Raw fleece-flower root HPLC fingerprint detection method
A technology for producing Polygonum multiflorum and detection method, which can be applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in comprehensively evaluating the real quality of traditional Chinese medicine and easy mutual influence, and achieve good separation, high precision, and many peaks. Effect
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Embodiment 1
[0053] The screening of embodiment 1 experimental conditions
[0054] 1. Screening of mobile phase conditions
[0055] Under the same sample injection conditions of Radix Polygoni Multiflori, 0.1% formic acid water and acetonitrile were used as pump A and pump B of the eluting mobile phase, and the gradient elution conditions were carried out with different volume ratios (v / v) of A and B in different time periods Screening, gradient elution procedures are shown in Table 2, and the corresponding fingerprints of Radix Polygoni Multiflori are obtained in Table 2. figure 1 (A-F), by figure 1 It can be seen that figure 1 A basically failed to separate; figure 1 B, C, D part of the time point map can not be separated; figure 1 In part of the period of time in E, the baseline drifts seriously, figure 1 In F, each component is well separated, and the baseline is basically stable, so the gradient elution condition is set as the fingerprint analysis condition of Radix Polygon...
Embodiment 2
[0066] Embodiment 2 The method of the present invention and its methodology verification
[0067] 1 Preparation of sample solution
[0068] 25 batches of raw Shouwu commercially available were beaten into coarse powder for later use. Accurately weigh 0.25 g of each coarse powder, put it in a stoppered Erlenmeyer flask, add 20 times the amount of 50% ethanol aqueous solution, soak for 30 minutes, ultrasonically extract at 40°C for 60 minutes, and dilute to 5 mL. Filter through a 0.45 μm microporous membrane, and take the subsequent filtrate for injection.
[0069] 2.2 Preparation of reference solution
[0070] Accurately weigh the reference substances gallic acid and proanthocyanidin B 1 , catechin, gallic acid ester, aloe-emodin glycoside, polydatin, stilbene glycoside, rhein, resveratrol, emodin glycoside, emodin methyl ether glycoside, aloe-emodin, rhein, chrysophanol , emodin, and emodin methyl ether reference substances in a 10mL volumetric flask, set the volume to the...
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