Construction method of hplc characteristic map of a kind of Chinese patent medicine "Qingyi Lidan Granule"

A technology for clearing the pancreas, promoting gallbladder, and characteristic spectrum, which is applied in the field of construction of HPLC characteristic spectrum of Chinese patent medicines, can solve problems such as difficult product quality, and achieve the effects of easy identification, high similarity and high precision.

Active Publication Date: 2022-07-22
抚松县中药有限责任公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Its quality standards only control the qualitative and quantitative detection methods of blind medicines, and it is difficult to control the product quality as a whole

Method used

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  • Construction method of hplc characteristic map of a kind of Chinese patent medicine "Qingyi Lidan Granule"
  • Construction method of hplc characteristic map of a kind of Chinese patent medicine "Qingyi Lidan Granule"
  • Construction method of hplc characteristic map of a kind of Chinese patent medicine "Qingyi Lidan Granule"

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: A kind of HPLC characteristic chromatogram construction method of Qingyilidan granules

[0030] Instrument: Agilent 1200 high performance liquid chromatograph, MS205DU analytical balance

[0031] Reagents: Paeoniflorin reference substance, chlorogenic acid reference substance, acetonitrile for liquid chromatographic analysis were chromatographically pure, the rest of the reagents were analytically pure, water was ultrapure water, and Qingyilidan granules were provided by Fusong County Traditional Chinese Medicine Co., Ltd. .

[0032] Preparation of the test solution: take 0.5g of Qingyilidan granule fine powder, accurately weigh it, place it in a stoppered conical flask, accurately add 20ml of methanol, weigh it, ultrasonically treat it for 20 minutes, let it cool, and weigh it again. Determine the weight, use methanol to make up the lost weight, shake well, filter, and take the continuous filtrate.

[0033] Preparation of reference substance solution: r...

Embodiment 2

[0038] Embodiment 2: A kind of HPLC characteristic chromatogram construction method of Qingyilidan granules

[0039] Instrument: Agilent 1200 high performance liquid chromatograph, MS205DU analytical balance

[0040] Reagents: Paeoniflorin reference substance, chlorogenic acid reference substance, acetonitrile for liquid chromatographic analysis were chromatographically pure, the rest of the reagents were analytically pure, water was ultrapure water, and Qingyilidan granules were provided by Fusong County Traditional Chinese Medicine Co., Ltd. .

[0041] Preparation of the test solution: take 1.5g of Qingyilidan granule fine powder, accurately weigh it, place it in a conical flask with stopper, accurately add 30ml of methanol, weigh it, ultrasonically treat it for 40 minutes, let it cool, and weigh again. Determine the weight, use methanol to make up the lost weight, shake well, filter, and take the continuous filtrate.

[0042] Preparation of reference substance solution: r...

Embodiment 3

[0048] Embodiment 3: a kind of HPLC characteristic chromatogram construction method of Qingyilidan granules

[0049] Instrument: Dionex U-3000 high performance liquid chromatograph, MS205DU analytical balance

[0050] Reagents: Paeoniflorin reference substance, chlorogenic acid reference substance, acetonitrile for liquid chromatographic analysis were chromatographically pure, the rest of the reagents were analytically pure, water was ultrapure water, and Qingyilidan granules were provided by Fusong County Traditional Chinese Medicine Co., Ltd. .

[0051] Preparation of the test solution: take 1.0 g of Qingyilidan granule fine powder, accurately weigh it, place it in a conical flask with a stopper, add 25 ml of methanol precisely, weigh it, ultrasonically treat it for 30 minutes, let it cool, and weigh it again. Determine the weight, use methanol to make up the lost weight, shake well, filter, and take the continuous filtrate.

[0052] Preparation of reference substance solu...

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Abstract

A method for constructing a HPLC characteristic spectrum of a Chinese patent medicine "Qingyi Lidan Granule" belongs to the technical field of traditional Chinese medicine, and the method comprises: (1) preparation of a test solution; (2) preparation of a reference solution; (3) chromatography Conditions: Agilent ZORBAX SB‑C18 (5μm, 4.6×250mm) column, mobile phase A is acetonitrile, mobile phase B is 0.2% phosphoric acid solution, gradient elution, flow rate 0.9~1.1ml / min, column temperature 30~ 40℃, the detection wavelength is 235~245nm. The theoretical plate number should not be less than 3000 according to the paeoniflorin peak. (4) Determination: Determination of characteristic spectrum according to high performance liquid chromatography. By establishing the HPLC characteristic chromatogram of 10 batches of Qingyi Lidan granules, 10 characteristic peaks were determined, and the standard characteristic chromatogram of Qingyi Lidan granules was established. The invention has the characteristics of convenience, speed, stability, high precision, good reproducibility and the like, and can effectively control the quality of the Qingyilidan granules.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for constructing an HPLC characteristic map of traditional Chinese medicines. Background technique [0002] Qingyi Lidan Granules (composed of eight medicinal materials of oyster, turmeric, honeysuckle, Bupleurum, rhubarb, vinegar Corydalis, peony bark, red peony root.) The standard is included in the national drug standard, standard number: YBZ01052015. The quality standard of this product includes the properties of the granules; the thin layer identification of rhubarb, honeysuckle and vinegar Corydalis; the content determination of paeoniflorin. Its quality standard only controls the qualitative and quantitative detection methods of blind medicine, and it is difficult to control the product quality as a whole and comprehensively. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to provide a method for constr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 田淋淋徐建白冰王伟张显涛康金龙
Owner 抚松县中药有限责任公司
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