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A method for preventing and controlling melon bacterial fruit spot and its special seed fungicide

A seed and seed treatment technology, applied in biocides, seed and rhizome treatment, botany equipment and methods, etc., can solve the problems of limited seedling disease prevention and control effects, affecting germination rate, difficulty in seed treatment, etc.

Active Publication Date: 2022-05-17
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacteria stored inside the seeds have high stress resistance, longer survival time, and stronger ability to spread diseases. At the same time, the bacteria stored inside the seeds make it difficult to detect and treat the seeds
At present, the seed transfer to prevent watermelon bacterial fruit spot is mainly through post-harvest fermentation and treatment of seeds with hydrochloric acid, Physan 20, Tsunami100, etc., but the treatment affects the germination rate, especially for triploid seedless watermelon seeds
Although the soaking of dry seeds in warm soup and treatment with copper preparations can reduce the incidence of seedling disease, they cannot completely remove pathogenic bacteria. Relying on agricultural antibiotics and copper preparations has limited effect on disease control at seedling stage, and it is easy to lead to the emergence of bacterial resistance

Method used

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  • A method for preventing and controlling melon bacterial fruit spot and its special seed fungicide
  • A method for preventing and controlling melon bacterial fruit spot and its special seed fungicide
  • A method for preventing and controlling melon bacterial fruit spot and its special seed fungicide

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Effect test

Embodiment 1

[0058] Example 1. Screening of small molecular compounds and their application in the prevention and control of bacterial fruit spot of melons

[0059] 1. Pathogenicity experiment of watermelon acidophilus strains

[0060] 1. The strain preservation solutions of 12 watermelon acidophilus bacteria shown in Table 1 (bacterial strains are all stored in 15% (v / v) glycerol aqueous solution, -80 ° C) are inoculated in PF medium or NBY medium respectively, 28 ° C Culture for 2-3d (purpose for activation).

[0061] 2. After completing step 1, respectively expand the activated watermelon acidophilus to obtain a concentration of 10 8 CFU / mL bacterial suspension.

[0062] 3, get watermelon seed, conventional planting, obtain the watermelon seedling that grows two true leaves. Get melon seeds, conventional planting, obtain the melon seedling that grows two true leaves.

[0063] 4. Dip the sterile dissecting scissors (obtained by heating the dissecting scissors on a flame or soaking th...

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Abstract

The invention discloses a method for preventing and controlling bacterial fruit spot disease of melons and a special seed fungicide. The method is to soak the seeds with Nafloxacin. Experiments have proved that Nafloxacin can effectively remove the watermelon acidophilus bacteria outside and inside the seeds of cucurbit crops (such as watermelon and muskmelon), and can be absorbed by the seedlings during seed germination, reducing the watermelon acidophilus bacteria seed-seedling can effectively inhibit the incidence of emergence of seeds carrying watermelon acidophilus, and achieve the purpose of preventing and controlling bacterial fruit spot of melons; at the same time, nafloxacin does not affect seed germination and seedling growth; nafloxacin also inhibits The growth of pathogenic bacteria (such as tomato canker, cruciferous black rot fungus) has no significant effect on the growth of plant growth-promoting rhizobacteria (such as Pseudomonas fluorescens, Bacillus subtilis). Nafloxacin can be used as a seed fungicide for production and promotion. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preventing and controlling bacterial fruit spot of melons and a special seed fungicide thereof. Background technique [0002] Bacterial fruit blotch (BFB for short) of melons, also known as bacterial fruit rot, is a devastating disease that can cause production reduction or even failure of watermelons and melons, and is also a worldwide quarantine disease. BFB is caused by Acidovorax citrulli, which has the characteristics of rapid onset, rapid transmission, strong explosive power, and severe damage. Agricultural production safety poses a serious threat. Since the outbreak of melon bacterial fruit spot disease in watermelon fields in the United States in the 1990s, the disease has spread rapidly in many countries around the world in the past two decades, mainly due to the seed transmission of watermelon acidophilus and Related to the increasingly frequent i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P1/00A01C1/00A01G13/00
CPCA01C1/00A01G13/00A01N43/90
Inventor 徐秀兰芦钰张海军盖瑞仕拉贾塞卡拉莎莉米勒
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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