42 results about "Family Cucurbitaceae" patented technology

The invention discloses a method for eliminating continuous cropping obstacles. The method comprises the steps of: grafting cucurbitaceous pumpkin, opo squash, calabash or wax gourd on a rootstock of the cucurbitaceous pumpkin, opo squash, calabash or wax gourd; after a sprout which is subject to a successful grafting grows a section of stem, grafting a watermelon or cucumber seedling coin on the growth stem section so as to realize the effect of avoiding continuous cropping obstacles of watermelon or cucumber, promoting the production of watermelon and cucumber and improving the land utilization rate and economic benefits of farmers. By adopting a secondary grafting method and respectively using crops of the same family but different genera or different categories as the rootstock and the coin for grafting, the invention avoids continuous cropping obstacles generated after long-term continuous cropping.

The present disclosure relates to methods of producing a sweet juice composition from monk fruit and other fruit of the Cucurbitaceae family containing mogroside V and other terpene glycosides. The methods employ a cation exchange resin and an anion exchange resin to obtain a sweet juice composition with a clean flavor. The sweet juice composition can be used in a food, beverage, pharmaceutical or dietary supplement product.

A method of preparing a natural sweetener from a heat-processed powdered fruit extract of the cucurbitaceae family, such as luo han guo is provided. In one embodiment, the method provides a cleaner tasting and concentrated natural sweetener in which objectionable flavors, odors, colors, and insoluble components are removed to form the natural sweetener.

The invention discloses a preparing method and an application of a sample for detecting cucumber green mottle mosaic viruses (CGMMV) carried by cucurbitaceae seeds. The preparing method includes the steps that starting from treating seed samples, shell-kernel separating treatment is carried out on the cucurbitaceae seeds, seed kernels with the low virus content are removed, seed shells with the high virus content are left to serve as to-be-tested samples, the seed samples obtained after treatment are sufficiently grinded through a full-automatic rapid sample grinding instrument, and the CGMMV in the large quantity of cucurbitaceae seeds can be rapidly detected through the sufficiently-grinded samples with a RT-PCR method and can also be rapidly detected through the sufficiently-grinded samples with a dot enzyme-linked immunosorbent assay (dot-ELISA) method. The preparing method and the application are suitable for virus detection of the common cucurbitaceae seeds such as calabash seeds, cucumber seeds, lagenaria sicerariae seeds, pumpkin seeds, long gourd seeds and watermelon seeds. A detecting method is high in sensitivity, good in specificity, short in consumed time and low in cost, technical supports are provided for large-scale detection and scientific prevention and control of the CGMMV carried by the cucurbitaceae seeds, and references are provided for detection of plant viruses transmitted by other seeds.

The present invention relates to the treatment of plant material, especially seeds. The method uses vacuum pressure for disinfecting seeds from pathogens. Seed disinfection is important as seeds infected by pathogens could propagate them and lead to high losses of the crops. The method is applicable to seeds from cucurbit species specifically watermelon, melon, squash, pumpkin, cucumber and gourds but also seeds from solanaceous species specifically tomatoes, peppers and eggplants.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention relates to a nematicidal composition containing fluensulfone and iprodione. The effective components ar the fluensulfone and the iprodione with the mass ratio being (1-25):(25-1) in a binary combination manner; in a preparation, the mass-part ratio of the fluensulfone and the iprodione is 1%-80%, and the balance is auxiliary components allowable and acceptable to use in pesticides. The nematicidal composition comprises the following preparation forms such as missible oil, a suspending agent, a wettable powder agent, a water agent, a water dispersible granule agent, a water emulsion agent, a microemulsion agent, a granule agent and a microcapsule agent and is mainly used for preventing and treating insect pests of nematode and the like of solanaceae vegetables, cucurbitaceous vegetables, lettuces and other leafy vegetables.

The invention relates to the technical field of microorganism application, in particular to a bacillus velezensis BV03 inoculant, a preparation method thereof and application thereof in growth of solanaceae plants and / or cucurbitaceae plants. The bacillus velezensis BV03 is already preserved in China General Microbiological Culture Collection Center, the preservation address is No.3, Yard 1, Beichen west road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18936, and the preservation date is November 11, 2019, and the bacillus velezensis is classified and named as bacillus velezensis. The preparation method comprises the following steps: acquiring a seed liquid, fermenting the seed liquid to obtain a fermentation liquid pre-preparation, and preparing the inoculant. The growth promoting effect of the inoculant in industrial seedling culture reaches 30%, the seedling strengthening index is increased by more than 45%, the yield increasing effects are 48% and 34% respectively when the inoculant is used in transplanting, and the fruiting rate is more than 85%.

