51results about How to "Reduce virus content" patented technology

The invention relates to a super-sensitivity method, primer group and kit for capturing whole genome of novel coronavirus. The method can conveniently and quickly amplify the whole genome of the novelcoronavirus by using a small amount of RNA, and can directly dock with a second-generation sequencing library-building reagent and a third-generation sequencing platform so as to obtain a whole genome sequence of the novel coronavirus.

The invention discloses a genetic engineering subunit vaccine for a porcine circovirus as well as a preparation method and application of the genetic engineering subunit vaccine. By cloning the nucleocapsid protein of the novel porcine circovirus 3 (PCV3), a PCV-Cap protein with higher purity is successfully expressed by using an escherichia coli or baculovirus expression system. The subunit vaccine for the PCV3 is successfully developed for the first time by using the PCV-Cap protein; the prepared vaccine is high in antigen purity, good in safety and strong in immunogenicity, and has no pathogenicity to pigs and other animals; the antigen has good solubility in a neutral PH buffer solution; furthermore, the preparation method is simple and low in cost, thus being suitable for large-scaleindustrial production; an effective and powerful means is provided for the prevention and control of novel PCV, and the genetic engineering subunit vaccine has a wide application prospect in the fieldof the prevention and control of the PCV3.

The invention belongs to the technical field of animal virology and immunology, in particular to a double-antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting Japanese encephalitis virus antigens in swine, human and mosquitoes. Core reagents of the kit comprise a Japanese encephalitis virus E protein monoclonal antibody serving as a primary antibody and a rabbit polyclonal antibody serving as a secondary antibody. The invention discloses a preparation and purification method of the Japanese encephalitis virus E protein monoclonal antibody and the rabbit polyclonal antibody. The invention also discloses a detection method of the double-antibody sandwich ELISA. A hybridoma cell strain secreting the monoclonal antibody is preserved in China Center for Type Culture Collection with CCTCC NO. C2010114.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention provides a high-pollution building space exhaust disinfection and purification system and method. The system comprises a pollution region negative pressure metal ventilation pipe, and any position of the pollution region negative pressure metal ventilation pipe is communicated to the inlet end of a high-resistance ventilation pipeline with a heat insulation and insulation layer. Theoutlet end of the high-resistance ventilation pipeline with the heat insulation and insulation layer is connected to the inlet end of an outdoor negative pressure metal ventilation pipe through an electric backflow prevention valve, and the outlet end of the outdoor negative pressure metal ventilation pipe is connected to an extraction opening of an efficient frequency conversion draught fan. A plurality of virus killing devices are sequentially arranged at the rear end of the efficient variable-frequency draught fan. By means of the system and method, air possibly containing viruses in a high-pollution building space is subjected to high-temperature disinfection and sterilization through an electric heating pipeline, then the air subjected to high-temperature disinfection and sterilization is guided to an outdoor multi-virus disinfection and sterilization device through the negative pressure pipeline to conduct diversified thorough disinfection and sterilization on the introduced air,and the viruses in the air in the high-pollution building space can be thoroughly removed.

The invention relates to real-time fluorescent quantitative RT-PCR detection primers for a serotype of a Palyam serogroup virus (PALV), probes and a detection kit and belongs to the technical field ofmolecular biological detection on animal viruses. The kit comprises 3 pairs of primers and further comprises three probes, a negative control template, positive control templates, standard substancetemplates and a PCR amplification reagent. RNase-free water serves as the negative control template; the positive control templates are CHUV, BCV and DAV inactivated viruses separately; and the standard substance templates are gene segment 2 single-stranded RNAs of CHUV, BCV and DAV. The kit disclosed by the invention can be used for detecting three kinds of serotype PALVs popular in China and hasthe advantages of specificity, sensitivity, rapidness, high efficiency and the like; and a standard curve established by utilizing the standard substance templates in a matched manner can be used forcarrying out quantitative detection on RNAs of all PALV serotype strains in clinical samples, and thus, the working efficiency is increased greatly.

The invention discloses a buried four-station-in-one purification treatment device for medical waste. The device comprises a household garbage throwing area, a medical waste classifying and packagingarea, a sludge lifting and drying treatment area, a foul gas treatment area and a sewage treatment area, wherein the household garbage throwing area is arranged at the upper end of the right side of the purification treatment device and is used for packaging household garbage; the medical waste classifying and packaging area is arranged on the left side of the household garbage throwing area and used for classifying and packaging metal, nonmetal and plastic garbage; the sludge lifting and drying treatment area and the foul gas treatment area are both arranged on the right side of the medical waste classifying and packaging area, and the foul gas treatment area is arranged on the front side of the sludge lifting and drying treatment area; and the sewage treatment area is arranged below thepurification treatment device and is used for collecting waste liquid and waste gas. According to the device, a medical waste gas station, a waste water station and a solid waste station which carry germs and viruses are integrated in the same device, so comprehensive treatment of waste gas, waste water and solid waste is realized; and the device has the advantages of reducing intermediate links,improving the medical environment and facilitating implementation and management.

