The invention discloses a preparation method of rosa-roxburghii wine and belongs to the technical field of wine brewing. The preparation method comprises the following steps: crushing and juicing fresh rosa-roxburghii fruits, then adding activated pectinase into the juice, circularly stirring to be uniform, then dipping, then activating active dry yeast, and then pouring rosa-roxburghii juice and the activated active dry yeast into a fermentation tank; after fermenting for a period of time, pouring fermented materials into another fermentation tank, simultaneously removing precipitates, then adding grape yeast, adding honey to adjust the sugar, and after stirring to be uniform, fermenting; after the fermentation is finished, emptying raw wine from the bottom of the fermentation tank, carrying out tank exchange and filtering, standing and canning by using a bottle, and thus obtaining the rosa-roxburghii wine. The rosa-roxburghii wine disclosed by the invention is pure and sweet in mouthfeel, is mellow, full and tasty, is long in aftertaste, and has the effects of clearing heat, promoting diuresis, resisting senility, resisting cancer, invigorating the stomach and promoting digestion.