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Fingerprint detection method for compound wintercreeper preparation

A compound Fufangteng and fingerprint technology, which can be used in anti-toxic agents, pill delivery, measuring devices, etc., can solve the problems of high repeatability and low detection sensitivity of saponins

Inactive Publication Date: 2011-02-09
广西中医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The solution of the present invention is to provide a HPLC-ELSD fingerprint of the main saponin components in the compound fufangteng preparation that has a better detection effect on the saponins components Atlas Inspection method, this method not only has qualitative effect (by fingerprint peak position), but also has certain quantitative effect (by correlation peak area), and can overcome the shortcoming that HPLC-UV is not high to the detection sensitivity of saponins, according to this method determined fingerprint Atlas With high repeatability, it can be used not only as an inspection and identification method for the main saponin components in the compound Fufangteng preparation, but also for the content determination of astragaloside IV and ginsenoside Rb1 in the preparation

Method used

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  • Fingerprint detection method for compound wintercreeper preparation
  • Fingerprint detection method for compound wintercreeper preparation
  • Fingerprint detection method for compound wintercreeper preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Compound Fufangteng Mixture Control Fingerprint Atlas Determination of

[0088] 1 Instruments and reagents

[0089] 1.1 Instrument: American Agilent 1100 high performance liquid chromatography, including online vacuum degasser (G-1322A), high-pressure quaternary gradient pump (G-1311A), standard autosampler (G-1313A), intelligent column temperature Box (G-1316A), Agilent chromatography workstation data processing system. PL-ELS2100 evaporative light scattering detector (PL company), Shim-Pack CLC-ODS (6.0mmID × 150mm) chromatographic column (Shimadzu Corporation of Japan), constant temperature water bath (Jiangsu Medical Instrument Factory), BP211D electronic balance (Germany Sartorius), Simplicity 185 ultrapure water processor (Millipore, USA), LG16-W centrifugal sedimentation machine (Beijing Medical Centrifuge Factory), 0.45 μm microporous membrane (Millipore, USA).

[0090] 1.2 Reagent

[0091] Acetonitrile is chromatographically pure (Fisher Scientifi...

Embodiment 2

[0099] Preparation of Example 2 Compound Fufangteng Capsules and Its Control Fingerprint Atlas Determination of

[0100] 1 Preparation of Compound Fufangteng Capsules

[0101] Weigh Fufangteng and Astragalus medicinal materials qualified according to the Pharmacopoeia according to the proportion of the prescription, add water to decoct twice, each time for 2 hours, combine the filtrate, filter, and concentrate the filtrate to an extract with a relative density of 1.12-1.16 (60°C) , let cool, add ethanol to make the alcohol content 63%, stir, let stand for 48 hours, filter, and the filtrate is set aside. Take the ginseng medicinal material, add 65% ethanol and heat and reflux extract three times, each time for 2 hours, combine the extracts, filter, combine the filtrate with the above filtrate, recover the ethanol and concentrate to the extract with a relative density of 1.26 (80°C), let it cool , add starch dried at 100°C for 24 hours, stir evenly, granulate, dry, pulverize, ...

Embodiment 3

[0111] Example 3 Compound Fufangteng Tablet Control Fingerprint Atlas the establishment of

[0112] 1 Preparation of Compound Fufangteng Tablets:

[0113] Weigh the qualified red ginseng medicinal materials according to the prescription ratio, heat and reflux with 65% ethanol for three times, each time for 2 hours, combine the extracts, filter, and the filtrate is ready for use; add water to the dregs and decoct three times, each time for 1.5 hours, Combine the decoctions, filter, concentrate the filtrate to a relative density of about 1.06 (60°C), let it cool, refrigerate for more than 48 hours, filter, and use the filtrate for later use; add Fufangteng and Astragalus to decoct twice, each time for 2 hours, combine Decoction, filter, concentrate the filtrate to a relative density of about 1.14 (60°C), let it cool, add 2 times the amount of ethanol, stir well, let it stand for more than 48 hours, filter, and the filtrate with the ethanol extract of red ginseng and water The ...

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Abstract

The invention provides a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) fingerprint detection method for a main saponin component of a compound wintercreeper preparation (including mixture, capsules and tablets). The fingerprint detection method of the invention comprises the steps of: preparing test solution and measuring the fingerprint under a certain chromatographic condition. Scientific tests prove that the fingerprint measured by the method has high repeatability, so that the method can be used as a method for checking and identifying the main saponin component of the compound wintercreeper preparation and can also be used for measuring astragaloside content and ginsenoside Rb1 content of the preparation.

Description

technical field [0001] The invention relates to the fingerprint of a traditional Chinese medicine compound fufangteng preparation Atlas The detection method belongs to the field of analysis and detection of compound traditional Chinese medicine preparations. Background technique [0002] The compound Fufangteng preparation is a compound preparation made from Fufangteng, astragalus, and red ginseng (or ginseng) through proper processing and extraction. Fatigue, improve the body's immunity, etc., especially suitable for patients with qi and blood deficiency, lack of energy, insomnia, dreaminess, neurasthenia, etc. The quality control method adopted at present is to carry out according to the quality standard of compound Fufangteng mixture recorded in "Chinese Pharmacopoeia" 2005 edition one. In addition to the TLC identification in the current quality standard, there is only one content determination item of thin-layer chromatography scanning, which is used to determine the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/48A61K9/00A61P25/00A61P37/04A61P39/00A61P25/20A61K36/481A61K9/20G01N30/02G01N30/36A61P7/06
Inventor 覃洁萍邓家刚冯军李芸李耀华冯旭
Owner 广西中医学院
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