Method for detecting three kinds of flavonoid constituents in seedless roxburgh rose fruits simultaneously

Abstract

The invention discloses a method for detecting three kinds of flavonoid constituents in seedless roxburgh rose fruits simultaneously. The method comprises the steps that seedless roxburgh rose fruit dry powder to be detected is obtained, methyl alcohol or water-containing methyl alcohol is used for extraction, filtrate decompression and rotary drying are carried out, ultrapure water is added, normal butanol is saturated with water, extraction is carried out two times according to the ethanol and water ratio of 4:1, then, extraction is carried out two times through ethyl acetate, filtrates are combined and subjected to decompression and rotary drying, the volume is made constant, and a test solution is prepared; rutin, quercetin and kaempferol are selected as comparison products for preparing a comparison solution; the comparison solution and the test solution are sample and analyzed in a high performance liquid chromatograph under the same chromatographic condition, and the three kinds of flavonoid constituents in seedless roxburgh rose fruits are monitored according an external standard method. The main flavonoid constituents in seedless roxburgh rose fruits are detected for the first time under the same chromatographic condition, three specific compounds are identified, and the method can be more comprehensively and accurately used for intensive study of flavonoid active constituents in seedless roxburgh rose fruits.

Description

technical field [0001] The invention relates to a method for detecting flavonoid components, in particular to a method for detecting three kinds of flavonoid components in the fruit of Rosa roxburghii. Background technique [0002] Seedless prickly pear ( Rosa sterilis S.D.Shi) is a perennial climbing fruit tree belonging to the family Rosaceae Rosa. It is a unique species in Guizhou. area is of great importance. Its ecological value is mainly reflected in rocky desertification control, water and soil conservation, and ecological restoration. The medicinal and health value is mainly reflected in the gradual launch of seedless Rosa roxburghii medicine and health care products, with a good market prospect. [0003] Studies have shown that the content of flavonoids in the bioactive components of Rosa roxburghii is the highest. Flavonoids are small molecular compounds, which are widely used as useful ingredients in many Chinese herbal medicines, and have good antioxidant and...

AI Technical Summary

Problems solved by technology

However, as far as the current research status is concerned, there are few reports on the quality control of Rosa roxburghii, and there are even fewer studies on the determination of certain components in it by HPLC. Rutin and gallic acid in pear fruit

There is no report on the simultaneous determination of three or more components in Rosa roxburghii by HPLC

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