The invention discloses a device for preparing a fertilizer by using excess sludge and agricultural and forestry waste. The device comprises a mixing system, a fermentation composting system, a screening system, a granulation and drying system, an exhaust gas treatment system, and a wastewater backflow system. Compared with the prior art, by using water, aerobiotic microorganisms and anoxic microorganisms in the excess sludge and cellulose in the agricultural and forestry waste, the device achieves organic matter degradation, pathogenic bacterium killing and heavy metal passivation of the excess sludge and the agricultural and forestry waste through the aerobiotic fermentation, anoxic fermentation and anaerobic fermentation of the excess sludge and the agricultural and forestry waste; the obtained product is thorough in degradation, good in air permeability, high in humus content, comprehensive and rich in nutrient and low in bacterium and virus content, and can serve as a farmland fertilizer or a soil conditioner. The device is simple in structure, stable and reliable in operation, high in production efficiency, and free of odor gas and secondary pollution.

The invention belongs to the field of biotechnology, and discloses a preparation method and application of specific antibody containing milk or serum produced by novel coronavirus immunized cows. Theinactivated novel coronavirus hCov-19 / Wuhan / ZY38-1 / 2020 is used for immunizing dairy cows, and a large amount of immune milk and serum can be obtained. Both the immune milk and the serum contain highcontent of antibodies, viruses can be effectively neutralized and stopped from invading cells, and the significant anti-SARS-CoV-2 virus effect is achieved; after Igs protein purified from immune milkis injected into mice, the mortality of novel coronavirus infected mice can be significantly reduced, the influence of novel coronavirus infection on weight loss of the mice is reduced, and the content of viruses in lung tissue of the mice is reduced. The immunized milk and serum can be used as sources of antibodies for preparations for preventing and treating novel coronavirus pneumonia, and thelimitation of the sources of plasma for rehabilitation of novel coronavirus pneumonia.

The invention discloses a heat-proof freeze-dried protective agent and a preparation method and application thereof. The heat-proof freeze-dried protective agent is prepared by mixing a solution A and a solution B according to the volume ratio of 1:1, wherein the solution A comprises 0.01-0.03 g / ml of trehalose, 0.01-0.04 g / ml of sodium thiosulfate, 0.01-0.02 g / ml of casein enzymatic hydrolysate, 0.01-0.02 g / ml of thiocarbamide, 0.005-0.02 g / ml of 199 culture medium and 0.001-0.01 g / ml of O-2'-hydroxypropyl trimethyl ammonium chloride chitosan, is dissolved and determined in volume by using a polyvinyl pyrrolidone (PVPK30) water solution with the mass percent concentration of 2%-6%, and is disinfected by using 0.22 micrometer filter; the solution B contains 0.01-0.03 g / ml of gelatin, 0.05-0.1 g / ml of peptone and 0.05-0.1 g / ml of sucrose, is dissolved and determined in volume by using deionized water, and is autoclaved for later use. Compared with the existing product, the improved heat-proof freeze-dried protective agent disclosed by the invention can increase the protection duration of a product, furthest maintain the virus titer and shorten the freeze-drying time, and is long in antibody duration time and favorable to use in industrialized large-scale production.

The invention discloses a nested RT-PCR method and a primer combination for detecting potato mop-top virus. The method comprises the following steps: firstly, conducting reverse transcription by virtue of a random primer by taking RNA as a template, so as to obtain cDNA; secondly, taking the cDNA as a template; and thirdly, conducting the second turn of PCR by taking the fragment 1 of the first PCR amplified product as a template, wherein by virtue of two turns of the PCR reactions, the specificity and the sensitivity of detecting and identifying the virus are improved. Therefore, the nested RT-PCR method, when used for detecting the PMTV, is high in virus detection sensitivity and strong in specificity.

