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Construction method and quality detection method of UPLC feature map of Hangzhou chrysanthemum medicinal material

A quality inspection method and characteristic map technology, which is applied in the construction method of UPLC characteristic map and the field of quality inspection, can solve the problems of inability to reflect the quality of chrysanthemums, confusion, and inability to reflect the quality of chrysanthemums, etc., to achieve comprehensive monitoring of product quality and rich information , the effect of reducing the possibility of human handling

Active Publication Date: 2019-02-22
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Aware of this problem, some scholars have compared and evaluated the internal components of different varieties of chrysanthemums from the perspective of multi-index control, but the multi-component analysis brings comprehensive information and at the same time causes confusion.
This status quo cannot reflect the quality of chrysanthemums well, let alone the quality of Hangchrysanthemums.

Method used

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  • Construction method and quality detection method of UPLC feature map of Hangzhou chrysanthemum medicinal material
  • Construction method and quality detection method of UPLC feature map of Hangzhou chrysanthemum medicinal material
  • Construction method and quality detection method of UPLC feature map of Hangzhou chrysanthemum medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Construction of the characteristic map of Chrysanthemum chrysanthemum

[0050] 1. Instruments, reagents, reagents and evaluation software

[0051] 1.1 Instrument

[0052] Thermo ultra-high performance liquid chromatography (Vanquish (DAD), Thermo Fisher); Waters HSS-T 3 Column (100×2.1mm, 1.8μm); Agilent ultra-high performance liquid chromatography (1290Infinity, Agilent Corporation); Mettler XP26 millionth balance (Swiss Mettler Toledo company); KQ5500DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); constant temperature water bath (Shanghai Yiheng Technology Co., Ltd., model: HWS28); Milli-Q ultrapure water purification system (Millipore, USA).

[0053] 1.2 Reagents

[0054] Ethanol (Tianjin Fuyu Fine Chemical Co., Ltd., analytically pure); methanol (Tianjin Fuyu Fine Chemical Co., Ltd., analytically pure); phosphoric acid (Tianjin Kemiou Chemical Reagent Co., Ltd., chromatographically pure); acetonitrile (Merck Co., Ltd., chromatograph...

Embodiment 2

[0149] Embodiment 2: A kind of quality detection method of Hangchrysanthemum medicinal material and decoction pieces

[0150] Preparation of the test solution: take 0.4 g of the powder of the chrysanthemum to be tested (S22-S27) (through the No. 1 sieve), accurately weigh it, put it in a stoppered conical flask, and accurately add 70% (v / v) methanol 25 mL of the solution was weighed, heated to reflux for 40 minutes, taken out, allowed to cool, and weighed again, supplemented with 70% (v / v) methanol solution to reduce the lost weight, shake well, filter, and take the filtrate to obtain the final product.

[0151] Preparation of reference solution: same as under "2.2 Preparation of reference solution".

[0152] Chromatographic conditions and system adaptability test: same as under "2.3 Chromatographic conditions".

[0153] Determination method: same as under "2.4 Determination method".

[0154] Establishment of qualified indicators and formulating spectra and judgment method...

Embodiment 3

[0155] Embodiment 3: a kind of quality detection method of chrysanthemum medicinal material

[0156] Preparation of the test solution: Take an appropriate amount of this product powder (batch numbers: S28, S29, passed through a No. 1 sieve), grind it finely, take about 0.4g, accurately weigh it, put it in a stoppered Erlenmeyer flask, and add 70% methanol precisely 25mL, weighed, heated to reflux for 40min, taken out, allowed to cool, weighed again, made up for lost weight with 70% methanol, shaken well, filtered, and obtained the filtrate.

[0157] Preparation of reference solution: same as under "2.2 Preparation of reference solution".

[0158] Chromatographic conditions and system adaptability test: same as under "2.3 Chromatographic conditions".

[0159] Determination method: same as under "2.4 Determination method".

[0160] Judgment method of qualified indicators: tentatively determined that the qualified indicators of the chrysanthemum medicinal material are 10 char...

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Abstract

The invention relates to a construction method and a quality detection method of a UPLC feature map of a Hangzhou chrysanthemum medicinal material. The construction method comprises the following steps: sample solution preparation; taking the Hangzhou chrysanthemum medicinal material, crushing, adding a solvent for extraction and filtering, wherein a filtrate is taken as a sample solution; reference solution preparation: taking chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, galuteolin and linarin and adding a solvent for dissolution, wherein an obtained solution isa reference solution; ultra performance liquid chromatography detection: absorbing the sample solution and the reference solution, injecting into a liquid chromatograph and detecting to obtain the UPLC feature map of the Hangzhou chrysanthemum medicinal material. The UPLC feature map is rich in characteristic peak information, can fully reflect the chemical composition of the Hangzhou chrysanthemum medicinal material and can also effectively characterize the qualities of a Hangzhou chrysanthemum decoction piece and standard decoction and identify the product authenticity.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a construction method and a quality detection method of a UPLC characteristic map of a chrysanthemum medicinal material. Background technique [0002] Chrysanthemum is the dry flower head of Chrysanthemum morifolium Ramat. Chrysanthemum was first recorded in "Shen Nong's Materia Medica" in the Eastern Han Dynasty, and was listed as top grade. It is said: "long-term use can benefit blood qi, make light of body, resist aging, and prolong life". For anemopyretic cold, headache and dizziness, conjunctival congestion and swelling pain, blurred vision, sore carbuncle and swollen toxin. The 2015 edition of the Chinese Pharmacopoeia contains five main medicinal chrysanthemums: "Gongju", "Chuju", "Boju", "Hangju" and "Huaiju". After consulting a large number of literatures, it is found that the types and contents of the main chemical components of chrysanthemums are d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 魏梅陈菊英霍文杰程学仁丁青杨晓东刘晓霞陈向东
Owner GUANGDONG YIFANG PHARMA
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