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Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules

A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine

Active Publication Date: 2020-05-19
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no preparation approval number at present. Therefore, it is necessary to conduct pharmaceutical research on preparations and new drug research in medical institutions, including preparation technology, quality standards, and stability tests.

Method used

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  • Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules
  • Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules
  • Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 A method for detecting fingerprints of kidney-tonifying and pregnant-supporting granules, comprising the following steps:

[0071] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:

[0072] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, seal tightly, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with 70% volume concentration of methanol water, shake well, filter with 0.45μm microporous membrane, and take the subsequent filtrate to obtain

[0073] Step 2, preparation of mixed reference solution:

[0074] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, astragalin, rosmarinic ...

Embodiment 2

[0079] A method for detecting fingerprints of kidney-tonifying and pregnant-supporting granules, comprising the following steps:

[0080] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:

[0081] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, and accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, close the plug, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with methanol water with a volume concentration of 70%, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate to obtain

[0082] Step 2, preparation of mixed reference solution:

[0083] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, astragalin, rosmarini...

Embodiment 3

[0089] Embodiment 3 A detection method of the fingerprint of kidney-tonifying and pregnant-supporting granules, which comprises the following steps:

[0090] Step 1, the preparation of the test product solution of Bushen Zhuyun Granules:

[0091] Accurately weigh the above-mentioned 15 batches of kidney-reinforcing and pregnancy-supporting granules, grind them finely, and accurately weigh 0.8g, place them in a stoppered Erlenmeyer flask, accurately add 10mL of methanol water with a volume concentration of 70%, close the plug, weigh, 200W, Ultrasonic treatment at 40kHz for 30min, put it at room temperature, weigh again, make up the lost amount with methanol water with a volume concentration of 70%, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate to obtain

[0092] Step 2, preparation of mixed reference solution:

[0093] Accurately weighed gallic acid, 5-hydroxymethylfurfural, morroniside, loganin, daniflorin, paeoniflorin, hyperoside, a...

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Abstract

The invention discloses a detection method of a fingerprint spectrum of kidney-tonifying pregnancy-assisting granules. The detection method comprises the following steps: step 1, preparing a kidney-tonifying pregnancy-assisting granule test solution; step 2, preparing a mixed reference substance solution; step 3, precisely sucking the mixed reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and recording chromatograms; step 4, exporting a fingerprint spectrum instrument of the kidney-tonifying pregnancy-assisting granules, importing the fingerprint spectrum instrument into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of kidney-tonifying pregnancy-assisting granules as common peaks; generating a reference fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules by usingan average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing the fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules with the spectrum of a mixed standard substance, and identifying the peak of main components. The fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules provided bythe invention can comprehensively and objectively represent the quality of the kidney-tonifying pregnancy-assisting granules. And the detection method has the advantages of simplicity, convenience, stability, high precision, good reproducibility and the like.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of the fingerprint of kidney-tonifying and pregnancy-supporting granules. Background technique [0002] The prescription of Bushen Zhuyun Granules is the empirical prescription of Professor Xia Guicheng, a master of traditional Chinese medicine in Jiangsu Provincial Hospital of Traditional Chinese Medicine. It has the effect of making the "Yang grow to be heavy", relaxing the heart and liver qi, and stabilizing the heart, liver and soul. It is clinically used to treat infertility with kidney deficiency and partial yang, and liver depression type luteal insufficiency. It has been used for many years and has long-term clinical experience. After multiple pharmacological tests to reveal the curative effect. However, there is no preparation approval number yet. Therefore, it is very necessary to conduct pharmaceutical research on preparations an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809Y02A50/30
Inventor 刘志辉周惠芳范智毅周洪亮姜誉弘
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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