The invention discloses a temporary emergency hospital ventilation system and method. The temporary emergency hospital ventilation system comprises a negative pressure ventilation system and a medicalstaff ventilation protection system, wherein the negative pressure ventilation system comprises a first air supply pipe communicating with a medical care corridor and a ward area; and the medical staff ventilation protection system is arranged in the ward area, communicates with the outlet end of the first air supply pipe and is used for tracking and positioning the range or coordinates of the position where a human body is located in the ward area and guiding the air outlet direction of the first air supply pipe to point to the range or coordinates of the position where the human body is located. The temporary emergency hospital ventilation system has the beneficial effects that the medical staff ventilation protection system is arranged at the outlet end of the first air supply pipe ofthe negative pressure ventilation system, so that medical staff in the ward area and surrounding air of the medical staff are always affected by flowing airflow, the content of viruses in the surrounding air of the medical staff can be effectively reduced, and therefore the safety of the medical staff in the ward is effectively protected.

The invention discloses a method for reducing tomato yellow leaf curl virus content in bemisia tabaci by utilizing insecticide fungi isaria javanicus. In order to solve the problem that the tomato yellow leaf curl virus content in bemisia tabaci is not reduced for promoting the disease control effect, the insecticide fungi isaria javanicus is utilized to treat the bemisia tabaci and the tomato yellow leaf curl virus content in the bemisia tabaci can be obviously reduced, so that the capacity of transmitting virus of the bemisia tabaci can be weakened and the promotion for the control effect for the virus and disease is benefited. Compared with the traditional methods in production, for example, utilizing insect resistant variety, controlling the bemisia tabaci quantity and utilizing virus antagonist to prevent and control the tomato yellow leaf disease, the method disclosed by the invention has the advantages that the insecticide fungi isaria javanicus is utilized to reduce the tomato yellow leaf curl virus content in the bemisia tabaci so as to promote the virus control effect, the disease control mode is novel, the safety is high and the method has a certain application prospect.

The invention relates to a heat treatment detoxification method for sweet potatoes. The heat treatment detoxification method specifically comprises the following steps: (1) preferably selecting sweet potato seedlings; (2) pretreating the sweet potato seedlings; and (3) detoxifying the sweet potato seedlings. Proved by experiments, detoxified sweet potato seedlings obtained by using the heat treatment detoxification method for sweet potatoes, provided by the invention, are better in detoxification effect, and the yield per unit area reaches up to more than 2940kg, while the yield per unit area of the planted detoxified seedlings obtained by carrying out stem tip tissue culture on detoxified stem tips obtained by using the existing heat treatment detoxification method for sweet potatoes by using the same method is only 2258kg; and meanwhile, the method disclosed by the invention is shorter in time consumption and better in detoxification effect; and the existing heat treatment detoxification method often takes 2-4 weeks or even longer, while the method disclosed by the invention only takes about 10 days.

The invention provides a system and method for exhausted air sterilization and aeration and comprehensive heating disinfection of a highly-contaminated building space. The system comprises a contaminated area negative pressure metal ventilation pipe, any position thereon communicates with an inlet end of a high-resistance thermal-insulation-layer-carried ventilation pipeline, an outlet end of thehigh-resistance thermal-insulation-layer-carried ventilation pipeline is connected to an inlet end of an outdoor negative pressure metal ventilation pipe through an electric anti-backflow valve, and an outlet end of the outdoor negative pressure metal ventilation pipe is connected with an exhaust port of a high-efficiency variable-frequency fan. Various virus killing devices are sequentially disposed at the rear end of the high-efficiency variable-frequency fan. The system disinfects the air that may contain viruses in the highly-contaminated building space at high temperature through an electric heating pipeline and then guides the air that is disinfected at high temperature to the various outdoor virus killing devices through negative pressure pipelines to diversely and thoroughly disinfect the guided air, thereby thoroughly removing viruses in the air in the highly-contaminated building space.

The invention discloses a prescription, which contains aqueous extracts of shiitake mushrooms, poria cocos, zingiber officinale and dried tangerine peels and plays a role in resisting H1N1 viruses, and application thereof. The prescription is mainly made up of the shiitake mushrooms, the poria cocos, the zingiber officinale and the dried tangerine peels. Proven by antiviral tests, the prescription disclosed by the invention can be used for remarkably improving the immunologic function of H1N1 virus infected mice and improving the pneumonia pathological change degree of the H1N1 virus infected mice and can be used for remarkably improving the specific antibody level in blood serum of the H1N1 virus infected mice, remarkably lowering the content of viruses in lungs of the H1N1 virus infected mice and improving the level of lung cell factors IL-2, so that the prescription has a good H1N1 influenza virus resisting action.