The present invention relates to a Cucurbita plant, in particular a squash plant, having wide spectrum resistance to potyvirus such as Zucchini Yellow Mosaic Virus (ZYMV), Watermelon Mosaic Virus (WMV), Papaya Ringspot Virus (PRSV) and Moroccan watermelon mosaic virus (MWMV). Methods of selecting a squash plant having wide spectrum potyvirus resistance by marker assisted breeding are also provided.

The invention discloses a method for pierce-inoculating geminivirus infectious clone-containing solid colony to plant, and an application of the method. The method comprises the steps of coating geminivirus infectious clone-containing Agrobacterium tumefaciens EHA105 on YEP solid culture medium containing 100 mg / L kanamycin and 50 mg / L rifampicin; culturing into colony at 28 DEG C; picking colony with toothpicks; piercing 3-4 points on phloem of plant after three-leaf stage, at position 1-2 cm from root; culturing inoculated plant in a greenhouse; and observing virus symptom. The inoculation method provided by the invention has the advantages of no need for raising Bemisia tabaci, high inoculation efficiency and repeatability, simple and rapid operation, and suitability for large-scale popularization. The method provided by the invention is applicable to 16 kinds of geminivirus with severe breakout in our country. The inoculated plants are in families of Solanaceae, Cucurbitaceae and Convolvulaceae. The method can be used for pathogenicity identification of geminivirus and virus gene function research, anti-geminivirus plant variety determination and its breeding.

The invention discloses a tobacco-shred-similar object produced with cucurbitaceae vegetables and an electric heating method of the tobacco-shred-similar object produced with the cucurbitaceae vegetables. The tobacco-shred-similar object produced with the cucurbitaceae vegetables comprises tobacco flavors, nicotine solutions and the shredded cucurbitaceae vegetables, the raw materials which comprise, by weight, 0.03-0.06 parts of the tobacco flavors, 3-8 parts of the nicotine solutions and 55-120 parts of the shredded cucurbitaceae vegetables are evenly mixed, the nicotine solutions are nicotine aqueous solutions or nicotine ethanol solutions, and the nicotine release amount is 0.4-1.2 parts by weight. By means of the tobacco-shred-similar object and the electric heating method, the tobacco-shred-similar object is produced with the cucurbitaceae vegetables, the tobacco flavors and the nicotine solutions, and the health care effect and the medical effect of the cucurbitaceae vegetables are sufficiently used by the tobacco-shred-similar object; compared with a traditional cigarette, the smoking experience of the tobacco-shred-similar object is comfortable and abundant. In the smoking mode, the electric heating method is adopted, smoke can be generated by electrically heating the tobacco-shred-similar object, a smoker can conveniently have a smoke, pollution to the environment is reduced, and harms of hazardous substance burning of the traditional cigarette on the human body are reduced.

The present invention is related to an alcohol compound isolated from the extract of Cucurbitaceae family plant having anti-adipogenic and anti-obesity activity, and a composition comprising the same. The compound showed potent reducing activity of body weight, decreasing effect on the blood triglyceride and cholesterol level, activating activity of PPAR α and preventing activity from the adipogenesis of precursor fat cells with no toxicity, therefore, those extract can be useful in treating or preventing obesity and adipogenesis-involved diseases as a medicine or health care food.

The invention discloses a botanical insecticide and a preparation method thereof. Active components of the botanical insecticide comprise a petroleum ether extract of cucumis melo all-grass, wherein the cucumis melo all-grass is a leaf of Cucumis melo L. which is a cucurbitaceae cucumis plant. According to the invention, effective parts are extracted from the cucumis melo all-grass to be used as active components of the insecticide, so that the petroleum ether extract of cucumis melo all-grass, which can be used for effectively killing aphids, is provided, and a new thought is provided for pest control in agricultural production.

The invention discloses a mogroside synthesis regulation gene Sgesph7 and application thereof, and belongs to the field of biotechnology. According to the mogroside synthesis regulation gene Sgesph7 and the application thereof, by means of whole genome sequencing of Siraitia grosvenorii(Swingle)C.Jeffrey and the analysis of the RNA-seq sequence of different tissue, secondary metabolic pathways associated with the tissue specificity and characters are utilized, and therefore the Siraitia grosvenorii(Swingle)C.Jeffrey epoxide hydrolases Sgeph7 gene with Siraitia grosvenorii(Swingle)C.Jeffrey fruits specifically expressed; and the content of the mogroside in the Siraitia grosvenorii(Swingle)C.Jeffrey fruits can be regulated and controlled by adjusting the expression of the gene, and the geneis used for obtaining a new variety or new strain of the Siraitia grosvenorii(Swingle)C.Jeffrey with the high mogroside content and has important theoretical and practical significance in research ofthe taste mechanism of fruits of cucurbitaceae plants and the breeding work of the taste of the fruits of the cucurbitaceae plants.

