, the chemical structure of the in-vivo phellodendrine metabolite is determined, quantitative analysis is conducted, and feasibility conditions and experiment bases are provided for analysis of the fragmentation pattern and conversion relationship of the in-vivo phellodendrine metabolite and the in-vivo pharmacokinetic study and pharmacologic action mechanism study of the in-vivo phellodendrine metabolite. The method has the advantages of simple operation, low cost, high efficiency and the like.

The invention provides a quality control method of a traumatology department yellow-water preparation. The method comprises the following steps of (1) weighing phellodendrine chloride, jatrorrhizine hydrochloride, epiberberine chloride, coptisine chloride, palmatine chloride, berberine hydrochloride, geniposide and chlorogenic acid; adding absolute methanol or methanol water solution for preparinga reference product solution; (2) taking contents of the traumatology department yellow-water preparation to be tested; adding absolute methanol or methanol water solution for dissolution; performingfiltering; taking subsequent filtering liquid to be as a solution to be tested; (3) respectively taking the reference product solution and 1 to 5 muL of the solution to be tested; injecting the materials into a liquid chromatography-mass spectrometry instrument for detection; obtaining the contents of phellodendrine, jateorhizine, epiberberine, coptisine, palmatine, berberine, geniposide and chlorogenic acid through calculating a quantitative chromatogram map. The method has the advantages that the original deficient quantitative evaluation index can be completed by once quantitative determination. The quantitative chromatogram map of the method can show good wave peak separation; the identification by the method is fast and accurate.

The invention discloses a cortex phellodendron chinese identification method. According to the identification method, cortex phellodendron chinese is used for preparing a solution of a test product, solutions in which a berberine hydrochloride reference substance, a phellodendrine chloride reference substance and a magnoflorine reference substance are dissolved are used as reference substance solutions, and through high performance liquid chromatograph measurement, liquid chromatograms of the solution of the test product and the reference substance solutions are obtained respectively; the obtained liquid chromatograms are guided into a traditional Chinese medicine chromatograph fingerprint similarity evaluation system for analysis, through analysis, a cortex phellodendron chinese fingerprint is obtained, and hierarchical cluster analysis or principal component analysis or partial least square analysis is adopted for processing data of the cortex phellodendron chinese fingerprint, so that classification identification is conducted on the cortex phellodendron chinese or processed products of the cortex phellodendron chinese. According to the cortex phellodendron chinese identification method, on the basis of building the cortex phellodendron chinese fingerprint, the cortex phellodendron chinese index component content is measured, and by combining different chemical mode identification methods, the cortex phellodendron chinese, the processed products of the cortex phellodendron chinese and fake cortex phellodendri amurensis can be effectively identified and distinguished.

The invention discloses application of phellodendrine or pharmaceutical salt of the phellodendrine in the tumor treatment aspect. The application finds that the phellodendrine or the pharmaceutical salt of the phellodendrine has the inhibiting effect on breast cancer, colon cancer, liver cancer, gastric cancer, KRAS mutant pancreatic cancer and leukemia, has no obvious inhibition on human normal cells and is hopeful to be developed into low-toxicity anti-tumor medicine, wherein by inhibiting the macropinocytosis effect of KRAS mutant pancreatic cancer cells and reducing the Bcl-2/Bax expression ratio in the KRAS mutant pancreatic cancer, the phellodendrine or the pharmaceutical salt of the phellodendrine inhibits the multiplication of the KRAS mutant pancreatic cancer and induces the KRASmutant pancreatic cancer to apoptosis. Therefore the phellodendrine or the pharmaceutical salt of the phellodendrine can be developed into a medicine preparation for resisting breast cancer, colon cancer, liver cancer, gastric cancer, KRAS mutant pancreatic cancer or leukemia; the preparation contains the phellodendrine, the pharmaceutical salt of the phellodendrine or prodrug of the phellodendrine, also contains a pharmaceutically acceptable carrier or excipient and is prepared into a pharmaceutically acceptable dosage form.