The invention provides an exhaust aeration disinfection and sterilization system and method for a high-pollution building space. The system involves a polluted area negative pressure metal ventilationpipe, wherein any position of the polluted area negative pressure metal ventilation pipe communicates with the inlet end of a high-resistance ventilation pipeline with a thermal isolation and insulation layer, and the outlet end of the high-resistance ventilation pipeline with the thermal isolation and insulation layer is connected to the inlet end of an outdoor negative pressure metal ventilation pipe through an electric backflow prevention valve; and the outlet end of the outdoor negative pressure metal ventilation pipe is connected to an extraction opening of an efficient variable-frequency fan, and a plurality of virus disinfection and sterilization devices are sequentially arranged at the rear end of the efficient variable-frequency fan. According to the exhaust aeration disinfectionand sterilization system and method for the high-pollution building space, air possibly containing viruses in the high-pollution building space is subjected to high-temperature disinfection and sterilization through an electric heating pipeline firstly, then the air subjected to high-temperature disinfection and sterilization is guided to the outdoor through a negative pressure pipeline, and theplurality of virus disinfection and sterilization devices carry out diversified thorough disinfection and sterilization on the introduced air so that the viruses in the air in the high-pollution building space can be thoroughly removed.

The invention provides a crRNA molecule and a method for detecting D614G mutation in SARS-CoV-2 by using CRISPR-Cas12a. The method for detecting D614G mutation in SARS-CoV-2 by using the CRISPR-Cas12a technology through the crRNA is simple, convenient, easy to implement, rapid and specific; when a recombinase polymerase nucleic acid amplification technology is combined, the sensitivity is 100 copies / [mu]L, the sensitivity is extremely high, compared with a common gene sequencing method, the method can achieve the identification of a sample with lower virus content, and is suitable for large-scale screening.

The invention provides an exhaust multi-process comprehensive disinfection and sterilization system and method for a high-pollution building space. The system involves a polluted area negative pressure metal ventilation pipe, wherein any position of the polluted area negative pressure metal ventilation pipe communicates with the inlet end of a high-resistance ventilation pipeline with a thermal isolation and insulation layer, and the outlet end of the high-resistance ventilation pipeline with the thermal isolation and insulation layer is connected to the inlet end of an outdoor negative pressure metal ventilation pipe through an electric backflow prevention valve; and the outlet end of the outdoor negative pressure metal ventilation pipe is connected to an extraction opening of an efficient variable-frequency fan, and a plurality of virus disinfection and sterilization devices are sequentially arranged at the rear end of the efficient variable-frequency fan. According to the exhaust multi-process comprehensive disinfection and sterilization system and method for the high-pollution building space, air possibly containing viruses in the high-pollution building space is subjected to high-temperature disinfection and sterilization through an electric heating pipeline firstly, then the air subjected to high-temperature disinfection and sterilization is guided to the outdoor througha negative pressure pipeline, and the plurality of virus disinfection and sterilization devices carry out diversified thorough disinfection and sterilization on the introduced air so that the virusesin the air in the high-pollution building space can be thoroughly removed.

The invention discloses a planting method for saffron crocus tissue culture micro bulbotubers, and technical service is provided for seedling hardening planting in the later period of saffron crocus plant tissue culture. The planting method for saffron crocus tissue culture micro bulbotubers includes the following steps of seed source preparing, wherein seed selecting and sterilizing are involved; soil preparation, wherein the substeps of planting in a field, applying base fertilizer, disinfecting soil and carrying out furrow opening and ridging are included; planting and observing, wherein two substeps of sowing and observing are included. As the bulbotubers and soil are sterilized and disinfected by adopting multiple disinfecting methods, damage to the tissue culture micro bulbotubers by microorganisms is eliminated or reduced, and the planting survival rate is large; the method of two-time deep ploughing is adopted, and the soil is loosened, which is more beneficial for growth of saffron crocus. The planting method is easy to operate and low in cost, and large-scale industrial production is easier to achieve.

The invention discloses a negative-pressure disinfection infusion consulting room for the pneumology department, and relates to the field of medical disinfection. The negative-pressure disinfection infusion consulting room for the pneumology department comprises a PLC, a negative pressure device, disinfection devices and an infusion operation table which are arranged in the consulting room, wherein the disinfection devices are arranged at a consulting room entrance disinfection area and the infusion operation table correspondingly, the consulting room entrance disinfection area is provided with an entrance and an exit, the entrance and the exit are both sealed by induction doors, and the entrance of the consulting room entrance disinfection area and the entrance of the consulting room arethe same entrance. The negative pressure device and the disinfection devices are controlled to be started or stopped by adopting a logic command preset by the PLC, it is effectively ensured that a specific disinfection scheme is adopted in a specific condition in the area, so that viruses are effectively prevented from being spread outwards from the consulting room, and meanwhile, medical personnel and patients can be well protected.

