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Constructing method of electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and standard fingerprint

A technology of electrospray mass spectrometry and fingerprints, which is applied in the field of standard fingerprints, can solve the problems of long chromatographic separation time, unable to meet fast and high throughput, and achieve good reproducibility, simple method, accuracy, authenticity and quality Effect

Inactive Publication Date: 2013-02-06
CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional fingerprinting method is chromatography, which cannot meet the requirements of fast and high throughput due to the long chromatographic separation time

Method used

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  • Constructing method of electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and standard fingerprint
  • Constructing method of electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and standard fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Establishment of Electrospray Mass Spectrometry Fingerprint of Guangdong Herbal Tea Granules

[0023] 1 Instruments and reagents

[0024] 1.1 Instruments Agilent 6210 Flight Reagent Mass Spectrometer, MassHunter Mass Spectrometry Workstation.

[0025] 1.2 Reagents Malic acid, quinic acid, gluconic acid, chlorogenic acid and isochlorogenic acid reference substances; acetonitrile (chromatographically pure), methanol (chromatographically pure), twice redistilled water.

[0026] 2. Experimental part

[0027] 2.1 Mass spectrometry conditions: direct sample injection by portable peristaltic pump, flow rate 50 μL / min; electrospray ion source; electrospray voltage 5000V; auxiliary spray nitrogen pressure 15psi; dry nitrogen temperature 350°C; dry nitrogen flow rate 5L min -1 ; Fragmentor voltage 100V; cone voltage 65V; octopole radio frequency voltage 250V; full scan mode to record mass spectrum profile, scan mass range m / z 100 ~ 1000.

[0028] 2.2 Preparation of ...

Embodiment 2

[0038] Example 2.6 standard fingerprints of Guangdong herbal tea granules from different sources

[0039]Take 15 batches of Wanglaoji sugar-free Guangdong herbal tea granules, 12 batches of Wanglaoji sugar-containing Guangdong herbal tea granules, 8 batches of Xiangxue sugar-containing Guangdong herbal tea granules, 6 batches of Guanhe sugar-containing Guangdong herbal tea granules, and 2 batches of Longma sugar-containing Guangdong herbal tea granules Two batches of Cihesu Yongkang sugar-containing Guangdong herbal tea granules were tested according to the conditions in Example 1. Each sample was measured three times, and the average value of the spectra obtained from the three measurements was exported as a .csv file in ASCII code format, and then through the software Convert it into a .txt file, import the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004A Edition" recommended by the Chinese Pharmacopoeia Commission, and use the mean method to c...

Embodiment 3

[0040] Example 3. Distinguishing the source of Guangdong herbal tea granules by performing principal component analysis on fingerprints

[0041] Take 15 batches of Wanglaoji sugar-free Guangdong herbal tea granules, 12 batches of Wanglaoji sugar-containing Guangdong herbal tea granules, 8 batches of Xiangxue sugar-containing Guangdong herbal tea granules, 6 batches of Guanhe sugar-containing Guangdong herbal tea granules, and 2 batches of Longma sugar-containing Guangdong herbal tea granules 2 batches of Cihesu Yongkang sugar-containing Guangdong herbal tea granules were tested according to the conditions of Example 1. Each sample was repeatedly measured three times, and the average value of the spectrograms obtained by the three measurements was exported as a .csv file in ASCII code format, and then statistically The principal component analysis (PCA) was carried out by the computer science software, and the chemical identification ions for source differentiation can be well r...

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Abstract

The invention provides a constructing method of an electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and a standard fingerprint, and relates to a quality control method of a traditional Chinese medicine preparation, in particular to a method for establishing the electrospray ionization mass spectrometry fingerprint to achieve quality evaluation and source distinguishment of the Guangdong herbal tea granules. The method comprises the following steps: (1) preparation of a test solution; (2) mass spectrometry condition: electrospray ionization mass spectrometry is directly used for detection, mass spectrometry contour map is recorded in a full-scanning mode, and scanning quality range m / z is 100-1000; (3) detection: the fingerprint is directly obtained by the electrospray ionization mass spectrometry; (4) standard fingerprint: the fingerprint of a test product uses the fingerprint according to the Similarity Evaluation System for Chromatographic Fingerprint of TCM(2004 A edition); and the standard fingerprint is generated through calculation using a mean value method; and (5) main ingredient analysis: main ingredients of the fingerprint of the test product are analyzed by using statistics software, so as to evaluate the quality and distinguish sources. The constructing method of an electrospray ionization mass spectrometry fingerprint of the Guangdong herbal tea granules, provided by the invention, is beneficial for monitoring product quality and has the advantages as follows: the quality of the Guangdong herbal tea granules is rapidly, efficiently and reliably represented; simple and convenient method, stability, high sensitivity and good repetitiveness are achieved; requirements on high throughput analysis are satisfied; and the quality of a product can be rapidly and accurately identified.

Description

technical field [0001] The invention relates to a method for quality evaluation and source differentiation of Guangdong herbal tea granules, in particular to a method for constructing a direct electrospray mass spectrometry fingerprint and a standard fingerprint of Guangdong herbal tea granules. Background technique [0002] Guangdong herbal tea is a kind of plant developed and summarized by the people of Lingnan according to the local climate and water and soil characteristics, guided by the theory of traditional Chinese medicine health preservation and based on Chinese herbal medicine in the process of long-term disease prevention and health care. drinks. Guangdong herbal tea granule is one of the famous Guangdong herbal teas. It is composed of 10 medicinal materials, such as gang plum, mountain sesame, five-finger mandarin orange, light bamboo leaf, wood butterfly, cloth dregs leaf, fire charcoal mother, golden sand rattan, broad money grass and golden cherry root. It has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/34
Inventor 杨运云邓洁薇张中强宋化灿范春林
Owner CHINA NAT ANALYTICAL CENT GUANGZHOU
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