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Method for separating and purifying antibacterial components in cumin essential oil

A technology of cumin essential oil and purification method, which is applied in the preservation of food ingredients as anti-microbial, preservation of meat/fish with chemicals, food ingredients containing natural extracts, etc. problem with good reproducibility

Inactive Publication Date: 2016-11-23
XINXIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the antibacterial effect of cumin essential oil, it is necessary to separate and purify the antibacterial components. However, due to the complex components in cumin essential oil, it is difficult to separate cumin essential oil to obtain high-efficiency antibacterial components using current technical means, which limits its use in the food field. Promoted application of

Method used

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  • Method for separating and purifying antibacterial components in cumin essential oil
  • Method for separating and purifying antibacterial components in cumin essential oil
  • Method for separating and purifying antibacterial components in cumin essential oil

Examples

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Embodiment 1

[0020] 1. First dry and pulverize the commercially available cumin seeds, then weigh the cumin powder, use petroleum ether with a boiling range of 30-60°C as a solvent, and extract it at 60°C by Soxhlet extraction. After the extract was dried with anhydrous sodium sulfate, the solvent was removed with a rotary evaporator to obtain cumin essential oil;

[0021] 2. Adopt column chromatography to separate the saturated hydrocarbons, aromatic hydrocarbons, non-hydrocarbons and colloid four components in the above-mentioned cumin essential oil successively; the elution solvent in the column chromatography is: saturated hydrocarbons use normal hexane, aromatic hydrocarbons n-hexane / dichloromethane=1:2 (volume ratio), dichloromethane / methanol=9:1 (volume ratio) for non-hydrocarbons, methanol for colloids; saturated hydrocarbons, aromatic hydrocarbons, non-hydrocarbons and colloids are required The total yield of each component reaches 85% to 100%, and equal samples are analyzed below...

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Abstract

The invention discloses a method for separating and purifying antibacterial components in cumin essential oil and belongs to the field of separation and extraction of natural products. The method includes the steps that firstly, petroleum ether is used as a solvent to carry out digestion on cumin, and the cumin essential oil is obtained; the cumin essential oil is sequentially separated into saturated hydrocarbon, aromatic hydrocarbon, nonhydrocarbon and colloid through column chromatography; the effective antibacterial components in the essential oil are obtained. Through verification, the extracted cumin essential oil components are remarkable in antibacterial and bacteriostatic effect, and extract from cumin plants can serve as a natural food preservative and is safe and reliable.

Description

technical field [0001] The present invention relates to the separation and purification method of antibacterial components in cumin essential oil, which is a new method for extracting and separating effective antibacterial components in natural plants conveniently and quickly. Cumin essential oil will be widely used as a natural food preservative The application belongs to the field of separation and extraction of natural products. Background technique [0002] Cumin belongs to the herbaceous plant of Umbelliferae, also known as cumin, wild fennel and benzo fennel. Cumin seeds can be used as food condiments, and have a certain inhibitory effect on lipid peroxidation and the ability to protect deoxyribose. In the existing studies on the extraction and chemical composition of cumin volatile oil, methods such as organic solvent extraction, steam distillation and microwave extraction are used to obtain cumin essential oil, and the components of the essential oil are analyzed an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20A23L3/3472A23L3/3517A23L3/3562
CPCA23B4/20A23L3/3472A23L3/3517A23L3/3562A23V2002/00A23V2200/10A23V2250/21
Inventor 周建伟王储备郭晖黄建新褚亮亮
Owner XINXIANG UNIV
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