Food protecting agent composition having a pH of between 5.5 and 7.5 containing at least 2000 mg / l, preferably at least 1800 mg / l of a combination food protecting agent and guanidinium derivatives, particularly to combinations of oligo(2-(2-ethoxy)ethoxy ethyl guanidinium chloride), poly(hexamethylendiamine guanidinium chloride), polyetheramines, triethyleneglycol diamine, enzymes, PGPR, amino acids, antioxidants such as humic acids and some natural products like phytotherapeutic plant extracts.The purpose of any pre-harvest plant protection program for fruit, cutflowers and vegetables (fruit and vegetables are selected from cereals, e.g. wheat, barley, rye, oats, rice, sorghum and the like; beets, e.g. sugar beet and fodder beet; pome and stone fruit and berries, e.g. apples, pears, plums, peaches, almonds, cherries, strawberries, raspberries and blackber leguminous plants, e.g. beans, lentils, peas, soy beans; oleaginous plants, e.g. rape, mustard, poppy, olive, sunflower, coconut, castor-oil plant, cocoa, ground-nuts; cucurbitaceae, e.g. pumpkins, gherkins, melons, cucumbers, squashes; fibrous plants, e.g. cotton, flax, hemp, jute; citrus fruit, e.g. orange, lemon, grapefruit, mandarin; tropical fruit, e.g. papaya, passion fruit, mango, carambola, pineapple, banana; vegetables, e.g. spinach, lettuce, asparagus, brassicaceae such as cabbages and turnips, carrots, onions, tomatoes, potatoes, hot and sweet peppers; laurel-like plants, e.g. avocado, cinnamon, camphor tree; or plants such as maize, tobacco, nuts, coffee, sugar-cane, tea, grapevines, hops, rubber plants, as well as ornamental plants, e.g. cutflowers, roses, gerbera and flower bulbs, shrubs, deciduous trees and evergreen trees such as conifers) is to prevent the development of diseases that might impair the final quality of the fruit, cutflowers and vegetables and to obtain adequate production yields. The system for post-harvest application of plant protection products on fruit, cutflowers and vegetables during packing aims to safeguard the health of the fruit and vegetables during the period of storage and transport to the final consumer. Since, moreover, the environmental and economic requirements imposed on modern-day fungicides are continually increasing, with regard, for example, to the spectrum of activity, toxicity, selectivity, application rate, formation of residues, and favorable preparation ability, and since, furthermore, there may be problems, for example, with resistances developing to known active compounds, a constant task is to develop new fungicide agents which in some areas at least have advantages over their known counterparts. Therefore, there is still a need to find and / or develop other bacterial, viral and fungicides for storage disease control. Some of the chemical as such are already known. It is also known, that these compounds can be used as more healthy and applicable material.

The invention relates to the field of treatment of agricultural wastes, in particular to the field of treatment of rotten vegetable leaves, and more particularly relates to a resourceful treatment method of stalk and vine rotten vegetable leaves. The stalk and vine rotten vegetable leaves can be treated to form raw materials capable of being used for a seedling culture substrate through microwave disinfection and cow dung co-fermentation, and the raw materials are further matched with other auxiliary materials for resourceful treatment to form the seedling culture substrate. Tests show that the seedling culture substrate formed by mixing the treated rotten vegetable leaves with vermiculite, turf and perlite, can meet the seedling culture nutritional requirements of cucumbers (cucurbitaceae), tomatoes (solanaceae) and cabbages (cruciferae), and is an excellent universal seedling culture substrate.

