Method for determining microcystin MC-LR in water body

A technology of MC-LR and microcystin, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high operator risk, many elution steps, large size, etc. Reproducible and fast analysis results

Inactive Publication Date: 2013-04-17
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still the following problems in sample pretreatment: (1) Resin is used as a enrichment column in sample pretreatment, which has a large volume, many elution steps, and consumes a lot of organic solvents, and the organic solvents involve carbon tetrachloride, acetone, etc. Relatively toxic solvents pose a relatively high risk to operators; (2) A conventional C18 silica gel column is used to enrich and purify samples. Because of its large size, more fillers and solvents are required, and there is a waste of solvents and fillers The phenomenon of insufficient utilization, and the low recycling rate of fillers, which are often disposable, have a great impact on the environment

Method used

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  • Method for determining microcystin MC-LR in water body
  • Method for determining microcystin MC-LR in water body
  • Method for determining microcystin MC-LR in water body

Examples

Experimental program
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Embodiment 1

[0044] A method for assaying microcystin MC-LR in water, specifically comprising the steps of:

[0045] (1) Pretreatment of water samples

[0046] Take the tap water sample and add MC-LR standard sample to obtain the tap water sample to be tested, and make the theoretical value of MC-LR in the tap water sample to be 1mg / L.

[0047] Take 5mL of the tap water sample to be tested, filter it through 1.2μm GF / C glass fiber filter paper, collect the filtered tap water sample for use;

[0048] (2) Activation of SPE column and extraction of microcystin MC-LR

[0049] The filler in the SPE column is C18 silica gel, and the SPE filler for each sample column is 500mg. The SPE column is first activated with 5mL of methanol and adjusted with 10mL of Milli-Q water;

[0050] Extract the tap water sample to be tested after filtering in step (1) with the above-mentioned activated SPE column, and enter the filtered tap water sample into the SPE extraction column at a speed of 10mL / min to retain...

Embodiment 2

[0063] A method for assaying microcystin MC-LR in water, specifically comprising the steps of:

[0064] (1) Pretreatment of water samples

[0065] Take 5mL of the artificial lake Xiayang Lake water sample and filter it through GF / C glass fiber filter paper with a specification of 1.2μm, and collect the filtered artificial lake Xiayang Lake water sample for use;

[0066] (2) Activation of SPE (solid phase extraction) column and extraction of microcystin MC-LR

[0067] The filler in the SPE column is C18 silica gel, and the filler in each sample column is 500mg. The SPE column is first activated with 5mL of methanol and adjusted with 10mL of Milli-Q water;

[0068] The filtered water sample of the artificial lake Xiayang Lake to be tested in step (1) is extracted with the above-mentioned activated SPE column, and the filtered water sample of the artificial lake Xiayang Lake to be tested enters the SPE extraction column at a speed of 10mL / min. Retain the isolate, other interfer...

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Abstract

The invention discloses a method for determining microcystin MC-LR in a water body, which belongs to the technical field of environmental protection. The method for determining microcystin MC-LR in the water body utilizes glass fiber (GF)/ C glass fiber filter paper to filter frustules in a water sample to be determined, and a solid-phase extraction column-SPE column which takes C18 silica gel as a filler to separate and purify the filtered water sample to be determined, a high performance liquid chromatography is used to detect, and then, the pre-obtained standard curves of the corresponding peak areas of microcystin MC-LR standard substances with different concentrations is utilized to calculate the content of the microcystin MC-LR in the water sample to be determined. The method for determining microcystin MC-LR in the water body has the advantages of simplicity in steps, convenience in operation, reduction in eluant usage and environmental toxicity, safety in operating personnel, low detection cost and the like, and the minimum detection limit is 0.002mg/L.

Description

technical field [0001] The invention relates to a method for measuring microcystin MC-LR in water, belonging to the technical field of environmental protection. Background technique [0002] With the continuous large-scale outbreak of global freshwater blooms, microcystins, the secondary metabolites released during the death of cyanobacteria, have become a kind of natural toxic substances widely present in water bodies. Microcystin is a heptapeptide monocyclic compound, and so far, 80 different microcystin subtypes have been found. Among them, the microcystin MC-LR, whose variable amino acids are leucine (L) and arginine (R), is the most common and the most toxic. It is currently known to be second only to dioxin in toxicity. Environmental behaviors such as the distribution, migration and accumulation of microcystin MC-LR in the environment have received special attention from environmental protection workers. [0003] At present, the detection methods of microcystin MC-LR...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 叶璟张颖王鲁梅楼春钱景茹
Owner SHANGHAI INST OF TECH
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