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Detection method for contents of varieties of flavonoids in taxus chinensis branches and preparation method of contents of varieties of flavonoids in taxus chinensis branches.

A detection method, the technology of yew, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of good repeatability, time saving and good reproducibility

Active Publication Date: 2018-10-09
HUBEI XIANGRUIFENG ECOLOGICAL FRUIT IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on the content of flavonoids in Taxus chinensis, and only a small number of literatures report the content of taxus flavonoids monomers. Comparative document 2: "HPLC determination of two kinds of biflavonoids in the leaves of Taxus chinensis, Ni Liang, Inner Mongolia Traditional Chinese Medicine , 2014, 33(34):126-127 "determined the content of biflavonoids and biflavones in yew; comparative document 3: "Determination methods and seasonal changes of taxane compounds, flavonoids and polysaccharides in the needles of Taxus chinensis, Chen Fang, Xinjiang Agricultural University, 2016.5 "Determination of the contents of quercetin, spicantha biflavone and golden pine biflavone in yew

Method used

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  • Detection method for contents of varieties of flavonoids in taxus chinensis branches and preparation method of contents of varieties of flavonoids in taxus chinensis branches.
  • Detection method for contents of varieties of flavonoids in taxus chinensis branches and preparation method of contents of varieties of flavonoids in taxus chinensis branches.
  • Detection method for contents of varieties of flavonoids in taxus chinensis branches and preparation method of contents of varieties of flavonoids in taxus chinensis branches.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 A kind of detection method of the content of various flavonoids in the branch of Chinese yew

[0020] 1. Instrument and reagent information

[0021] Waters e2695 high performance liquid chromatograph (Waters, USA), one-ten-thousandth balance (Changzhou Tianzhiping Instrument Equipment Co., Ltd.), miiner ultrapure water machine, electric blast drying oven (Wuhan Jinbaohua Technology Co., Ltd.) , Ultrasonic cleaning machine (Shenzhen Jingzuancheng Ultrasonic Equipment Co., Ltd.), 5-5N low-speed centrifuge (Hunan Hengnuo Instrument Equipment Co., Ltd.), GZY scientific research and pulverizer (Shanghai Gaozhi Precision Instrument Co., Ltd.).

[0022] Rutin, quercetin, amiflavones of Suicha, isoflavones of ginkgo biloba, and biflavones of Jinpine (Shanghai Yuanye Biotechnology Co., Ltd.), chromatographic grade methanol, analytical grade methanol, analytical grade ethanol, analytical grade petroleum ether, analysis grade sodium hydroxide, and water was ultrapure...

experiment example 1

[0037] The selection of experimental example 1 liquid chromatography condition

[0038] 1. In the selection of the mobile phase, the two mobile phase systems of methanol-water and acetonitrile-water were compared, and it was found that when the acetonitrile-water system was used as the mobile phase, the tailing of the main peaks of isoflavones of isoginkgo biloba and biflavones of golden pines was more severe, so the choice of Methanol-water system was used as the mobile phase; the five flavonoid compounds had large polarity differences and could not be separated by isocratic elution, so gradient elution was used. After adjustment, the main peak of each compound was completely separated from the impurity peak.

[0039] 2. Selection of measurement wavelength

[0040] Take each reference substance solution with a concentration of 40 μg / mL and scan it with a full-wavelength ultraviolet spectrophotometer, and find that the maximum absorption wavelengths of the five flavonoids are ...

experiment example 2

[0041] The selection of experimental example 2 test article processing method

[0042] 1. Selection of extraction solvent for the test product

[0043] Precisely weigh four portions of 1.00g of yew branch powder, put them in a centrifuge tube, add 30mL of petroleum ether, ultrasonically degrease twice, 30min each time, dry the degreased residue at 60°C, add ethanol and ethanol to the dried residue respectively -water (80:20), pH 10 ethanol-water (80:20), methanol 30mL ultrasonic extraction, extraction 3 times, 30min each time, the combined extracts were dried by rotary evaporation, the residue was dissolved with an appropriate amount of methanol solution, and Dilute to 10mL. The solution was obtained by filtering with a 0.45 μm filter membrane, and the content results of each flavonoid were determined. The results are shown in Table 2.

[0044] Table 2 Comparison of extraction effects with different extraction solvents

[0045]

[0046] It can be seen from Table 2 that t...

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Abstract

The invention discloses a detection method for contents of varieties of flavonoids in taxus chinensis branches. The detection method is used for detecting contents of rutin, quercetin, amentoflavone,isoginkgetin and sciadopitysin and comprises the following steps: preparing a test sample solution; preparing a control product solution; performing high performance liquid chromatography measurementand respectively injecting the test sample solution and the control product solution into a high performance liquid chromatograph to be detected, wherein the chromatographic conditions are as following: C18 is utilized as a chromatographic column, a methyl alcohol-water mixed solution is utilized as a flow phase, gradient elution is performed, detection wavelength is 330nm, a flow velocity is 1.0mL / min, a column temperature is 30 DEG C, and the number of theoretical plates is not lower than 3000. The detection method disclosed by the invention utilizes the high performance liquid chromatography to detect contents of the rutin, the quercetin, the amentoflavone, the isoginkgetin and the sciadopitysin in the taxus chinensis branches at the same time; the method has the advantages of simpleness, good repeatability, high recovery rate, ability in accurately measuring the contents of 5 kinds of flavonoids in the taxus chinensis branches, convenience and quickness.

Description

technical field [0001] The invention relates to the technical field of extraction and detection of flavonoids, in particular to a method for detecting the contents of various flavonoids in yew branches. Background technique [0002] Taxus is a plant of the genus Taxus in the family Taxaceae, also known as Taxus. It is recorded in "Chinese Materia Medica" and "Dictionary of Traditional Chinese Medicine" that "Chinese yew "eliminates water and reduces swelling, mainly treats nephritis, edema, and difficulty in urinating"; "Records the meridian distribution of nature, flavor and function of the southern yew: "It is slightly sweet, bitter, and flat, and returns to the kidney and heart meridian." Accumulation, edema, dysuria, rheumatic arthralgia, etc. [0003] Bioflavonoids have excellent pharmacological activities, such as improving the body's immunity, anti-inflammation, protecting the liver, sterilizing, anti-virus, and anti-rheumatism. To develop a detection method for the...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 李晓胡亮
Owner HUBEI XIANGRUIFENG ECOLOGICAL FRUIT IND CO LTD
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