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Electrochemical fingerprint identification of traditional Chinese medicine pawpaw

A fingerprint and electrochemical technology, applied in the field of electrochemical fingerprint identification of papaya, can solve the problem of not establishing a papaya fingerprint, and achieve the effects of fast and economical operation, good reproducibility, and easy operation.

Inactive Publication Date: 2015-11-18
查孝柱
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the papaya fingerprints that have been established in China are mainly HPLC fingerprints. The index components of the research focus on organic acids and triterpenes. No fingerprints that reflect the characteristics of all chemical components of papaya have been established. Electrochemical fingerprints The object of detection is all chemical components in Chinese herbal medicines

Method used

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  • Electrochemical fingerprint identification of traditional Chinese medicine pawpaw
  • Electrochemical fingerprint identification of traditional Chinese medicine pawpaw

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Experimental program
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Effect test

Embodiment Construction

[0022] 1. Establishment of electrochemical fingerprints of the chemical components of papaya:

[0023] Using 7 batches of Jusheng brand papaya as a standard, the electrochemical fingerprints of its chemical composition were established;

[0024] Instruments LK2005A electrochemical workstation, HH-501 super constant temperature water bath, 85-2A digital constant temperature magnetic stirrer, 213 metal platinum electrode, 217 calomel electrode;

[0025] The chemical oscillation reaction was carried out in a jacketed reactor with continuous stirring. Turn on the super constant temperature water bath, adjust the temperature at (310±0.05)K, add 0.50g papaya, 2.0mLCO(CH 3 ) 2 Solution, 12mL3.0mol / LH 2 SO 4 Solution, 6mL0.4mol / LCH 2 (COOH) 2 Solution, 3mL0.005mol / LCe(SO 4 ) 2 The solution was placed in a jacketed reactor, and the reactor lid was covered with a thermometer, a 213-type metal platinum electrode and a 217-type calomel electrode. Turn on the computer connected to ...

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Abstract

The present invention relates to a pawpaw electrochemical fingerprint identification method, provides a method for identifying the authenticity of pawpaw through an electrochemical fingerprint, and belongs to the field of traditional Chinese medicine quality control. The method comprises: A, establishing a pawpaw chemical component fingerprint, wherein pawpaw with the same production place and the same brand is adopted as a standard substance, the electrochemical fingerprint of all component is established through an oscillation reaction, and results are shown in figure 1; and B, detecting a sample, wherein a pawpaw sample to be detected is detected under the same conditions as the standard substance so as to obtain the fingerprint of the detected sample, and the fingerprints of the standard substance and the detected sample are analyzed through a direct observation method or Origin software analysis so as to be adopted as the qualitative basis. According to the present invention, all components of the pawpaw are made into the fingerprint, the method has advantages of good reproducibility, no requirement of pretreatment, simple and fast operation, economy and the like, can provide reliable information for the identification of the quality of all types of pawpaw and the identification of the low-price pawpaw and the high-price pawpaw, and is suitable for promotion in the technical field of pawpaw safety detection.

Description

technical field [0001] The invention relates to an electrochemical fingerprint method for identifying papaya, and belongs to the technical field: 2. Biology and new medical technology, (2) Chinese medicine, natural medicine, 2. Development of new varieties of Chinese medicine, as a Chinese medicine necessary for quality control of Chinese medicine Standard product development and application technology. Background technique [0002] Papaya (Fructuschaenomelis) is the dry and nearly mature fruit of Chaenomeles Speciosa (Sweet) Nakai, a plant of the Rosaceae family. It is mainly produced in Xuancheng, Anhui, Qijiang, Chongqing and Ziqiu, Hubei. It is a commonly used traditional Chinese medicine in clinical medicine. The effect of stomach dampness. Its chemical composition has been reported to include organic acids, triterpenoids, flavonoids, saponins, polysaccharides, etc., but there is no in-depth study report on the relationship between chemical composition and its efficacy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 查孝柱
Owner 查孝柱
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