Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HPLC fingerprint identification method for origin ginseng protection

A fingerprint, origin technology, applied in the field of HPLC fingerprint identification, to achieve the effect of simple pretreatment method, good stability and good reproducibility

Inactive Publication Date: 2009-04-29
HEBEI UNIVERSITY
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic research on the characteristic identification factors of high-end health products ginseng is still in the development stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC fingerprint identification method for origin ginseng protection
  • HPLC fingerprint identification method for origin ginseng protection
  • HPLC fingerprint identification method for origin ginseng protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] 1. Establish the liquid chromatography fingerprint of ginseng in the place of origin

[0052] A. Establish the liquid chromatography fingerprint of ginseng in the place of origin

[0053] 1. Preparation of reference solution

[0054] Accurately weigh an appropriate amount of ginsenoside Re reference substance, add methanol to dissolve and make a 1.00 mg / mL reference substance solution.

[0055] 2. Preparation of standard solution

[0056] Put commercially available Changbai Mountain ginseng as a standard product in a drying oven at 60°C for 4 hours and heat to constant weight, crush and sieve, and collect powder samples below 80 mesh;

[0057] Accurately weigh 2.0g Changbai Mountain ginseng powder sample and add it to the automatic temperature-controlled solvent reflux liquid-solid extractor, add 100mL methanol for extraction for 5 hours, extract the solution by rotary evaporation to recover the solvent, and pour the concentrated solution into a 10mL volumetric flask;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for identifying HPLC fingerprints for protecting ginseng of origin, which is characterized by comprising the following steps: A, establishing liquid phase chromatograph fingerprints of the ginseng of origin, namely using the ginseng in the same habitat as a standard, analyzing the ginseng by liquid phase chromatograph and establishing fingerprints; and B, detecting a sample, namely taking a ginseng sample to be detected, detecting the sample under the same conditions as the standard respectively to obtain a spectrogram of the detected sample, and analyzing the fingerprints of the detected sample and the standard by a direct observational method or fingerprints software as qualitative basis. The method has simple pretreatment for fingerprints of the ginseng medicinal material established by adopting the liquid phase chromatograph, multiple peak numbers and better separation degree, therefore, contents of main chemical compositions of the ginseng medicinal material have large difference caused by different growing geographic environments and climates. The HPLC fingerprints of the ginseng can provide reference for distinguishing high-end ginseng health-care products from origin places and non-origin places, and provide reference for establishing fingerprints of other traditional Chinese medicinal materials. The research of methodology shows that all the precision, the stability and the repeatability have better application prospect.

Description

technical field [0001] The invention relates to an HPLC fingerprint identification method used for the protection of ginseng in the place of origin, and belongs to the technical field of liquid chromatography analysis. Background technique [0002] Due to the rich nutritional value and pharmacological effects of ginseng, ginseng medicinal materials have always attracted much attention. In foreign markets, there have been many high-end health foods and health medicines made from ginseng medicinal extracts. Ginseng medicinal materials have also become one of the best-selling botanical preparations in the natural medicine market in Europe and America. However, ginseng from different origins has different components due to the influence of environmental climate, water and soil conditions, which leads to differences in clinical efficacy. Therefore, many unscrupulous traders use some non-origin ginseng as shoddy products, confusing the real ones with fake ones, which greatly end...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 闫正苑若瑶张智慧于趁王春云蔡立鹏
Owner HEBEI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com