Botanical insecticide and preparation method thereof
A plant-derived insecticide and insecticide technology, applied in the field of plant-derived insecticides for pest control and its preparation, can solve problems such as environmental pollution, ecological balance destruction, food safety, etc., and achieve no secondary pollution , High insecticidal activity, good economical effect
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Embodiment 1
[0015] Example 1 The preparation of the extract of penicillin petroleum ether
[0016] Weigh 1.82kg of dry Herba chinensis, grind it into fine powder, put it in a round bottom flask, then add ethanol solution with a volume concentration of 68%, soak for 30min, heat and reflux for extraction 3 times, the ratio of solid to liquid is 1:12 , 1:8 and 1:6 (kg / L), extract for 2.5 hours for the first time, extract for 2 hours for the second and third times, filter, combine the three extractions, centrifuge at 2000rpm, and concentrate the supernatant by rotary evaporation at a temperature of 45°C , vacuum degree 0.1MPa, until there is no smell of ethanol, add 600mL of purified water to dissolve the suspension, extract 3 times with an equal volume of petroleum ether, combine the petroleum ether extracts, concentrate to remove the solvent, and crush, that is, the extraction of penicillin petroleum ether things.
Embodiment 4
[0021] Example 4 The control test of the extract of Herba chinensis on the control of cotton bollworm
[0022] The extract powders prepared in Example 1 and Comparative Examples 1-2 were respectively added to the nutrient powder of cotton bollworm at a rate of 0.2‰, and then 30 second-instar larvae of cotton bollworm were inserted into each group and placed in a breeding room for rearing. The feeding conditions are temperature 25-28°C, RH 70%-80%, light as L:D=14h:10h. Cultivate 24, 48 hours to observe the death situation of cotton bollworm. The method of judging the death of pests: touch the larvae with the tip of a brush, if the body does not move, it is considered dead. As a result, the death conditions of the second instar cotton bollworm in each group are shown in Table 1. According to the test results in Table 1, it can be seen that the ethyl acetate extract of chrysanthemum has no killing effect on cotton bollworm, the alcohol extract of chrysanthemum has little kil...
Embodiment 5
[0025] Example 5 The control test of Plutella xylostella extracts from C.
[0026] The extract powders of Plutella xylostella prepared in Example 1 and Comparative Examples 1-2 were added to Plutella xylostella nutrient powder at a rate of 0.2‰, and then 30 second-instar larvae of Plutella xylostella were inserted into each group and placed in a breeding room for rearing. The feeding conditions are temperature 25-28°C, RH 70%-80%, light as L:D=14h:10h. Cultivate for 24 and 48 hours to observe the death of the second instar diamondback moth. The method of judging the death of pests: touch the larvae with the tip of a brush, if the body does not move, it is considered dead. The mortality of the second instar diamondback moth in each group is shown in Table 2. According to the test results in Table 2, it can be seen that the ethyl acetate extract of P. xylostella has no killing effect on Plutella xylostella, the ethanol extract of P. xylostella has little killing effect on P....
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