Method for detecting fingerprint spectrum of Guigui zhugan decoction
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A fingerprint and detection method technology, which is applied in the detection field of traditional Chinese medicine prescriptions, can solve the problems of less research on detection methods, and achieve the effects of good reproducibility, good stability, and simple methods
Active Publication Date: 2019-05-03
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +2
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[0004] At present, there are few studies on the fingerprint detection methods of Lingguizhugan Decoction
Method used
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Embodiment 1
[0043] 1, a kind of detection method of Linggui Zhugan Decoction fingerprint, is characterized in that, comprises the following steps:
[0044] Step 1, the preparation of Linggui Zhugan Decoction test solution:
[0045] Precisely weigh 10 batches of Poria cocos, Guizhi, Atractylodes macrocephala and licorice, including 40g of Poria cocos, 30g of cinnamon twig, 20g of Atractylodes macrocephala and 20g of licorice. Add 1L of water for ultrasonic extraction, collect the medicinal solution by filtration, evaporate the medicinal solution to dryness, add 4 times the amount of 95% ethanol and heat under reflux for extraction for 3 hours, filter the supernatant, evaporate to dryness in a water bath, add 20mL methanol solution to dissolve, pass through 0.45μm Pore filter membrane, get 10 batches of need testing solution;
[0046] Step 2, preparation of reference substance solution:
[0047] , the preparation method of the reference substance solution in step 2 is as follows: take acc...
Embodiment 2
[0064] Embodiment 2 methodological investigation
[0065] 1. Precision experiment
[0066] Precisely draw the reference substance solution under embodiment 1, and continuously inject samples 6 times by the chromatographic conditions under embodiment 1, the RSD of its main common peak retention time is all less than 0.95%, and the relative peak area RSD is all less than 2.44%, showing the precision of the instrument The degree is good. The specific experimental results are shown in Table 6 and Table 7.
[0067] Table 6 relative retention time
[0068] serial number
peak 1
peak 2
peak 3
peak 4
peak 5
Peak 6
1
30.228
31.127
33.156
38.209
46.377
61.193
2
30.507
31.096
33.253
38.185
46.182
61.137
3
30.103
31.224
33.087
38.311
46.134
61.353
4
30.862
31.034
33.076
38.409
46.138
61.527
5
30.130
31.525
33.174
38.244
46.271
61.556
...
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Abstract
The invention discloses a method for detecting a fingerprint spectrum of Guigui zhugan decoction. The fingerprint spectrum is established by the following steps: S1, preparing a Guigui zhugan decoction test sample solution; S2, preparing a reference solution; S3, absorbing the test sample solution and the reference solution precisely and respectively and injecting the solutions into a liquid chromatograph, and recording a chromatogram map; and S4, outputting a Guigui zhugan decoction fingerprint spectrum obtained in the S3 into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks exiting in all chromatogram maps of Guigui zhugan decoction with different batches as common peaks and generating a contrast fingerprint spectrumof the Guigui zhugan decoction based on an average value calculation method, and calculating relative retention time and relative peak areas of all common peaks. According to the invention, the detecting method having advantages of high stability and precision, and high reproducibility and the like is capable of analyzing the effective components in the Guigui zhugan decoction accurately and hasthe important significance in evaluating the quality of the Guigui zhugan decoction comprehensively and objectively.
Description
technical field [0001] The invention relates to a detection method of a traditional Chinese medicine prescription, in particular to a detection method of a fingerprint of Linggui Zhugan Decoction. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine, and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and traditional Chinese medicine preparations. Traditional Chinese medicine and its preparations are multi-component complex sys...
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