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Baobab sterile germination accelerating method and germination accelerating culture medium

A baobab tree and culture medium technology, applied in the direction of culture medium, planting substrate, gardening methods, etc., can solve the problems of difficult seedling breeding, low germination rate, high degree of seed dormancy, etc., to improve the germination rate of seeds and transplant survival rate effect

Inactive Publication Date: 2019-04-02
SANYA NANFAN SCI TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, baobab seeds have a high degree of dormancy, low germination rate, and difficult seedling breeding. Using aseptic germination technology to study the efficient propagation method of its seedlings will lay the foundation for the development and utilization of this edible woody plant, which will contribute to the development of tropical woody resources. Conservation and innovative utilization are of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for aseptic germination acceleration of baobab, comprising the following steps:

[0022] Baobab seeds were acid-etched with 80% concentrated sulfuric acid for 6 hours, washed away the sulfuric acid, washed 5 times with detergent, shaken for 10 minutes each time, rinsed with running water for 40 minutes, then soaked in 80% alcohol and shaken for 1 minute , after rinsing with sterile water for 3 times, add mass concentration 0.3% HgCl 2 After soaking and stirring the solution for 15 minutes, discard the waste liquid and wash it with sterile water for 5 times; then inoculate the seeds into the germination medium (WPM medium) and place them at a culture temperature of 24°C±2°C for 24 hours in shade, and then transfer them to light The intensity is 2000~2500lx, and the cultivation temperature is 24°C±2°C in a culture room, and the light is 8 hours a day.

Embodiment 2

[0024] A method for aseptic germination acceleration of baobab, comprising the following steps:

[0025] Baobab seeds were etched with 95% concentrated sulfuric acid for 12 hours, washed away the sulfuric acid, washed 3 times with detergent, shaken for 5 minutes each time, rinsed with running water for 30 minutes, then soaked in 75% alcohol and shaken for 3 minutes , after washing with sterile water for 3 times, add mass concentration of 0.1% HgCl 2 After soaking and stirring the solution for 15 minutes, discard the waste liquid and wash it with sterile water for 5 times; then inoculate the seeds into the germination medium (WPM medium) and place them at a culture temperature of 24°C±2°C for 48 hours in shade, and then transfer them to light The intensity is 2000~2500lx, the cultivation temperature is 24°C±2°C in a culture room, and the light is 10h every day.

Embodiment 3

[0027] The difference between embodiment 3 and embodiment 2 is that

[0028] The germination medium is based on the WPM medium, and the germination medium also includes the following components: rue herb extract 0.5g / L, lemongrass extract 7g / L, garlic extract 0.7mg / L, sugar beet Alkali 10mg / L, indole butyric acid 3mg / L.

[0029] The preparation method of the rue herb extract is as follows: crush the rue herb, pass through a 300-mesh sieve, soak in absolute ethanol for 30min, the mass ratio of rue rue and absolute ethanol is 1:2, then recover ethanol, dry, and obtain Rue Herb Extract.

[0030] The lemongrass extract is lemongrass essential oil.

[0031] The preparation method of the garlic extract is as follows: peel and wash 10 parts by weight of garlic, add 20 parts by weight of water, soak at 10°C for 30 minutes, pass through a 50-mesh sieve to obtain a filtrate, and concentrate the filtrate under reduced pressure to reduce the volume to the original volume 10% of the con...

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Abstract

The invention discloses a baobab sterile germination accelerating method and a germination accelerating culture medium. The method includes: using concentrates sulfuric acid for acid etching, using aliquid detergent for flushing, using flowing water for flushing, using alcohol for soaking, using sterile water for flushing, using a HgCi2 solution for soaking, using sterile water for cleaning, andculturing a germination accelerating culture medium, wherein the germination accelerating culture medium contains cymbopogon distans extract, lemon grass extract, garlic extract, betaine and indolebutyric acid. The defect of high-degree dormancy of baobab seeds is overcome; by the method, baobab seed germination rate is up to higher than 90%, transplanting survival rate is up to higher than 88%, baobab seedlings are uniform in growing vigor, and the method is high in repeatability. The seed germination rate and the transplanting survival rate of baobab are increased greatly, a lot of baobab seedlings with uniform growing vigor can be provided within a short time, and the method is of great significance in baobab production.

Description

technical field [0001] The invention relates to a method for accelerating germination of baobab and a medium for accelerating germination, belonging to the technical field of plant seed germination. Background technique [0002] Baobab is a commonly used vegetable in Africa. Its tubers, twigs, fruits, seeds, leaves and flowers are all edible, with good taste and unique flavor; its fruits, bark and leaves can be used as medicine and have a certain ornamental value because of their strange shape. It is a tropical woody plant worthy of development and utilization. The woody vegetable baobab has the advantages of resistance to typhoon and rain, barrenness, high temperature, and waterlogging. It is slightly harmed by harmful organisms such as diseases, insects, and grasses. It has many branches, tender leaves and high nutritional value, which is conducive to the development of special dishes and is suitable for Hainan's special tourism market. However, baobab seeds have a high ...

Claims

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Application Information

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IPC IPC(8): A01C1/02A01G17/00A01G7/06A01G24/20A01G24/22A01G24/30
CPCA01C1/02A01G7/06A01G17/005A01G24/20A01G24/22A01G24/30
Inventor 刘扬陈冠铭李宏杨钟祥涛
Owner SANYA NANFAN SCI TECH INST
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