Method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotata
A technology of iridoid glycosides and phenylethanol glycosides, which is applied in the fields of phenylethanol glycosides and simultaneous detection of iridoid glycosides, caffeoyl components, and flavonoids in the unique flavor, achieving accurate quality control, stable baseline, and effective detection effects
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Embodiment 1
[0023] Embodiment 1 Detection method of the present invention
[0024] (1) Take the single medicinal material to be tested, extract it with 60% v / v methanol, and prepare the test solution;
[0025] (2) Select shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester, chlorogenic acid, forsythiaside B, ergoside, rutin and luteolin as reference substances, and prepare reference substance solutions;
[0026] (3) Inject the reference substance and the test solution into the high-performance liquid chromatograph respectively, and detect the above-mentioned 7 components in Duyiwei according to the external standard method, and the chromatographic conditions are as follows:
[0027] Chromatographic column: Octadecyl bonded silica gel as filler
[0028] Detection wavelength: the detection wavelength of shanzhiside methyl ester and 8-O-acetyl shanzhiside methyl ester is 238nm; the detection wavelength of chlorogenic acid, forsythiaside B and ergoside is 330nm; the detection wavele...
Embodiment 2
[0031] Embodiment 2 Methodological investigation
[0032] 1 material
[0033] Utimate 3000 high performance liquid chromatography (including quaternary gradient pump, online degasser, autosampler, column thermostat, VWD3400 UV detector, Chromeleon chromatography workstation, Diana, Germany), Sartorius BS224S electronic analytical balance (Germany Doris), MILLIQ ultrapure water machine (Millipore, USA)
[0034] Shanzhiside methyl ester, chlorogenic acid, 8-O-acetylshanzhiside methyl ester, forsythiaside B, rutin, ergosteroside, and luteolin reference substances were all purchased from Chengdu Master Biotechnology Co., Ltd. (for content determination, purity ≥ 98%). Acetonitrile (chromatographically pure, Fisher), water was ultrapure water, and other reagents were analytically pure.
[0035] Duyiwei self-collected 24 batches of corresponding medicinal materials, purchased 5 batches, a total of 29 batches (see Table 1), and was identified as Duyiwei Lamiophlomis rotata (Benth....
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