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Establishment method of lindley eupatorium herb formula granule fingerprint as well as standard fingerprint and application thereof

A standard fingerprint and formula granule technology, which is applied in the field of drug detection, can solve the problems of high concentration of the test product and the inability to fully reflect the quality of the product, and achieve the effect of optimized separation conditions, good feasibility, and stable baseline

Active Publication Date: 2022-03-22
SHINEWAY PHARMA GRP LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method also has the above-mentioned problems that it cannot fully reflect the quality of the product, the concentration of the test product is relatively large, and the content of other extracts exceeds the detection range.

Method used

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  • Establishment method of lindley eupatorium herb formula granule fingerprint as well as standard fingerprint and application thereof
  • Establishment method of lindley eupatorium herb formula granule fingerprint as well as standard fingerprint and application thereof
  • Establishment method of lindley eupatorium herb formula granule fingerprint as well as standard fingerprint and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 A kind of method for constructing the fingerprint of Yema chasing formula granules

[0067] In this example, Yemazhui formula granules are used to construct the fingerprint of Yemazhui formula granules. The specific preparation method of Yemazhui formula granules is as follows:

[0068] Take Ye Ma Zhui decoction pieces, add water to decoct, filter, concentrate the filtrate into a clear paste, add appropriate amount of auxiliary materials, dry (or dry, crush), then add appropriate amount of auxiliary materials, mix well, and granulate to obtain.

[0069] This embodiment is a method for constructing the fingerprint of the wild horse formula particles, including the following specific steps:

[0070] 1) Preparation of test solution, reference medicinal solution and reference solution

[0071] Need testing solution: get Ye Ma Zhui formula granules and grind finely (generally can pass through No. 6 sieve after grinding), accurately weigh 0.5g therefrom, place i...

Embodiment 2～3

[0091] Embodiment 2-3 The construction method of the fingerprint of the wild horse formula granule

[0092] Embodiments 2 to 3 are respectively a method for constructing the fingerprint of the wild horse formula particles. Their steps are basically the same as in Example 1, and the only difference is that the process parameters are different. See Table 1 for details:

[0093] List of each technological parameter in the embodiment 2~3 of table 1

[0094]

[0095] The content of the other parts of Embodiments 2-3 is the same as that of Embodiment 1.

Embodiment 4

[0096] The standard fingerprint spectrum of embodiment 4 wild horse pursuit formula granule

[0097] Using the method for constructing the fingerprints of Yema Zhui formula granules in Example 1, measure the fingerprints of 6 batches of Yema Zhui formula granules, see Figure 4 , the retention time of each peak in the fingerprint chromatogram of each batch of obtained wild horse pursuit formula granules is shown in Table 2;

[0098] Each peak retention time in the fingerprint chromatogram of table 2 each batch of Yema Zhui formula granules

[0099]

[0100]

[0101] Using the National Pharmacopoeia Committee "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 Edition)" to synthesize the fingerprints of the above-mentioned 6 batches of Yema Zhui formula granules, and generate a standard fingerprint of Ye Ma Zhui formula granules composed of 10 characteristic peaks, see Figure 5 . Wherein, with No. 1 peak hyperin as reference peak, calculate...

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Abstract

The invention provides a construction method of a lindley eupatorium herb formula granule fingerprint spectrum, a standard fingerprint spectrum of the lindley eupatorium herb formula granule and application of the standard fingerprint spectrum, and belongs to the technical field of medicine detection.The construction method comprises the steps that a test solution, a reference medicinal material solution and a reference substance solution are prepared, acetonitrile (A) and a 0.1-0.3 wt% phosphoric acid aqueous solution (B) are jointly used as mobile phases, and the standard fingerprint spectrum of the lindley eupatorium herb formula granule is obtained. Performing high performance liquid chromatography detection to obtain a fingerprint spectrum of the lindley eupatorium herb formula granules; the standard fingerprint spectrum of the lindley eupatorium herb formula granules is obtained by using the construction method, and the standard fingerprint spectrum is used for quality evaluation or control in the whole process of research / development / production / clinical application of the lindley eupatorium herb formula granules. According to the method for constructing the fingerprint spectrum of the lindley eupatorium herb formula granules, main medicinal material components in the lindley eupatorium herb formula granules can be detected only through high performance liquid chromatography, and the quality condition of the lindley eupatorium herb formula granules can be comprehensively reflected.

Description

technical field [0001] The invention relates to a drug detection technology, in particular to a method for constructing a fingerprint of a wild horse formula granule, a standard fingerprint and its application. Background technique [0002] The characteristic map is to establish a chromatogram or spectral map for analyzing a certain type or several types of components in the measured substance. At present, the characteristic map has become an internationally recognized effective component for controlling the quality of traditional Chinese medicine or natural medicine, and it is a hot issue in the field of traditional Chinese medicine. In recent years, people have gradually realized that the quality control method formed only for a certain chemical component, It can no longer properly reflect the intrinsic quality of the product. As a multi-indicator quality control mode, the characteristic map of traditional Chinese medicine can better control the intrinsic quality of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/88
CPCG01N30/02G01N30/06G01N30/74G01N30/88G01N2030/8809
Inventor 李雪利高杰曾永祯孙福仁高晗廖明丽李倩董博宇李军山王亚涛张睿智牛金伟张岩岩路晓
Owner SHINEWAY PHARMA GRP LTD
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