HPLC fingerprint detection method for balsam pear leaves

A detection method and fingerprint technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of evaluating the real quality of traditional Chinese medicines, easy to influence each other, etc., and achieve the effects of good separation, quality assurance of medicinal materials, and high precision.

Inactive Publication Date: 2017-01-11
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Considering that there are many components in Chinese medicinal materials, and each component is easy to interact with each other, it is difficult to fully evaluate the true quality of traditional Chinese medicine with the content of only one or two active ingredients.

Method used

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  • HPLC fingerprint detection method for balsam pear leaves
  • HPLC fingerprint detection method for balsam pear leaves
  • HPLC fingerprint detection method for balsam pear leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The screening of embodiment 1 process parameter

[0052] 1. Screening of chromatographic conditions

[0053] (1) Inspection of detection wavelength

[0054] Take (No. 7 sample in Table 1) balsam pear leaf medicinal material powder 2.0g, accurately weigh, place in a 250mL conical flask, add 50mL of 50% methanol, weigh, extract by ultrasonic for 30min, cool, make up to the original volume with 50% methanol Weight, filtered through a 0.45 μm microporous membrane to obtain the test solution. Aspirate 10 μl, and record the chromatogram of the test solution under full-wavelength scanning. By comparing the 3D chromatograms, it was found that the peaks at 250nm had stronger absorption and better resolution, so the detection wavelength was set at 250nm.

[0055] (2) Chromatographic conditions

[0056] Take 20 μl of the test solution, inject it into a high-performance liquid chromatograph, and record the chromatograms in which the mobile phase is acetonitrile-water and methan...

Embodiment 2

[0081] Embodiment 2 The method of the present invention and its methodology verification

[0082] 1. Establishment of HPLC control fingerprints of medicinal materials

[0083] (1) HPLC fingerprints and control fingerprints of bitter gourd leaves from different origins

[0084] Get 10 batches of balsam pear leaves medical material in table 1, prepare need testing solution according to the preparation method of need testing solution under embodiment 1, and experiment under the chromatographic conditions under embodiment 1, record HPLC chromatogram.

[0085] The results show: the HPLC chromatogram is about No. 2 peak of retention time 32.431min as reference peak (S), and its retention time and peak area are 1, and the RSD of the relative retention time of each common peak is all less than 1.5%, and relative peak area The RSDs of the balsam pear leaves are all relatively large, and the similarity of the chromatograms is calculated with the "Chinese Medicine Chromatographic Finger...

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Abstract

The invention discloses an HPLC fingerprint detection method for balsam pear leaves. The method comprises the steps of preparing a contrast product solution, preparing a reference product solution, establishing a contrast fingerprint spectrum, and preparing and detecting a test product solution. By virtue of screening of multiple processes, multiple components in the balsam pear leaves are effectively separated and detected, the obtained fingerprint spectrum has multiple peaks, the separation degree of the peaks is good, and the method is good in repeatability and high in precision, so that the quality of a balsam pear leaf medicinal material can be monitored easily, conveniently, quickly, comprehensively, accurately and reliably, and the effective guarantee is provided for comprehensively monitoring the quality of the balsam pear leaf medicinal material.

Description

technical field [0001] The invention relates to the technical field of medicine quality control methods, in particular to an HPLC fingerprint detection method of bitter melon leaves. Background technique [0002] Bitter melon leaves are the dried leaves of the annual vine herb Momordica charantia L. of the family Cucurbitaceae. Cool in nature, bitter in the mouth. It has the effects of clearing heat and detoxifying, sterilizing, stopping dysentery and relieving pain, and can treat stomach pain, dysentery, boils and swollen toxins. As a common folk medicine, bitter gourd leaves are widely used. They are often used to treat redness, swelling and heat pain of the skin after mosquito bites, prickly heat in children, heat ulcers, red bayberry sores, dog bites and goosefoot. [0003] Bitter gourd leaves are rich in resources, have definite curative effects, are widely used by folks, are highly recognized, are convenient and easy to obtain, and have good development value. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蒋桂华于宗强连艳兰群刘晓芬王潇霖郑潇潇黄凤
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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