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Five magic skin healthy ointment fingerprint detection method

A fingerprint and detection method technology, which is applied in the detection field of Chinese patent medicines, can solve the problems such as the lack of Wumiao Narcissus paste, and achieve the effect of simple method, avoiding one-sidedness and good stability

Inactive Publication Date: 2019-03-19
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few quality detection methods for Wumiao Narcissus ointment. The present invention adopts high performance liquid chromatography to establish the fingerprint detection method of Wumiao Narcissus ointment, accurately analyzes the active ingredients in Wumiao Narcissus ointment, and comprehensively and objectively It is of great significance to evaluate the quality of Wumiao Narcissus ointment

Method used

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  • Five magic skin healthy ointment fingerprint detection method
  • Five magic skin healthy ointment fingerprint detection method
  • Five magic skin healthy ointment fingerprint detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1, a kind of detection method of Wumiao Narcissus paste fingerprint, is characterized in that, comprises the following steps:

[0044] Step 1, preparation of Wumiao Narcissus Ointment test solution:

[0045] Take 10 batches of the above-mentioned Wumiao Narcissus paste samples, accurately weigh 5g of the paste, put it in a 100mL conical flask, add 25mL of methanol solution containing 1% hydrochloric acid, and perform ultrasonic treatment for 15 minutes to obtain the filtrate by suction filtration. Repeat the extraction with 25 mL of methanol solution containing 1% hydrochloric acid, collect the filtrate obtained twice, evaporate to dryness in a water bath, add methanol containing 1% hydrochloric acid to dissolve to 25 mL, and pass through a 0.45 μm microporous membrane to obtain the test solution.

[0046] Step 2, preparation of reference substance solution:

[0047] Get the accurately weighed berberine hydrochloride reference substance, place it in a volumetric flask,...

Embodiment 2

[0063] Embodiment 2 methodological investigation

[0064] 1. Precision experiment

[0065] Precisely draw the reference substance solution under embodiment 1, and continuously inject samples 6 times by the chromatographic conditions under embodiment 1, the RSD of its main common peak retention time is all less than 0.24%, and the relative peak area RSD is all less than 2.82%, showing the precision of the instrument The degree is good. The specific experimental results are shown in Table 5 and Table 6.

[0066] Table 5 relative retention time

[0067] serial number

peak 1

peak 2

peak 3

peak 4

1

31.318

33.287

38.377

50.834

2

31.251

33.203

38.38

50.833

3

31.216

33.162

38.396

50.85

4

31.171

33.109

38.382

50.831

5

31.163

33.101

38.536

50.814

6

31.129

33.062

38.368

50.829

average value

31.208

33.154

38.389

50.831

RSD

0.2...

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Abstract

The invention discloses a five magic skin healthy ointment fingerprint detection method which includes the steps: 1 preparing five magic skin healthy ointment test solution; 2 preparing reference solution; 3 precisely sucking quantitative test solution and reference solution, injecting the solution into a liquid chromatograph and recording a chromatogram; 4 leading out a five magic skin healthy ointment fingerprint instrument obtained in the step 3, leading the chromatogram into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks of chromatograms of five magic skin healthy ointments in different batches as common peaks, generating the reference fingerprint of the five magic skin healthy ointments by an average computing method, and calculating relative retention time and relative peak area of the common peaks. The researched five magic skin healthy ointment fingerprint detection method has the advantages that the method is simple, clear in fingerprint, high in repeatability and precision and the like. The quality of the five magic skin healthy ointments can be comprehensively and objectively represented.

Description

technical field [0001] The invention relates to a detection method of a Chinese patent medicine, in particular to a detection method of the fingerprint of Wumiao Narcissus Ointment. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine, and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and traditional Chinese medicine preparations. Traditional Chinese medicine and its preparations are multi-component complex systems, so the ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蔡宝昌陈海超金俊杰李国维郑艳萍张金龙张卉
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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