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Detection method of nine-component heart-calming particle

A detection method and particle technology, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, can solve the problems of ineffective determination of ginsenosides in Jiuwei Zhenxin Granules, mutual interference, and inability to separate columns, etc. Achieve good separation effect, good baseline separation effect and good precision effect

Active Publication Date: 2013-10-09
BEIJING BEILU PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the complex composition, it is easy to interfere with each other in the identification experiment, and it is difficult to accurately identify
At the same time, due to the discovery of ginsenoside Rg in Jiuwei Zhenxin Granules in the liquid phase pre-experiment for content determination 1 The retention time is relatively close to that of ginsenoside Re, and most brands of columns cannot separate them, and there are 2-3 impurities around the two-component chromatographic peaks. Therefore, it is difficult to successfully separate the above two chromatographic peaks, let alone the effective determination of Jiuwei Zhenxin Ginsenoside Rg in granule 1 and the content of ginsenoside Re

Method used

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  • Detection method of nine-component heart-calming particle
  • Detection method of nine-component heart-calming particle
  • Detection method of nine-component heart-calming particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Identification of Suanzaoren in Jiuwei Zhenxin Granules

[0053] Take a sample of Jiuwei Zhenxin Granules, grind it finely, weigh 20g, add 100ml of methanol, seal it, weigh it, soak overnight, heat and reflux for 2 hours, let it cool, use methanol to make up the lost weight, filter, and accurately measure Take 50ml of the filtrate, evaporate to dryness, add 30ml of water to the residue to dissolve, shake and extract with 30ml of diethyl ether, discard the diethyl ether, shake and extract 3 times with n-butanol saturated with water, 30ml each time, combine the n-butanol solution, and wash with ammonia The test solution (purchased from Beijing Chemical Factory, model: 20110102) was washed twice, 35ml each time, combined with the ammonia test solution, extracted by shaking with 35ml of n-butanol saturated with water, combined with the n-butanol solution, and washed with n-butanol-saturated water Wash with 30ml, discard the water, separate the n-butanol solution,...

Embodiment 2

[0054] Example 2: Identification of Schisandra in Jiuwei Zhenxin Granules

[0055] Weigh 5 g of the sample of Jiuwei Zhenxin granule, grind it finely, add 40 ml of chloroform, place it on a water bath and heat it under reflux for 3 hours, filter, evaporate the filtrate to dryness, add 1 ml of chloroform to the residue to dissolve, and use it as a sample for testing. product solution. Take another 1 g of Schisandra reference medicinal material, and prepare the reference medicinal material solution in the same way as the test solution 2 prepared in the identification method of Schisandra chinensis. Then take Schizandrin B reference substance, add chloroform to make a solution containing 1mg per 1ml, as the reference substance solution. According to the thin-layer chromatography test, draw the test solution, the reference drug solution and the reference solution respectively 4μl, 4μl, 4μl, and respectively point on the same silica gel GF 254 On the thin-layer board, the upper l...

Embodiment 3

[0056] Example 3: Identification of Corydalis Corydalis in Jiuwei Zhenxin Granules

[0057] Weigh 5g of the Jiuwei Zhenxin granule, grind it finely, add 1ml of concentrated ammonia test solution, 20ml of chloroform, ultrasonic treatment for 40 minutes, filter, evaporate the filtrate to dryness, add 1ml of ethanol to the residue to dissolve, and use it as the test solution . In addition, 1 g of Corydalis Corydalis reference medicinal material was taken, and the reference medicinal material solution was prepared in the same way as the test solution 3 prepared in the identification method of Corydalis Corydalis. Then take the tetrahydropalmatine reference substance, add ethanol to make a solution containing 1mg per 1ml, as the reference substance solution. According to the thin-layer chromatography test, draw the test solution, the reference medicinal material solution and the reference solution in 20 μl, 5 μl, and 2 μl respectively, and place them on the same silica gel G thin-...

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Abstract

The invention discloses a detection method of a nine-component heart-calming particle. The detection method of the nine-component heart-calming particle can carry out thin-layer chromatography on spina date seed, schisandra chinensis, corydalis tuber, cinnamon, asparagus fern and polygala tenuifolia in the nine-component heart-calming particle, microscopic identification on calcium oxalate in ginseng and determine HPLC (high performance liquid chromatography) content of ginsenoside Rg1 and ginsenoside Re. The detection method of the nine-component heart-calming particle has the characteristics of stability, high precision, good reproducibility, high recovery ratio and good separation effect. By adopting the detection method of the nine-component heart-calming particle, the quality of the nine-component heart-calming particle can be further comprehensively controlled, the provided method for determining the content of ginsenoside Rg1 and ginsenoside Re can realize good baseline separation effect on ginsenoside Rg1 and ginsenoside Re, and a determination result is accurate and the reproducibility is good.

Description

technical field [0001] The present invention relates to the detection method of Jiuwei Zhenxin Granules, in particular, to the identification method of various components in Jiuwei Zhenxin Granules and ginsenoside Rg 1 And the assay method of ginsenoside Re content. Background technique [0002] Jiuwei Zhenxin Granules are mainly used to nourish the heart and nourish the spleen, replenish qi and calm the nerves, and are used to treat generalized anxiety syndrome with deficiency of both the heart and spleen, such as clinical symptoms such as pensive thoughts, insomnia, dreaminess, palpitations, loss of appetite, and mental fatigue Limbs, dizziness, sweating easily, Taixi, sallow complexion, pale tongue with thin white coating, deep thready pulse. Jiuweizhenxin granules are slightly fragrant, bitter, and fine brown granules. The main ingredients of Jiuwei Zhenxin Granules include Suanzaoren, Schisandra, Corydalis Corydalis, Cinnamon, Asparagus, Polygala, and Ginseng. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N21/00G01N30/02G01N30/36
Inventor 傅欣彤范妙璇杨俊东
Owner BEIJING BEILU PHARM CO LTD
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