61results about How to "The method is reproducible" patented technology

The invention provides a method for measuring contents of coumarin and safrole in flavor and fragrance for cigarette, which comprises the steps of: first, dispersing coumarin and safrole in flavor and fragrance for cigarette in an aqueous solution and extracting with an organic reagent, sampling, filtering and performing instrument analysis and content measurement by using gas chromatography-mass spectrometry; selecting phenylethyl propionate as an internal standard substance and selecting ions of phenylethyl propionate, coumarin and safrole with mass to charge ratios m/z which are respectively 104, 146 and 162 in the mass spectrogram for quantitative analysis; and drawing a standard curve by using an internal standard method and fitting the sample with the standard curve to obtain the contents of coumarin and safrole. By adopting the method, coumarin and safrole in flavor and fragrance for cigarette are more completely extracted; and in the process of extraction, interfering materials can be removed to an extreme extent so that the sample spectrum is purified and pollution of samples to the injection port of the instrument and the chromatographic column is reduced, thereby the demand of sample detection in batches in actual work is met.

The invention relates to a transition metal based catalyst electrode of a honeycomb macroporous structure as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: S1, synthesizing a polystyrene sub-microsphere aqueous solution; S2, preparing a transition metal salt solution, mixing the transition metal salt solution and the polystyrene sub-microsphere aqueous solution to obtain a mixed solution, spraying the mixed solution onto a conductive substrate for forming a film, and calcining at a high temperature so as to obtain an oxide film with a honeycomb macroporous structure; and S3, performing sulfidizing or selenizing or phosphating treatment on the oxide film at the high temperature in an inert atmosphere, thereby obtaining the transition metal based catalyst electrode of the honeycomb macroporous structure. The preparation method disclosed by the invention has the advantages that the operation is simple, the macroporous structure of the catalyst is easily maintained, the reproducibility is high, and a large-area electrode is conveniently prepared. Moreover, the catalyst electrode prepared by the invention has high specific surface area and capable of exposing more active sites and can be applied to dye-sensitized solar cells, electrochemical decomposition of water and the like.

The invention discloses a preparation method of a porous cobaltosic oxide nanosheet. By utilizing plant straws as a carbon source, so that the raw material source is wide, and the cost is low; the porous cobaltosic oxide nanosheet is prepared by virtue of an impregnation method and a secondary roasting method, the prepared porous cobaltosic oxide nanosheet is formed by Co3O4 nanocrystals with a good crystallization property, and the particle sizes of the Co3O4 nanocrystals are 5nm-20nm, so that the nanosheet has a relatively large specific surface area and rich pore structures. The porous cobaltosic oxide nanosheet has the advantages of high volume and long service life when being applied to supercapacitors.

The invention discloses a preparation method of a zigzag Cu2MoS4 nanosheet electrocatalyst and photocatalyt. The method is characterized by including the steps of synthesizing a Cu2O precursor and synthesizing a zigzag Cu2MoS4 nanosheet. The method has the advantages that the raw materials are abundant in source, the route is green, the method is good in repeatability, and large-scale synthesis can be achieved. The prepared material can be used for electrocatalysis hydrogen production and photocatalytic degradation. The reaction is efficient, operation is easy, the prepared material is high in stability, and good practical value and application prospects are achieved.

The invention relates to a method and a device for measuring the crushing degree of tobacco shreds. By the established crushing degree measuring method, not only the capability and the level of a material incoming end working procedure can be represented, but also the mass variation of the crushed tobacco shreds can be reflected through a crushing process, so that the physical quality of final cigarette products can be predicted. By utilizing the method and the device, the crushing of the tobacco shreds in any process can be studied, the self anti-crushing property of raw materials can be reflected, and the influence of external force factors, such as process flows, conditions and the like on the anti-crushing property thereof can be studied. The invention has the following advantages: by directly measuring the crushing degree of the tobacco shreds, the anti-crushing ability of the tobacco shreds is represented correctly and the size distribution conditions of the tobacco shreds before being crushed and after being crushed are reflected; the invention is of great significance in predicting the processing quality of the tobacco shreds and provides an effective measure for studying the crushing process of tobacco shreds; meanwhile, the invention has advantages of low sampling quantity, simple operation and good method reproducibility. The designed special crushing device has reasonable structure and can simulate the crushing degree of any processing on the tobacco shreds.

The invention discloses a detection method of a nine-component heart-calming particle. The detection method of the nine-component heart-calming particle can carry out thin-layer chromatography on spina date seed, schisandra chinensis, corydalis tuber, cinnamon, asparagus fern and polygala tenuifolia in the nine-component heart-calming particle, microscopic identification on calcium oxalate in ginseng and determine HPLC (high performance liquid chromatography) content of ginsenoside Rg1 and ginsenoside Re. The detection method of the nine-component heart-calming particle has the characteristics of stability, high precision, good reproducibility, high recovery ratio and good separation effect. By adopting the detection method of the nine-component heart-calming particle, the quality of the nine-component heart-calming particle can be further comprehensively controlled, the provided method for determining the content of ginsenoside Rg1 and ginsenoside Re can realize good baseline separation effect on ginsenoside Rg1 and ginsenoside Re, and a determination result is accurate and the reproducibility is good.

