Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules
A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine
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Embodiment 1
[0070] Embodiment 1 A method for detecting fingerprints of kidney-tonifying and pregnant-supporting granules, comprising the following steps:
[0071] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:
[0072] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, seal tightly, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with 70% volume concentration of methanol water, shake well, filter with 0.45μm microporous membrane, and take the subsequent filtrate to obtain
[0073] Step 2, preparation of mixed reference solution:
[0074] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, astragalin, rosmarinic ...
Embodiment 2
[0079] A method for detecting fingerprints of kidney-tonifying and pregnant-supporting granules, comprising the following steps:
[0080] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:
[0081] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, and accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, close the plug, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with methanol water with a volume concentration of 70%, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate to obtain
[0082] Step 2, preparation of mixed reference solution:
[0083] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, astragalin, rosmarini...
Embodiment 3
[0089] Embodiment 3 A detection method of the fingerprint of kidney-tonifying and pregnant-supporting granules, which comprises the following steps:
[0090] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:
[0091] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, and accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, close the plug, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with methanol water with a volume concentration of 70%, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate to obtain
[0092] Step 2, preparation of mixed reference solution:
[0093] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, a...
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