This invention provides a method for creating rice materials that can block the transmission of
rice stripe virus by planthoppers, belonging to the field of
agricultural science and technology. Based on the characteristic that the
rice stripe virus (RSV)
glycoprotein NSvc2-N mediates
viral infection of the
planthopper midgut, NSvc2-N is selected from this
glycoprotein. S (720 bp, encoding the soluble region of NSvc2-N, i.e., amino acids 30-268), was integrated into the rice
genome using
transgenic technology. The resulting transgenic
rice plants significantly reduced the
virus acquisition rate,
virus content in the insects, and
virus transmission rate of planthoppers, demonstrating that the NSvc2-N expressed in the transgenic plants... S The
protein can competitively bind to the
viral infection site located in the
midgut of the
planthopper, thereby blocking the
planthopper from transmitting the virus. This method fills the gap in technology that uses the
plant itself to block the planthopper from transmitting the virus. Compared with traditional "
insect control and
disease prevention" methods, this method is simple, economical, green, and efficient, and can be applied to antiviral breeding,
disease control research, and research on the mechanism of
insect virus transmission.