The invention discloses a cucurbitaceae seed shell processing method, belongs to the technical field of agriculture and relates to a shell processing method. The processing method includes the following steps: (1) the step of seed soaking, namely the step of soaking seeds in warm water with the temperature of 50-55 DEG C for 3-5 h, and soaking the seeds in cold water with the temperature of 20 DEGC for 5-7 h; (2) the step of caruncle removing, namely the step of selecting the middle horizontal line in two sunken parts formed by the two sides of caruncle and the ridge lines of the soaked seeds, and cutting off the upper parts of the caruncle from the middle of the two sunken parts along the horizontal line; (3) the step of accelerating germination, namely the step of performing accelerating germination on the seeds whose caruncle is removed at 25-28 DEG C, and performing sowing after the lengths of root buds reach 3-5 mm; (4) the step of seedbed disinfection, namely the step of performing spraying on the seedbeds per square meter with a disinfectant before sowing, and performing disinfection; and (5) the step of routine management, namely the step of performing sowing on the seeds,and performing covering management after sowing. The method can shorten emergence time, enhance emergence rates, eliminate rotten seeds and buds and reduce costs.

A thrips control agent in accordance with an embodiment of the present invention is used for plants belonging to family Brassicaceae, family Solanaceae, family Rosaceae, family Cucurbitaceae, family Fabaceae, or family Asteraceae. The thrips control agent contains prohydrojasmon as an active ingredient, so that a negative impact of the thrips control agent on plant growth is suppressed.

Use of a composition with fungicidal phytosanitary activity, which has as an active ingredient cinnamic aldehyde combined with terpene alcohol, for treatment against the disease known as powdery mildew in horticultural crops, especially of the Cucurbitaceae family, and more specifically, melon, watermelon, cucumber and courgette, as well as in pepper, strawberry and raspberry crops. The invention presented provides the main advantages of a high efficacy against powdery mildew, great stability over time, and the very low toxicity of the composition.

The invention discloses a method for pierce-inoculating geminivirus infectious clone-containing solid colony to plant, and an application of the method. The method comprises the steps of coating geminivirus infectious clone-containing Agrobacterium tumefaciens EHA105 on YEP solid culture medium containing 100 mg / L kanamycin and 50 mg / L rifampicin; culturing into colony at 28 DEG C; picking colony with toothpicks; piercing 3-4 points on phloem of plant after three-leaf stage, at position 1-2 cm from root; culturing inoculated plant in a greenhouse; and observing virus symptom. The inoculation method provided by the invention has the advantages of no need for raising Bemisia tabaci, high inoculation efficiency and repeatability, simple and rapid operation, and suitability for large-scale popularization. The method provided by the invention is applicable to 16 kinds of geminivirus with severe breakout in our country. The inoculated plants are in families of Solanaceae, Cucurbitaceae and Convolvulaceae. The method can be used for pathogenicity identification of geminivirus and virus gene function research, anti-geminivirus plant variety determination and its breeding.

The invention provides a molecular recognition method for transmission of GAPC1 gene mRNA between cucurbitaceae rootstocks and scions. The molecular recognition method comprises the following steps: 1) grafting by taking cucumbers as scions and taking pumpkins as rootstocks; 2) designing specific primers of the GAPC1 genes of the cucumbers and the pumpkins; 3) performing RT-PCR on cucumbers, pumpkins and grafted cucumbers and pumpkins by using the primers, and comparing RT-PCR product conditions before and after grafting, and 4) constructing a cloning vector of GAPC1. The method provided by the invention is quick, sensitive, high in accuracy, simple and convenient, and can be applied to other cucurbitaceae plants.

The present invention is in the field of agricultural pathogen control by inducing systemic plant defense. More specifically, the present invention relates to an extract of a plant belonging to the family Cucurbitaceae and its use for protecting plants from plant pathogens, in particular parasitic nematodes. The invention also provides a composition containing the extract, a method for preparing the extract and a method for preventing and treating plant diseases.

The invention relates to a bactericidal composition containing zoxamide and fludioxonil, the effective components of the bactericidal composition are binary compound of zoxamide and fludioxonil, the balance is auxiliary materials, and the bactericidal composition can be prepared into various dosage forms, can be used for preventing and treating various diseases on crops such as vegetables and fruit trees, and can be applied to prevention and treatment of various diseases on crops such as vegetables, fruit trees and the like. The bactericidal composition is especially suitable for preventing and treating gray mold of crops such as flowers, vegetables, rice, wheat, fruit trees, cotton and oilseed rape, and preventing and treating phytophthora diseases of crops such as solanaceae, liliaceae and cucurbitaceae, such as pepper phytophthora blight and potato late blight. Compared with a single agent, the synergistic bactericidal composition disclosed by the invention has the advantages that the prevention and treatment effects on gray mold, epidemic disease and other diseases of crops are improved, the dosage can be greatly reduced, the environmental pollution is reduced, and the generation of drug resistance of germs is overcome and delayed.