Method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotata

A technology for iridoid glycosides and phenylethanol glycosides, which is applied in the field of simultaneous detection of iridoid glycosides, flavonoids, caffeoyl components, and phenylethanol glycosides in the unique flavor, to achieve accurate quality control, effective detection, and good separation Effect

Inactive Publication Date: 2014-12-24
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the simultaneous determination of more than 3 components in Duyiwei

Method used

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  • Method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotata
  • Method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotata
  • Method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotata

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Effect test

Embodiment 1

[0022] Embodiment 1 Detection method of the present invention

[0023] (1) Take the single medicinal material to be tested, extract it with 60% v / v methanol, and prepare the test solution;

[0024] (2) Select shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester, chlorogenic acid, forsythiaside B, ergoside, rutin and luteolin as reference substances, and prepare reference substance solutions;

[0025] (3) Inject the reference substance and the test solution into the high-performance liquid chromatograph respectively, and detect the above-mentioned 7 components in Duyiwei according to the external standard method, and the chromatographic conditions are as follows:

[0026] Chromatographic column: Octadecyl bonded silica gel as filler

[0027] Detection wavelength: the detection wavelength of shanzhiside methyl ester and 8-O-acetyl shanzhiside methyl ester is 238nm; the detection wavelength of chlorogenic acid, forsythiaside B and ergoside is 330nm; the detection wavele...

Embodiment 2

[0030] Embodiment 2 Methodological investigation

[0031] 1 material

[0032] Utimate 3000 high performance liquid chromatography (including quaternary gradient pump, online degasser, autosampler, column thermostat, VWD3400 UV detector, Chromeleon chromatography workstation, Diana, Germany), Sartorius BS224S electronic analytical balance (Germany Doris), MILLIQ ultrapure water machine (Millipore, USA)

[0033] Shanzhiside methyl ester, chlorogenic acid, 8-O-acetylshanzhiside methyl ester, forsythiaside B, rutin, ergosteroside, and luteolin reference substances were all purchased from Chengdu Master Biotechnology Co., Ltd. (for content determination, purity ≥ 98%). Acetonitrile (chromatographically pure, Fisher), water was ultrapure water, and other reagents were analytically pure.

[0034] Duyiwei self-collected 24 batches of corresponding medicinal materials, purchased 5 batches, a total of 29 batches (see Table 1), and was identified as Duyiwei Lamiophlomis rotata (Benth....

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Abstract

The invention provides a method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotate. In the detection method, the peak amount is large, separation degree is high, a base line is stable, the lamiophlomis rotate can be effectively detected, and new selection is provided for guaranteeing of medicine material quality. The iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients are detected under the same chromatographic condition for the first time, seven specific compounds are identified, and the quality of lamiophlomis rotate medicine materials can be comprehensively and accurately controlled.

Description

technical field [0001] The invention relates to a method for simultaneously detecting iridoid glycosides, phenylethanol glycosides, flavonoids, and caffeoyl components in Duyiwei. Background technique [0002] Lamiophlomis rotata (Benth.) Kudo is a plant of the genus Lamiophlomis rotata (Benth.) Kudo. It is an important medicinal plant on the Qinghai-Tibet Plateau and a commonly used bulk Tibetan medicine. It is a first-class endangered Tibetan medicine species [1] , "Four Medical Codes" and "Jingzhu Materia Medica" record that this product has the functions of strengthening muscles and bones, drying yellow water, and relieving pain. In modern clinical practice, it is used to promote blood circulation and stop bleeding, dispel wind and relieve pain, and widely treat bruises, rheumatic arthralgia, incision pain after surgery, bleeding and other diseases. It also has a good inhibitory effect on cancer pain and is non-addictive. The prospects are extremely bright. my country ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 古锐钟世红
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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