Method for constructing HPLC (high performance liquid chromatography) fingerprint spectrum of medicinal leaves and twigs of rhododendron mariae
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A technology of Rhododendron violaceum and fingerprint spectrum, which is applied in the field of medicine and achieves the effects of easy identification, high technical content and high similarity
Active Publication Date: 2019-09-24
CHANGCHUN UNIV OF CHINESE MEDICINE
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Before the present invention, there is no report on the method of establishing fingerprints with the contained chemical composition of Rhododendron azalea as an index
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Embodiment 1
[0028] Example 1: Detecting the fingerprints of different batches of Rhododendron azalea
[0031] 1.2 Purified water (Wahaha), acetonitrile (chromatographically pure), and other reagents are analytically pure. Rutin, quercetin, hyperoside, quercetin, kaempferol, zidonolic acid reference substance (batch number 100080-200306, 111538-200302, 111521-201205, 100081-200907, 110861-200304, 110709- 200304, China National Institutes for Food and Drug Control). Rhododendron violet (lot numbers: 181001, 181002, 181003, 181004, 181005, 181101, 181102, 181103, 181104, 181105).
[0032] 2 Determination of fingerprint
[0033] 2.1 Chromatographic conditions: Octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, gradient elution, flow rate 1...
Embodiment 2
[0044] Example 2: Determination and data analysis of fingerprints of 13 batches of Rhododendron violet
[0045] Get 13 batches of Rhododendron viola, detect according to the conditions of Example 1, and obtain the HPLC spectra of 13 batches of samples. Such as image 3 .
[0046] Through the comparison of 13 batches of PHLC spectra, the similarity evaluation was carried out to determine its characteristic common peaks: import the chromatograms of 10 batches of Rhododendron violet into the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" 2004A Edition (National Pharmacopoeia Commission), and use the common peaks mode, set the time window width to 0.1min, and generated standard fingerprints by the average method, and determined 16 common peaks. The similarities of the 13 batches of Rhododendron viola were all greater than 0.9, as shown in Table 2. The relative retention times of the 16 common peaks were basically the same, but there were large diff...
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Abstract
The invention provides a method for constructing the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of rhododendron mariae. The method comprises the following steps of: (1) test solution predation: the leaves and twigs of the rhododendron mariae are crushed and weighed, an obtained substance is arranged in an extractor, petroleum ether is added to the extractor, heat reflux extraction is carried out, and the petroleum ether is discarded, and methanol is added, heating reflux is carried out, methanol is supplemented, and filtering is carried out; (2) reference substance solution preparation: rutin, quercitrin, hyperoside, quercetin, kaempferol, an oleanolic acid reference substance are selected, and a mixed reference substance solution is prepared with methanol adopted as a solvent; and (3) and measurement: the reference substance solution and the test solution are injected into a liquid chromatograph separately, and chromatograms are recorded in 105 minutes, and the chromatograms are processed with fingerprint spectrum software, so that the fingerprint spectrum of the leaves and twigs of the rhododendron mariae can be obtained. With the method for constructing the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of the rhododendron mariae of the invention adopted, the common model of the HPLC (high performance liquid chromatography) fingerprint spectrum of the medicinal leaves and twigs of the rhododendron mariae is established, and 16 common peaks are calibrated, so that the quality of the medicinal leaves and twigs of the rhododendron mariae can be effectively characterized; the singleness and one-sidedness of an original quality control method are avoided. The method has a relatively high application value.
Description
technical field [0001] The invention belongs to the field of medicine, and in particular relates to the establishment of a high-performance liquid phase fingerprint of Rhododendron argentina medicinal material by using high-performance liquid chromatography to control the quality of Rhododendron argentina medicinal material. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the spectrum of common peaks that can mark the group characteristics of various components in the traditional Chinese medicine and its preparations, which are obtained after proper processing of Chinese medicinal materials and detected by certain analytical methods and instruments. Through the formulation and comparison of the relative retention time and content or ratio of the overall characteristic peaks of the fingerprint, the quality of Chinese medicinal materials and Chinese medicinal products can be effectively controlled to ensure the relative stability of product q...
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