The invention provides a liquid phase micro-extraction device for complex matrix sample on-line pretreatment of a capillary electrophoresis-ampere detector as well as a usage method thereof, a liquid phase micro-pumping device of the device is fixed on a fixed mount, the lower end of the liquid phase micro-pumping device is nested with a section of U-shaped hollow fiber pipe, and a capillary sample inlet end and an electrode are inserted into the other end of the hollow fiber pipe, the U-shaped hollow fiber pipe is positioned in a sample container, and a stirring device is also arranged on the sample container; and the liquid phase micro-extraction in the complex matrix sample pretreatment can be implemented according to the device of the invention. The invention has a complex matrix sample liquid phase micro-extraction pretreatment device, a capillary electrophoresis device and an ampere detector connected together to form an integral device, the range of detected substances is extended, and the sensitivity, reproducibility, stability and reliability for separating capillary electrophoresis are improved; and the device has advantages of small volume, simple structure, simple operating method, low cost, good reproducibility of the method, and convenient sample analysis after pretreatment.

The invention belongs to the technical field of water-borne wood paint authentication, and particularly relates to a method for authenticating the truth or falsity of polyurethane water-borne wood paint on the basis of a nonlinear fingerprint graph. A water-borne wood paint sample is subjected to preliminary treatment to quicken the layering of sample emulsion, so that an organic phase and an inorganic phase are fully separated, and an emulsion breaking effect on samples is guaranteed; the method is convenient for subsequent detection; if acrylic acid water-borne wood paint is doped in the polyurethane water-borne wood paint, during detection, acrylic acid emulsion subjected to acidolysis in sulfuric acid solution generates acrylic acid with reducing properties; and the above products aremixed in a B-Z vibration system consisting of H+, BrO3, Mn2+ and acrylic acid, a sample to be authenticated and a standard sample are compared according to the extraction characteristic parameters ofthe nonlinear fingerprint graph, and the variety as well as the truth or falsity of the sample to be authenticated can be authenticated according to an integral similarity. In addition, the method hasthe advantages of being good in reproducibility, short in operation time, economic and the like, and is suitable to be widely prompted and applied in authentication fields of the polyurethane water-borne wood paint.

The invention discloses an extracting separating method of flavone from rheum officinale Cynanchum, which is characterized by the following: affirming extracting condition through alcohol; setting the extracting quantity of ether and water stature butanol; comparing the relationship of flavone structure with silica gel thin-layer chromatography, gluglucosan gel thin-layer chromatography and polyamide thin-layer chromatography; affirming the approximate flavone compound to high-effective chromatographic separating purifying structure.

The invention discloses a method for determining microcystin MC-LR in a water body, which belongs to the technical field of environmental protection. The method for determining microcystin MC-LR in the water body utilizes glass fiber (GF)/ C glass fiber filter paper to filter frustules in a water sample to be determined, and a solid-phase extraction column-SPE column which takes C18 silica gel as a filler to separate and purify the filtered water sample to be determined, a high performance liquid chromatography is used to detect, and then, the pre-obtained standard curves of the corresponding peak areas of microcystin MC-LR standard substances with different concentrations is utilized to calculate the content of the microcystin MC-LR in the water sample to be determined. The method for determining microcystin MC-LR in the water body has the advantages of simplicity in steps, convenience in operation, reduction in eluant usage and environmental toxicity, safety in operating personnel, low detection cost and the like, and the minimum detection limit is 0.002mg/L.

The invention belongs to the field of analysis on difficulty-smelted compounds and discloses an analysis detection method of an iron element in vanadium carbide. The analysis method comprises the following analysis steps of: heating a sample by a nitric acid water solution to be dissolved; carrying out dry filtering on free carbon of an insoluble substance in the sample; preparing an iron standard solution and utilizing a substrate matching method to eliminate the inference on iron by vanadium; utilizing an atom absorption spectroscopy to measure the absorption value of an iron-based state atom; and calculating an analysis result according to a standard curve. The analysis detection method of the iron element in the vanadium carbide has the advantages of high sensitivity, simplicity and convenience for operation, and good reproducibility of the analysis result, and is suitable for measuring the iron content in the vanadium carbide.

The invention discloses a detection method for contents of varieties of flavonoids in taxus chinensis branches. The detection method is used for detecting contents of rutin, quercetin, amentoflavone,isoginkgetin and sciadopitysin and comprises the following steps: preparing a test sample solution; preparing a control product solution; performing high performance liquid chromatography measurementand respectively injecting the test sample solution and the control product solution into a high performance liquid chromatograph to be detected, wherein the chromatographic conditions are as following: C18 is utilized as a chromatographic column, a methyl alcohol-water mixed solution is utilized as a flow phase, gradient elution is performed, detection wavelength is 330nm, a flow velocity is 1.0mL/min, a column temperature is 30 DEG C, and the number of theoretical plates is not lower than 3000. The detection method disclosed by the invention utilizes the high performance liquid chromatography to detect contents of the rutin, the quercetin, the amentoflavone, the isoginkgetin and the sciadopitysin in the taxus chinensis branches at the same time; the method has the advantages of simpleness, good repeatability, high recovery rate, ability in accurately measuring the contents of 5 kinds of flavonoids in the taxus chinensis branches, convenience and quickness.

