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Compound microbial agent and application thereof in preventing and treating various plant diseases

A technology of compound microbial bacterial agent and bacterial agent, which is applied in the biological field

Active Publication Date: 2020-06-16
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fungicides currently reported are often only aimed at a single disease, and the occurrence of plant diseases is often caused by a variety of pathogenic bacteria. At the same time, there are still few reports on the biocontrol fungicides for a variety of major root diseases.

Method used

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  • Compound microbial agent and application thereof in preventing and treating various plant diseases
  • Compound microbial agent and application thereof in preventing and treating various plant diseases
  • Compound microbial agent and application thereof in preventing and treating various plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116]Example 1, Isolation, Identification and Preservation of NMB1, NMB3, NMB4, and XXB-24 Strains

[0117] (1) Isolation, identification and preservation of NMB1, NMB3 and NMB4 strains

[0118] 1. Isolation of NMB1, NMB3, and NMB4 strains

[0119] NMB1, NMB3, and NMB4 strains were all isolated from the rhizosphere soil in the sugar beet continuous cropping plots in Ulanqab City, Inner Mongolia Autonomous Region, where the sugar beet root rot was severe. The specific separation method is as follows: pick the rhizosphere soil collected from the sugar beet continuous cropping plot, pick the root system, scrape the rhizosphere soil, weigh 5g, put it into the prepared sterilized triangular flask under aseptic conditions, and wash it with sterilized water. Serial dilution to 10 -6 times, set 3 repetitions. Take 200 μL of the soil samples of gradient dilution and apply them on the PDA medium plate respectively, cultivate them in a 28°C incubator, observe the growth status of the...

Embodiment 2

[0144] Embodiment 2, the preparation of composite microbial bacterial agent

[0145] 1. Preparation of seed solution

[0146] Bacillus thuringiensis NMB1, Bacillus amyloliquefaciens NMB3, Bacillus megaterium NMB4, Burkholderia pyrrole XXB-24, Acinetobacter oilophilus NMB17 and Pseudomonas fluorescens pf27 obtained in Example 1 were inoculated in liquid respectively. In the medium (NMB1, NMB3, NMB4, XXB-24 and NMB17 strains were inoculated in LB liquid medium, and pf27 strain was inoculated in KB liquid medium), shake culture at 28°C and 200rpm for 12-16h, respectively to make NMB1 Seed solution, NMB3 seed solution, NMB4 seed solution, XXB-24 seed solution, NMB17 seed solution and pf27 seed solution.

[0147] 2. Preparation of cell pellet

[0148] The NMB1 seed solution, NMB3 seed solution, NMB4 seed solution, XXB-24 seed solution, NMB17 seed solution and pf27 seed solution obtained in step 1 were added to the liquid medium (NMB1 seed solution, NMB3 seed solution, NMB4 seed ...

Embodiment 3

[0157] Embodiment 3, the field test of composite microbial bacterial agent

[0158] 1. Field test method

[0159] Compound bacterial agent treatment group (Zhongke 303): before transplanting tobacco seedlings, dip the tobacco seedlings with 2L of the compound microbial bacterial agent prepared in Example 2 for 30 minutes, and transplant after treatment.

[0160] Control group (CK): Before the tobacco seedlings were transplanted, the tobacco seedlings were dipped in 2 L of water for 30 minutes, and transplanted after the treatment.

[0161] Three biological repetitions were performed for each treatment, and 60 tobacco seedlings were transplanted for each biological repetition. Each biological replicate of the compound bacterial agent treatment group and the control group was arranged at intervals.

[0162] 2. Inhibitory effect of compound microbial agent Zhongke 303 on tobacco disease

[0163] 1. Quantitative analysis of field pathogens

[0164] Tobacco rhizosphere microbia...

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Abstract

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a composite microbial bacterial agent and its application, in particular to a composite microbial bacterial agent and its application in preventing and treating various plant diseases. Background technique [0002] Driven by limited cultivated land resources and market economic interests, the area of ​​continuous cropping and multi-cropping continuous cropping is increasing day by day, increasing the obstacles to continuous cropping. The crops with continuous cropping obstacles are mainly Solanaceae crops (peppers, tomatoes, tobacco, etc.), and melons, beans, and medicinal plants such as Panax notoginseng, which use their roots as medicine, are all outstanding. Continuous cropping obstacles are a complex and difficult to solve history It has long been the focus and difficulty to be overcome in the development of agriculture at home and abroad. Tobacco is an impor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N63/20A01N63/23A01P1/00A01P3/00A01P21/00C12R1/07C12R1/11C12R1/01C12R1/39
CPCC12N1/20A01N63/00C12R2001/11C12N1/205C12R2001/39C12R2001/07C12R2001/01
Inventor 叶健姚香梅尹策策
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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