The invention belongs to the technical field of biology and discloses a novel coronavirus, a preparation method for specific-antibody-containing milk or serum produced by a dairy cow immunized by the novel coronavirus and application of the specific-antibody-containing milk or serum. A large volume of immunized milk and serum can be obtained through immunizing the dairy cow by using an inactivated novel coronavirus SARS-CoV-2 / ZY38-1, both the immunized milk and the serum contain high-content antibodies, viruses can be effectively neutralized, the viruses are prevented from invading cells, and the immunized milk and serum have an obvious SARS-CoV-2 virus resisting effect; and after an Igs protein purified from the immunized milk is injected into a mouse, the death rate of the mouse infected by the new coronavirus can be remarkably reduced, the influence of new coronavirus infection on the weight loss of the mouse is reduced, and the content of viruses in lung tissue of the mouse is lowered. The immunized milk and serum provided by the invention can be used as an antibody source of preparations for preventing and treating novel-coronavirus pneumonia, and the limitation of sources of rehabilitation blood plasma for the novel-coronavirus pneumonia is made up.

The invention relates to an RPA-LFD primer, a probe and a kit for jointly detecting an epidemic hemorrhagic disease virus and a Palimer serum group virus, and belongs to the technical field of molecular biological detection of animal viruses. The primer and the nfo probe for jointly detecting EHDV and PALV are designed, the RPA-LFD detection kit capable of jointly detecting the nucleic acids of EHDV and PALV popular in China is constructed on the basis, and the kit has the advantages of specificity, sensitivity, rapidness, high efficiency, isothermal amplification, on-site rapid diagnosis and the like, and is easy to popularize and apply.

The invention relates to the technical field of sewage treatment, aims to the problem that municipal wastewater is hard to recycle or use after common physical filtering, and provides a municipal wastewater treatment method. According to the technical scheme, the method comprises the following steps: S1, performing primary filtering so as to obtain filtered water; S2, adding a flocculant into thefiltered water so as to obtain a flocculant mixed liquid; S3, heating the flocculant mixed liquid till boiling; S4, performing ultraviolet radiation, and performing sterilization and disinfection through vapor; S5, performing condensation; and S6, performing secondary filtering, wherein the flocculant comprises 100 parts of polyacrylamide, 20-25 parts of methylcellulose, 5-10 parts of casein and 0.01-0.02 part of 2-amino isonicotinic acid. The flocculant mixed liquid is heated till boiling to kill a part of bacteria, the vapor is radiated through an ultraviolet lamp, then a good permeation effect is achieved, a very good ultraviolet sterilization and disinfection effect can be achieved, the content of bacterial viruses in final pure water can be greatly reduced, a good sewage treatment effect can be achieved, the treated pure water can be recycled, waste source waste can be reduced, and meanwhile, pollution to the environment and water resources can be reduced.

The invention relates to micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof and provides structure of the micromolecular polypeptide.The micromolecular polypeptide has the advantages that the micromolecular polypeptide is low in synthesis cost, convenient safe to use and capable of effectively inhibiting proliferation of swine fever virus and can be used with vaccines, thereby greatly improving vaccine antibody valence, prolonging time of antigen in vivo and prolonging immune protection period of vaccines.

The invention belongs to the field of biological agents, and discloses application of R848 in preparation of a medicine for inhibiting novel coronavirus SARS-CoV-2, an applicant finds that R848 has asmall toxic effect on Caco-2 cells through an in vitro cell test and a mouse test, infection of the Caco2 cells by the SARS-CoV-2 can be remarkably inhibited at the in vitro cell level, and proliferation of the Caco-2 cells is inhibited. The characteristics of safety and high efficiency are realized; meanwhile, by constructing a mouse SARS-CoV-2 infection model, it is found that R848 can significantly reduce the weight loss degree of the mouse, reduce the virus content in the lung tissue of the mouse and improve the survival rate of the mouse, so that R848 has very large clinical treatment potential.

The invention belongs to the field of biological preparations, and in particular relates to the application of 3-indoleacetonitrile in the preparation of drugs for inhibiting novel coronavirus SARS-CoV-2. The present invention finds for the first time that 3-indoleacetonitrile can inhibit the new coronavirus at the cell and animal levels, and can relieve the body's adverse symptoms caused by the new coronavirus infection. 2. It is effective in the infection model, which can significantly reduce the virus content in the lung tissue of mice, reduce the degree of weight loss of mice, and improve the survival rate of mice, which makes 3-indoleacetonitrile have great potential for clinical treatment.