The invention discloses a baobab sterile germination accelerating method and a germination accelerating culture medium. The method includes: using concentrates sulfuric acid for acid etching, using aliquid detergent for flushing, using flowing water for flushing, using alcohol for soaking, using sterile water for flushing, using a HgCi2 solution for soaking, using sterile water for cleaning, andculturing a germination accelerating culture medium, wherein the germination accelerating culture medium contains cymbopogon distans extract, lemon grass extract, garlic extract, betaine and indolebutyric acid. The defect of high-degree dormancy of baobab seeds is overcome; by the method, baobab seed germination rate is up to higher than 90%, transplanting survival rate is up to higher than 88%, baobab seedlings are uniform in growing vigor, and the method is high in repeatability. The seed germination rate and the transplanting survival rate of baobab are increased greatly, a lot of baobab seedlings with uniform growing vigor can be provided within a short time, and the method is of great significance in baobab production.

The invention discloses a method for detecting moisture content of explosion beads for cigarettes. According to the method, explosion bead samples for cigarettes are pretreated, separated by a capillary column and analyzed by a gas chromatography-thermal conductivity detector method; each sample is measured twice in parallel; in each experiment, a blank sample is needed; and quantification is carried out by adoption of an internal standard method so as to obtain moisture contents in the beads. The detection method is verified to have an adding standard recovery rate of 98.95%-104.48%, a methodquantification limit of 0.0577 mg/mL, an intraday precision of 3.47% and a day-to-day precision of 4.05%. The method is simple in pre-treatment operation and good in reproducibility, and is capable of correctly analyzing and quantifying explosion bead samples with low moisture contents.

The invention relates to a method for detecting N, N'-diacetyl-L-cystine in a compound amino acid injection by applying high performance liquid chromatography. The method comprises the following steps: preparing a mobile phase, preparing a reference substance solution, preparing a test solution, setting chromatographic instrument parameters, detecting the reference substance solution, detecting the test solution, and calculating the content of N, N'-diacetyl-L-cystine in the test solution. According to the method, a phosphate mobile phase system is adopted, separation of N, N'-diacetyl-L-cystine and adjacent chromatographic peaks can be achieved on a common C18 chromatographic column, operation is easy and convenient, the method is easy to implement, and compared with an ion pair mobile phase system, the method is good in reproducibility, does not need expensive ion pair reagents and is more economical.

The invention discloses a method for separating and purifying antibacterial components in cumin essential oil and belongs to the field of separation and extraction of natural products. The method includes the steps that firstly, petroleum ether is used as a solvent to carry out digestion on cumin, and the cumin essential oil is obtained; the cumin essential oil is sequentially separated into saturated hydrocarbon, aromatic hydrocarbon, nonhydrocarbon and colloid through column chromatography; the effective antibacterial components in the essential oil are obtained. Through verification, the extracted cumin essential oil components are remarkable in antibacterial and bacteriostatic effect, and extract from cumin plants can serve as a natural food preservative and is safe and reliable.

The invention belongs to the technical field of medicine, and specifically relates to a crystal form of pazopanib hydrochloride and a preparation method thereof. The new crystal form of pazopanib hydrochloride obtained in the present invention has the advantages of high purity, the largest impurity is less than 0.5‰, and stability Good performance; the method has good reproducibility, and the purity and crystal form can be well reproduced when it is scaled up to the pilot scale.

The invention relates to a traditional Chinese medicinal material and a method for detecting mass of a traditional Chinese medicinal material preparation, and concretely relates to a centella asiaticamedicinal material and an extract product and the method for detecting the mass of the preparation. The method employs a high performance liquid chromatography for separating, through a QAMS technology, centella asiatica is taken as an internal standard substance, a relative correction factor of centella asiatica, madecassoside and asiaticoside-B is established, and the content of madecassoside and asiaticoside-B can be calculated. The method has the advantages of high detection sensitivity and good stability, can be objectively, comprehensively and accurately evaluate the mass of the centella asiatica medicinal material and its extract product and its preparation, can solve the problems of non objective and reasonable control of the medicinal material and the preparation mass due to lacking of a contrast product, and has important meaning for controlling mass and guaranteeing curative effect.

The invention discloses a Chinese buckeye seed decoction piece preparation method. The preparation method includes: fumigating Chinese buckeye seeds by 10-40% lactic acid water solution, peeling the Chinese buckeye seeds and slicing the peeled Chinese buckeye seeds; subjecting the slices to microwave drying, ultraviolet sterilization and the like. A Chinese buckeye seed decoction piece prepared by the preparation method has the advantages that turnover rate of each of aescine A and aescine B is above 90%, and the aescine A and the aescine B are not affected by washing time, drying temperature and drying time. The preparation method is good in repeatability, and product quality is more controllable.