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Plant extract for controlling parasitic nematodes

A technology for extracts and plants, applied in the field of preventing and controlling plant diseases, extracts of cucurbit plants or their parts, can solve problems such as harmful gene modification, and achieve the effect of preventing damage

Pending Publication Date: 2022-03-04
UNIV GENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They may also act as mutagens and / or carcinogens to cause irreversible and deleterious genetic modifications

Method used

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  • Plant extract for controlling parasitic nematodes
  • Plant extract for controlling parasitic nematodes
  • Plant extract for controlling parasitic nematodes

Examples

Experimental program
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Effect test

preparation example Construction

[0047] A typical (fresh) peel extract preparation process involves washing the fruits as deemed necessary and peeling them manually or mechanically. These skins are then collected and blended, mixed or ground to obtain a slurry. In one embodiment, the blending or mixing is preferably continued for at least 1 or 2 minutes, especially 2 to 10 minutes.

[0048] The solvent used in the process of preparing the plant extract is preferably selected from the group consisting of ethanol, methanol, hexane, water or any suitable buffer such as for example an aqueous based buffer with a pH range of 5 to 8 (eg, sodium phosphate buffer, potassium phosphate buffer, Tris buffer, phosphate buffered saline, or borate buffered saline), including combinations thereof. In a specific embodiment, the solvent is water.

[0049] Different types of extraction methods are possible. In one embodiment, the at least Extraction step (mixing and optionally sieving). This is also known as a cold extract...

Embodiment 1

[0131] Example 1: Using fresh material from different parts of the squash (Cucurbita moschata) 'Muscat' Prepared cold extracts: aerial application to rice Root-knot nematode Meloidogyne graminicola Systemic Defense Activation Experiment

[0132] In this experiment, the systemic defense-inducing effect of five cold plant extracts from different fresh plant parts of squash 'Muscat' was investigated in rice against the root-knot nematode Meloidogyne graminearum by inoculating roots with the nematode The cold extract was sprayed on the shoot of rice for 24 hours. Root galls were counted after 14 days and plant length was measured. The number of ovipositing females in the infected root system was evaluated as a measure of nematode development. The results showed that cold extracts of fresh material from different plant parts of squash 'Muscat' all had systemic defense-inducing activity against root-knot nematodes, in which the number of galls per plant was significantly ...

Embodiment 2

[0133] Embodiment 2: to rice Systemicity of the root-knot nematode Meloidogyne graminicola defense activation experiment

[0134] In this experiment, skins (Cucurbitaceae Peel Extract: COPE) from different plants of the Cucurbitaceae family (including cucumber, squash 'Muscat' and zucchini) were sprayed 24 hours before root-knot nematode inoculation on the roots. to the above ground. Nematode development was assessed by dividing nematodes in the interior of the root tissue into four classes: second stage larvae (J2), developing larvae (J3 / J4), juvenile females and ovipositing females. No significant effects on shoot or root growth were observed ( figure 2 a). For all extracts, the number of galls was reduced by approximately 50% ( figure 2 b). A strong negative effect on nematode development in rice roots was observed, mainly for zucchini and pumpkin 'Muscat' extracts, in which few egg-laying females were found in rice roots ( figure 2 c).

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Abstract

The present invention is in the field of agricultural pathogen control by inducing systemic plant defense. More specifically, the present invention relates to an extract of a plant belonging to the family Cucurbitaceae and its use for protecting plants from plant pathogens, in particular parasitic nematodes. The invention also provides a composition containing the extract, a method for preparing the extract and a method for preventing and treating plant diseases.

Description

technical field [0001] The present invention is in the field of agricultural pathogen and pest control. [0002] More specifically, the present invention relates to extracts of plants of the family Cucurbitaceae or parts thereof, and their use for protecting plants from diseases and harmful organisms, especially parasitic nematodes. The present invention also provides a composition comprising the extract, a method for preparing the extract and a method for controlling plant diseases. Background technique [0003] Plants are affected by a wide variety of possible pathogens, including phytopathogens, fungi, oomycetes, mites, insects and plant-parasitic nematodes, which cause significant economic losses in agriculture. [0004] Bacteria are prokaryotic unicellular microorganisms, usually in the size range of 1-2 μm, which are present on all plant surfaces (epipytes) and inside plants (endophytes), where they can appear beneficial or pathogenic to the host. Phytopathogenic bac...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N25/06A01P5/00A01C1/06
CPCA01N65/00A01N65/08A01N25/06A01C1/06A01P5/00A01N25/02A01N25/30
Inventor 蒂娜·金德特玛艾可·佩尼尔乔纳斯·德基泽尔伊娃·德格鲁特
Owner UNIV GENT
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