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Method for determining content of free nicotine in tobaccos and tobacco products

A technology of tobacco products and determination methods, which is applied in the direction of measuring devices, material separation, instruments, etc., can solve the problems of environmental damage of free nicotine, and achieve the effects of accurate and reliable measurement results, accurate measurement results, and less use of solvents

Active Publication Date: 2011-11-02
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The above-mentioned methods all have different degrees of damage to the environment in which free nicotine exists.

Method used

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  • Method for determining content of free nicotine in tobaccos and tobacco products
  • Method for determining content of free nicotine in tobaccos and tobacco products
  • Method for determining content of free nicotine in tobaccos and tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The main reagents and instruments used

[0040] Nicotine: purity ≥ 99%;

[0041] n-Hexane: chromatographically pure, Fisher Company, USA;

[0042] 3R4F reference cigarette, University of Kentucky, USA;

[0043] Gas chromatography / mass spectrometry with headspace solid-phase microextraction autosampler, model 6890 / 5973, Agilent, USA;

[0044] ZB-624 elastic capillary column with 14% cyanopropylbenzene and 86% dimethyl polysiloxane, with a length of 30 m, an inner diameter of 0.32 mm, and a film thickness of 1.8 μm, Phenomenex, USA;

[0045] 65 μm polydimethylsiloxane / styrene solid-phase microextraction head, partially cross-linked, Supelco, USA;

[0046] Analytical balance, with a sensitivity of 0.1 mg;

[0047] Manual micro-sampling needle;

[0048] Brown volumetric flasks of various specifications;

[0049] 1. Establishment of standard working curve

[0050] 1.1 Preparation of blank samples

[0051] Grind 50 g of shredded tobacco into powder, add 1 L of 2% NaOH a...

Embodiment 2

[0092] Carry out according to embodiment 1, and make corresponding adjustments in the following aspects:

[0093] The 2002 Chongqing Wanzhou Burley tobacco sheet and the 2009 Qujinghong large sheet tobacco were respectively selected, and the above two samples were shredded to obtain shredded tobacco samples with a cutting width of 0.9 mm. Tobacco dust samples were obtained by grinding the shredded tobacco through a cyclone mill. The above six samples of tobacco sheet, shredded tobacco, and tobacco powder were respectively tested for free nicotine in the samples, and the results are shown in Table 5. The results show that the determination result of the tobacco powder is higher than that of other forms of tobacco products, so the method adopts the tobacco powder as the experimental object.

[0094]

Embodiment 3～5

[0096] Carry out according to embodiment 1, and make corresponding adjustments in the following aspects:

[0097] In order to investigate the impact of different sample weights on the test results, Example 3 adopted a 0.1 g sample weight, Example 4 adopted a 0.2 g sample weight, and Example 5 adopted a 0.5 g sample weight. Under different weighing conditions, 5 samples were processed in parallel, and the coefficient of variation of the test results of the samples was investigated. The test results showed that: when the weighing amount was 0.2 g, free nicotine and deuterated toluene met the analysis and test requirements, and the results are shown in Table 6. .

[0098]

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Abstract

The invention discloses a method for determining the content of free nicotine in tobaccos and tobacco products, and the method provided by the invention comprises the following steps: carrying out solid-phase micro extraction on a tobacco powdery sample by using dimethyl polysiloxane / styrene as an extraction material; and carrying out quantitative analysis by using a gas chromatography mass spectrometer, thus obtaining the free nicotine determination result. Method evaluation data shows that the method has the advantages of accurate determination result, good reproducibility, less destructionof the balance between free nicotine and protonic nicotine and the like.

Description

technical field [0001] The invention belongs to the technical field of cigarette evaluation, in particular to a method for measuring free nicotine in tobacco and tobacco products. Background technique [0002] Nicotine is a unique organic alkaloid in tobacco that contains pyridine and pyrrole rings, and plays an important role in tobacco products, and is one of the main targets of cigarette consumption. Since the last century, the content and physiological effects of nicotine, especially free nicotine, in cigarette smoke have stimulated more research interests of scientists. In 1970, Armitage (Armitage, Nature, 1970) found that free nicotine has stronger lipophilicity than proton nicotine, and it is easy to penetrate the oral mucosa and be absorbed by the body more quickly. People focus on the absorption and metabolism of free nicotine in the human body (Schievelbein, Agents Actions 1973; Taylor, Proc. Natl. Acad. Sci. 2010), the addiction of free nicotine, and the relation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/14
Inventor 廖头根者为王明锋李先毅段焰青何靓王坚
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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