Centella asiatica medicinal material and its extract product and method for detecting mass of preparation

A quality detection method and technology for herbal materials, which are applied in pharmaceutical formulations, material separation, and analysis of materials, etc., can solve problems such as good stability, high detection sensitivity, and inability to objectively and reasonably control the quality of medicinal materials and their preparations, and achieve accurate and sensitive Detection, high sensitivity effect

Active Publication Date: 2018-08-14
GUILIN SANJIN PHARMACEUTICALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has high detection sensitivity and good stability, and can objectively, comprehensively and accurately evaluate the quality of Centella asiatica medicinal materials and their extracts and preparations, and can solve th...

Method used

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  • Centella asiatica medicinal material and its extract product and method for detecting mass of preparation
  • Centella asiatica medicinal material and its extract product and method for detecting mass of preparation
  • Centella asiatica medicinal material and its extract product and method for detecting mass of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] (1) Preparation of asiaticoside reference substance solution

[0077] Accurately weigh 15 mg of asiaticoside, put it in a 10ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well, as a stock solution of asiaticoside reference substance, concentration 1.4464mg / ml; accurately absorb the above-mentioned reference substance stock solution 1.2 ml, put it in a 10ml measuring flask, add methanol to make a reference substance solution of asiaticoside containing 0.1736mg per 1ml.

[0078] (2) Preparation of test solution

[0079] Take about 0.5g of Centella asiatica powder (passed through the No. 2 sieve), accurately weigh it, place it in a stoppered Erlenmeyer flask, accurately add 20ml of 80% methanol, weigh it, and ultrasonically treat it (power 210W, frequency 40kHz) for 30 minutes. Cool, weigh it again, add 80% methanol to make up the weight loss, shake well, filter, take the filtrate, and get it;

[0080] (3) Determination of Asiaticoside

[0081] The asi...

Embodiment 2

[0099] (1) Preparation of asiaticoside reference substance solution

[0100] Accurately weigh 15 mg of asiaticoside, put it in a 10ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well, as a stock solution of asiaticoside reference substance, concentration 1.4464mg / ml; accurately absorb the above-mentioned reference substance stock solution 1.2 ml, put it in a 10ml measuring flask, add methanol to make a reference substance solution of asiaticoside containing 0.1736mg per 1ml.

[0101] (2) Preparation of test solution

[0102] Take about 0.5g of Centella asiatica powder (passed through the No. 2 sieve), accurately weigh it, place it in a stoppered Erlenmeyer flask, accurately add 20ml of 80% methanol, weigh it, and ultrasonically treat it (power 210W, frequency 40kHz) for 30 minutes. Cool, weigh it again, add 80% methanol to make up the weight loss, shake well, filter, take the filtrate, and get it;

[0103] (3) Determination of Asiaticoside

[0104] The asi...

Embodiment 3

[0122] (1) Preparation of asiaticoside reference substance solution

[0123] Accurately weigh 15 mg of asiaticoside, put it in a 10ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well, as a stock solution of asiaticoside reference substance, concentration 1.4464mg / ml; accurately absorb the above-mentioned reference substance stock solution 1.2 ml, put it in a 10ml measuring flask, add methanol to make a reference substance solution of asiaticoside containing 0.1736mg per 1ml.

[0124] (2) Preparation of test solution

[0125] Take about 0.5g of Centella asiatica powder (passed through the No. 2 sieve), accurately weigh it, place it in a stoppered Erlenmeyer flask, accurately add 20ml of 80% methanol, weigh it, and ultrasonically treat it (power 210W, frequency 40kHz) for 30 minutes. Cool, weigh it again, add 80% methanol to make up the weight loss, shake well, filter, take the filtrate, and get it;

[0126] (3) Determination of Asiaticoside

[0127] The asi...

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Abstract

The invention relates to a traditional Chinese medicinal material and a method for detecting mass of a traditional Chinese medicinal material preparation, and concretely relates to a centella asiaticamedicinal material and an extract product and the method for detecting the mass of the preparation. The method employs a high performance liquid chromatography for separating, through a QAMS technology, centella asiatica is taken as an internal standard substance, a relative correction factor of centella asiatica, madecassoside and asiaticoside-B is established, and the content of madecassoside and asiaticoside-B can be calculated. The method has the advantages of high detection sensitivity and good stability, can be objectively, comprehensively and accurately evaluate the mass of the centella asiatica medicinal material and its extract product and its preparation, can solve the problems of non objective and reasonable control of the medicinal material and the preparation mass due to lacking of a contrast product, and has important meaning for controlling mass and guaranteeing curative effect.

Description

Technical field [0001] The invention relates to a quality detection method of traditional Chinese medicinal materials and preparations thereof, in particular to a quality detection method of Centella asiatica herbal materials and extracts and preparations thereof. Background technique [0002] Centella asiatica is a dry whole plant of the Umbelliferae Centella asiatica (L.) Urb. It has various physiological activities such as anti-inflammatory, enhancing memory, promoting collagen synthesis and anti-cancer. It is currently believed that triterpene saponins are Centella asiatica (L.) Urb. The active ingredients of the grass mainly include madecassoside (madecassoside), madecassoside (asiaticoside), and asiaticoside-B (asiaticoside-B) three compounds, the structural formula is shown below, because Centella asiatica Glycoside-B is an isomer of madecassoside. It is difficult to separate and prepare a large number of high-purity reference substances, which directly limits the use of c...

Claims

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Application Information

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IPC IPC(8): G01N30/02A61K36/23
CPCA61K36/23G01N30/02
Inventor 邹节明刘曲山
Owner GUILIN SANJIN PHARMACEUTICALS CO LTD
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