UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof

A fingerprint and detection method technology, applied in the field of UPLC fingerprint detection of unique medicinal materials and their preparations, can solve the problems of not using fingerprint quality control, etc., achieve simple pre-processing methods, save a lot of time, and improve detection efficiency Effect

Inactive Publication Date: 2019-07-05
JIUZHITANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there have been relevant reports on the content determination of the unique medicinal material and its preparations by UPLC technology, and the fingerprint quality control of the unique medicinal material and its preparations by HPLC technology. However, there is no UPLC method for the unique medicinal material and its preparations. Fingerprint quality control method, and in the prior art, up to 8 kinds of components in the unique medicinal material can be detected simultaneously. This method uses UPLC technology to retain as many active ingredients as possible in the unique medicinal material and its preparations as quickly as possible, and determines It is of great significance for the rapid identification of unique herbs and their preparations to establish standard spectra for 10 index components in unique herbs and 9 index components in unique preparations

Method used

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  • UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof
  • UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof
  • UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof

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Experimental program
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Embodiment 1

[0052] Example 1 The establishment of the fingerprint of unique medicinal material

[0053] 1. The source of medicinal materials

[0054] In order to make the medicinal materials sufficiently representative, medicinal materials from different origins or different harvest seasons were collected as test samples. See Table 3.

[0055] Table 3 Sources of unique flavor samples

[0056]

[0057] 2. Instruments and reagents, reagents

[0058] 2.1 Instrument: Waters H-class ultra-high performance liquid chromatography, PDA detector, Empower chromatographic workstation; Chromatographic column: Waters Acquity BEH C-18 column (2.1mm×150mm, 1.7μm, American Waters Company); water bath (Tianjin Test Instrument Co., Ltd.); Milli-Q pure water system (Millipore, USA); AY2.2 million electronic balance (Shimadzu, Japan).

[0059] 2.2 Reagents and reagents: methanol (AR, chromatographically pure, Sinopharm Chemical Reagent Co., Ltd.), ethanol (AR, Sinopharm Chemical Reagent Co., Ltd.), aceto...

Embodiment 2

[0074] Example 2 Establishment of Fingerprint of Duyiwei Preparation (Zhixue Zhitong Capsule)

[0075] 1. Source of preparation

[0076] In order to make the preparation sufficiently representative, 10 batches of samples of hemostasis and analgesic capsules produced by Chengdu Jiuzhitang Jinding Pharmaceutical Co., Ltd. in different months were collected as test samples. See Table 6.

[0077] Table 6 Sample batch numbers of Zhixue Analgesic Capsules

[0078]

[0079] 2. Instruments and reagents, reagents

[0080] 2.1 Instrument: Waters H-class ultra-high performance liquid chromatography, PDA detector, Empower chromatographic workstation; Chromatographic column: Waters Acquity BEH C-18 column (2.1mm×150mm, 1.7μm, American Waters Company); water bath (Tianjin Test Instrument Co., Ltd.); Milli-Q pure water system (Millipore, USA); AY2.2 million electronic balance (Shimadzu, Japan).

[0081] 2.2 Reagents and reagents: methanol (AR, chromatographically pure, Sinopharm Chemi...

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Abstract

The invention discloses a UPLC fingerprint spectrum detection method of lamiophlomis rotata (Benth.)Kudo and preparations thereof. The method includes the following steps of: (a) the preparation of areference substance solution; (b) the preparation of a test solution; (c) the provision of UPLC detection conditions; (d) the formation of a standard fingerprint spectrum with sesamoside, caffeic acid, chlorogenic acid, shanzhiside methyl ester, loganin, 8-O-acetyl shanzhiside methyl ester, forsythiaside B, galuteolin, verbascoside, Isoacteoside and the like adopted as reference peaks; and (e) thequality control of the fingerprint spectrum. According to the method, during test solution preparation, an extraction solvent is environment-friendly. The method is simple. Compared with a traditional HPLC method, the method is more convenient and rapider, and save energy and reduce energy consumption. The separation effect of the characteristic peaks of the fingerprint spectrum of the lamiophlomis rotata (Benth.)Kudo and the preparation thereof is good; and characteristic compositions and medicinal compositions can be reserved completely. The method has the advantages of good repeatability,high stability, high precision and strong specificity. The method can accurately and quickly control the lamiophlomis rotata (Benth.)Kudo and the preparations thereof.

Description

technical field [0001] The invention relates to the technical field of UPLC fingerprints of Chinese medicinal materials and preparations thereof, in particular to a detection method for UPLC fingerprints of unique medicinal materials and preparations thereof. Background technique [0002] UPLC technology is a technology based entirely on the basic principles of HPLC, with sub-two-micron small particles and ultra-high column efficiency packing as the core technology. At present, in the field of traditional Chinese medicine and Chinese patent medicine, the use of UPLC is becoming mature. Compared with HPLC, it not only retains all the advantages of high performance liquid chromatography, but also has the unique advantages of saving time, energy and environmental protection. [0003] The unique medicinal material is the dry aerial part of Lamiophlomis Rotata (Benth.) Kudo, a plant of the Labiatae family. It is a commonly used medicine for hemostasis and analgesia by Tibetans, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 邓芸黄胜叶惠煊周良袁利群
Owner JIUZHITANG
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