HPLC fingerprint determination method of cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills

A technique for treating cough, Chuanbei loquat, and a determination method, which is applied in the field of medicine and achieves the effects of being easy to grasp, having good stability and reducing the possibility of human processing.

CN103487528AActive Publication Date: 2014-01-01津药达仁堂集团股份有限公司第六中药厂
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-01-01

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Abstract

An HPLC (high performance liquid chromatography) fingerprint determination method of cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills comprises the following steps: 1, cough relieving Bulbus fritillariae cirrhosae and loquat dripping pill tested substance solution preparation: preciously weighing the cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills, and carrying out ultrasonic extraction of the cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills by using 30-100% ethanol as an extraction solvent to prepare a tested substance solution; and 2, injecting the solution obtained in step 1 into a high performance liquid chromatograph, and determining to obtain a cough relieving Bulbus fritillariae cirrhosae and loquat dripping pill fingerprint. An HPLC method is adopted to establish the fingerprint according to the self characteristics of the cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills, and optimal chromatogram conditions are found, so the determination result is precious, and has a good reappearance and a good stability.
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Description

technical field

[0001] The invention belongs to the technical field of medicines, and in particular relates to an HPLC fingerprint determination method for cough-treating Chuanbei Piqua dripping pills. Background technique

[0002] Cough Chuanbei Piqua Dropping Pills are composed of loquat leaves, flat fritillaria, bellflower, water pinellia, and menthol, and are refined by modern preparation technology. Cough caused by heat stagnation in the lung, symptoms include cough, expectoration, dry throat, sore throat, fever, general malaise; cold and bronchitis with the above symptoms, it is a traditional Chinese medicine that is protected by the state.

[0003] The quality of traditional Chinese medicines is, in the final analysis, the differences in the chemical components and their contents. This difference can be reflected by the chromatographic fingerprint of traditional Chinese medicine. As an effective control method for holistic evaluation of the quality of traditional Ch...

Examples

Embodiment 1

[0031] Embodiment 1: The method for determining the HPLC fingerprint of Chuanbei Piqua Dropping Pills

[0032] 1.1 Instruments and reagents

[0033] Instrument: Waters ACQUITY TM UPLC ultra-high performance liquid chromatography, Masslynx TM 4.0 workstation;

[0034] Chromatographic column: ACQUITY UPLC HSS T3, 1.8um, 2.1×100mm, Waters

[0035] Reagents: Methanol, acetonitrile, and formic acid are chromatographically pure (Fisher), water is ultrapure water, and other reagents are analytically pure.

[0036] 1.2 Preparation of reference solution

[0037] Chlorogenic acid (self-made, structure confirmed by MS, UV, FIR, and NMR) has a retention time of 7.99 minutes, which has a strong response under this condition, and can basically achieve baseline separation from the impurities before and after. Therefore, it is designated as the reference substance. Take an appropriate amount of chlorogenic acid, weigh it accurately, add methanol to prepare a solution containing 0.1 mg of ...