The invention relates to an improved Longqing capsule quality detection method. The detection method comprises the following steps: carrying out thin-layer identification inspection on coptis chinensis, cortex phellodendri, patrinia herb and rhizoma alismatis medicinal materials in a capsule, and determining the contents of phellodendrine hydrochloride, 23-acetyl alisol B and 23-acetyl alisol C in the capsule. Through methodological investigation, the detection method has the effects of being high in specificity, good in durability and good in repeatability, the quality condition of the Longqing capsules can be effectively detected, and the product quality is further improved.

The invention provides a thin-layer chromatography identification method of a Shenan capsule, which comprises the following steps: carrying out thin-layer chromatography identification on the Shenan capsule, and judging whether one or more of chalepin acetate, ursolic acid, atractylodis ketone, liquiritin and phellodendrine hydrochloride are contained or not. Compared with the existing Shenan capsule medicine standard, the qualitative identification indexes of five representative components are increased, the identification method is more comprehensive in contrast, high in specificity, intuitive and clear in judgment and objective and reliable in conclusion, and a technical method is provided for perfecting and improving the quality standard of the national medicine Shenan capsule.

The invention relates to a medicine composition for treating hemorrhoids, medicinal preparations and application. The medicine composition and the medicinal preparations contain individual ingredients, such as honeysuckle stem, lightyellow sophora root, cortex phellodendri and Chinese gall. The medicine composition is prepared from 0.40 to 0.66 part by weight of loganin, 0.47 to 0.66 part by weight of matrine, 1.15 to 1.48 parts by weight of berberine, 0.12 to 0.16 part by weight of phellodendrine, 53.46 to 65.34 parts by weight of gallic acid, 1.86 to 2.66 parts by weight of osthole and 4.79to 5.85 parts by weight of total digua fig stem and leaf flavone. All the individual Chinese herbal medicine ingredients are chosen, excipients are added, and the medicinal preparations are prepared according to conventional preparation processes. The medicine composition and the medicinal preparations prepared therefrom disclosed by the invention have the effects of removing toxin and eliminatingdampness and stopping bleeding and astringing sores, and can achieve a good therapeutic effect when applied to internal hemorrhoids, external hemorrhoids and mixed hemorrhoids caused by the syndromeof accumulated dampness-heat and manifested uncomfortable symptoms such as bleeding, swelling, pain and itching, moreover, toxicity is low, and therefore the medicine composition and the medicinal preparations are ideal medicines.

The invention relates to a preparation method of an Angelica sinensis-Liuhuang preparation. The method can improve the content of phellodendrine in the Angelica sinensis-Liuhuang preparation. According to the method, angelica sinensis, rehmannia, prepared rehmannia root, scutellaria baicalensis and astragalus membranaceus are separately treated with coptis chinensis and golden cypress, a membrane filtration technology is introduced to purify an extracting solution, so that volatile components in the angelica sinensis, the rehmannia, the prepared rehmannia root, the scutellaria baicalensis and the astragalus membranaceus are retained, the medicine effect is improved, volatile components in the angelica sinensis, the rehmannia, the prepared rehmannia root, the scutellaria baicalensis and the astragalus membranaceus are concentrated under the room temperature condition through a spiral-wound membrane ultrafiltration technology, and the volatile components and activity thereof are reserved to the maximum extent, the content of the phellodendrine in a Chinese angelica six-yellow preparation prepared by the process is remarkably improved compared with that of the Chinese angelica six-yellow preparation prepared by a traditional process, and HPLC content determination shows that the content of the phellodendrine in the Chinese angelica six-yellow preparation prepared by the process is not less than 0.3 mg/g, the content of berberine is not less than 4 mg/g, the total content of baicalin and wogonoside is not less than 15 mg/g, and the content of total volatile oil is not less than 0.1% (ml/g).