HPLC finger-print detection method of Heracleum souliei

A Kangding independent living and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as differences in drug efficacy, and achieve high precision, good separation, and good repeatability

Inactive Publication Date: 2015-12-16
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that although the properties of the two are similar, there are obvious differences in their efficacy. If the two are mixed in clinical practice, there will be great risks.

Method used

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  • HPLC finger-print detection method of Heracleum souliei
  • HPLC finger-print detection method of Heracleum souliei
  • HPLC finger-print detection method of Heracleum souliei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 The HPLC fingerprint spectrum detection method of Kangding Duhuo of the present invention

[0049] (1) Preparation of the reference solution: Accurately weigh 0.5g of Kangding Duhuo powder, place it in a 50mL conical flask, add 30mL of methanol, weigh after cold soaking for 60min, ultrasonic (power 200W, frequency 40KHZ) for 30min, take out, Let cool, add weight, and take the supernatant to pass through a 0.45 μm microporous membrane as a reference solution;

[0050] (2) Preparation of reference solution: accurately weigh appropriate amounts of psoralen reference substance and bergamot lactone reference substance, mix them, and use a 10mL volumetric flask to constant volume to prepare psoralen and bergamot lactone concentrations of 0.086mg / mL and 0.358mg / mL methanol solutions, as the reference solution;

[0051] (3) Establishment of contrast fingerprints:

[0052] The reference substance solution and the reference substance solution prepared from 1-10 bat...

Embodiment 2

[0059] Example 2 Conditional screening and methodological investigation of the detection method of the present invention

[0060] The test article under the present embodiment is selected from the medicinal materials of Kangding Independence in Table 1.

[0061] 1 Chromatographic conditions

[0062] Using DiamonilC 18 Chromatographic column (250×4.6mm, 5μm), the mobile phase A is water, the mobile phase B is methanol, the column temperature is 30°C, the flow rate is 1mL / min, the detection wavelength is 309nm, the inspection time is 50min, and the injection volume is 20μL. Gradient elution: 45% B for 5 minutes, 45%-60% B for 5 minutes, 60%-73% B for 25 minutes, 73%-80% B for 5 minutes, and 80% B for 10 minutes, namely:

[0063]

[0064] 1.1 Selection of detection wavelength

[0065] Adopt diode array detector, carry out full-wavelength scanning to need testing solution, compare HPLC-3D figure under each wavelength, the result is as follows Figure 4 shown.

[0066] The ...

Embodiment 3

[0124] Embodiment 3 The present invention measures the method for psoralen and bergamot lactone in Kangding Duhuo

[0125] 1 Linear relationship investigation

[0126] Accurately weigh the appropriate amount of psoralen reference substance and bergamot lactone reference substance, mix them, and use a 10mL volumetric flask to make up the concentration of psoralen and bergamot lactone to be 0.086mg / mL and 0.358mg / mL respectively methanol solution to obtain a mixed reference solution of bergamotide and psoralen.

[0127] Precisely draw 0.4, 0.8, 1, 4, 8, 12, 20 μL of the mixed reference solution of bergamot lactone and psoralen, and measure according to the chromatographic conditions in Example 1, with the injection volume as the abscissa and the peak area as the ordinate Coordinates, the regression equations are Y=3540.5X+339.93 (R 2 =0.9982), Y=2421.0X-23.790 (R 2 =0.9982), the linear ranges were 0.1432-7.16mg and 0.0344-1.72mg, respectively. The result is as Figure 10 an...

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Abstract

The invention discloses a HPLC finger-print detection method of Heracleum souliei. The finger-print has multiple peaks, good resolution, good repeatability and high precision, and thereby provides an effective guarantee for comprehensive monitor of Heracleum souliei quality. By means of an extraction method and a chromatogram condition of the finger-print detection method, contents of two active components psoralen and bergapten in Heracleum souliei can also be detected, so that quality of Heracleum souliei can be controlled from more aspects according to the invention.

Description

technical field [0001] The invention relates to the technical field of drug quality control methods, in particular to an HPLC fingerprint detection method of Kangding Duhuo. Background technique [0002] Kangding Duhuo is the dry root of Kangding Duhuo plant Heracleum soulieide Boiss. of Umbelliferae. Because Kangding Duhuo has similar properties to Bailiang Duhuo, it is generally considered to be a counterfeit of Tibetan medicine Bailiang Duhuo (Zhuga). [0003] However, the inventor found through previous studies that Kangding Duhuo showed a significant curative effect in terms of analgesia, but Bailiang Duhuo did not show a curative effect; Living alone is better. It can be seen that although the properties of the two are similar, there are obvious differences in their efficacy. If the two are mixed in clinical practice, great risks will arise. [0004] Therefore, in order to prevent the mixed use of drugs, there is an urgent need for a method to monitor the quality of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 兰志琼邓晶晶卢先明蒋桂华马逾英高必兴连艳陈虹宇尹显梅郑立艾青青陈玲
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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