HPLC fingerprint detection method for balsam pear leaves
A detection method and fingerprint technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of evaluating the real quality of traditional Chinese medicines, easy to influence each other, etc., and achieve the effects of good separation, quality assurance of medicinal materials, and high precision.
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Embodiment 1
[0051] The screening of embodiment 1 process parameter
[0052] 1. Screening of chromatographic conditions
[0053] (1) Inspection of detection wavelength
[0054] Take (No. 7 sample in Table 1) balsam pear leaf medicinal material powder 2.0g, accurately weigh, place in a 250mL conical flask, add 50mL of 50% methanol, weigh, extract by ultrasonic for 30min, cool, make up to the original volume with 50% methanol Weight, filtered through a 0.45 μm microporous membrane to obtain the test solution. Aspirate 10 μl, and record the chromatogram of the test solution under full-wavelength scanning. By comparing the 3D chromatograms, it was found that the peaks at 250nm had stronger absorption and better resolution, so the detection wavelength was set at 250nm.
[0055] (2) Chromatographic conditions
[0056] Take 20 μl of the test solution, inject it into a high-performance liquid chromatograph, and record the chromatograms in which the mobile phase is acetonitrile-water and methan...
Embodiment 2
[0081] Embodiment 2 The method of the present invention and its methodology verification
[0082] 1. Establishment of HPLC control fingerprints of medicinal materials
[0083] (1) HPLC fingerprints and control fingerprints of bitter gourd leaves from different origins
[0084] Get 10 batches of balsam pear leaves medical material in table 1, prepare need testing solution according to the preparation method of need testing solution under embodiment 1, and experiment under the chromatographic conditions under embodiment 1, record HPLC chromatogram.
[0085] The results show: the HPLC chromatogram is about No. 2 peak of retention time 32.431min as reference peak (S), and its retention time and peak area are 1, and the RSD of the relative retention time of each common peak is all less than 1.5%, and relative peak area The RSDs of the balsam pear leaves are all relatively large, and the similarity of the chromatograms is calculated with the "Chinese Medicine Chromatographic Finger...
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