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Determination method for radix curcumae fingerprint and standard fingerprint thereof

A fingerprint and detection method technology, applied in the field of traditional Chinese medicine fingerprint analysis, can solve problems such as the inability to accurately express the volatile components of turmeric, extend the research on special requirements of varieties, etc., and achieve the effect of improving the quality control technology of medicinal materials

Active Publication Date: 2018-10-23
WUXI JIMIN KEXIN SHANHE PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The methods provided in the current literature are all universal detection methods for medicinal materials, and have not been studied for the special requirements of extended varieties
[0006] And the determination of general volatile components all adopts gas chromatography, but sometimes the detection high temperature of the gas phase (high temperature of the injection port) causes the degradation of some components, such as furadienes and furadienones in turmeric, which cannot accurately express the turmeric volatile components; therefore, the patent uses two detection methods, gas chromatography and liquid chromatography, to study the fingerprints of turmeric

Method used

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  • Determination method for radix curcumae fingerprint and standard fingerprint thereof
  • Determination method for radix curcumae fingerprint and standard fingerprint thereof
  • Determination method for radix curcumae fingerprint and standard fingerprint thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Instruments and reagents

[0066] 1.1 Instrument

[0067] One hundred thousandth electronic balance (METTLER TOLEDO), Agilent 7890A gas chromatograph, Waters liquid chromatograph. DB‐624 column (30m*0.25mm*1.40μm), Phenomenex Gemini‐NX C18 column (4.6×150mm, 5μm)

[0068] 1.2 Reagents

[0069] β-Elemene Reference Substance, Curcumadione Reference Substance, n-Hexane, Ethanol, Methanol

[0070] 2. Preparation of the test article

[0071] Preparation of gas phase test solution: take 1.5g of turmeric medicinal material, put it in a 1000ml flask, add 100ml of water and a few zeolite grains, measure according to the volatile oil determination method, add water from the upper end of the measuring device to fill the scale part and overflow into the flask. Then add 2ml of n-hexane, connected to the reflux device, heated to boiling and kept at a slight boil for 3 hours, allowed to cool, separated the n-hexane layer, put it in a 2ml measuring bottle, washed the volatile oi...

Embodiment 2

[0086] 1. Instruments and reagents

[0087] 1.1 Instrument

[0088] One hundred thousandth electronic balance (METTLER TOLEDO), Agilent 7890A gas chromatograph, Waters liquid chromatograph. TraceGOLD TG‐624 column (30m*0.25mm*1.40μm), Phenomenex Gemini‐NX C18 column (4.6×150mm, 5μm)

[0089] 1.2 Reagents

[0090] β-Elemene Reference Substance, Curcumadione Reference Substance, n-Hexane, Ethanol, Methanol

[0091] 2. Preparation of the test article

[0092] Preparation of gas phase test solution: take 1.5g of turmeric medicinal material, put it in a 1000ml flask, add 100ml of water and a few zeolite grains, measure according to the volatile oil determination method, add water from the upper end of the measuring device to fill the scale part and overflow into the flask. Then add 2ml of n-hexane, connected to the reflux device, heated to boiling and kept at a slight boil for 3 hours, allowed to cool, separated the n-hexane layer, put it in a 2ml measuring bottle, washed the v...

Embodiment 3

[0108] 1. Instruments and reagents

[0109] 1.1 Instrument

[0110] One hundred thousandth electronic balance (METTLER TOLEDO), Agilent 7890A gas chromatograph, Waters liquid chromatograph. OV‐1301 column (30m*0.25mm*1.40μm), Agilent Eclipse XDB‐C18 column (4.6×150mm, 5μm)

[0111] 1.2 Reagents

[0112]β-Elemene Reference Substance, Curcumadione Reference Substance, n-Hexane, Ethanol, Methanol

[0113] 2. Preparation of the test article

[0114] Preparation of gas phase test solution: take 1.5g of turmeric medicinal material, put it in a 1000ml flask, add 100ml of water and a few zeolite grains, measure according to the volatile oil determination method, add water from the upper end of the measuring device to fill the scale part and overflow into the flask. Then add 2ml of n-hexane, connected to the reflux device, heated to boiling and kept at a slight boil for 3 hours, allowed to cool, separated the n-hexane layer, put it in a 2ml measuring bottle, washed the volatile oil...

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Abstract

The invention relates to a determination method for radix curcumae fingerprint and a standard fingerprint thereof. The determination method comprises the following steps of preparing of a test samplesolution, preparing of a reference sample solution, determining by gas chromatography, and determining by liquid chromatography, so as to obtain a chromatogram map; contrasting the obtained standard radix curcumae gas fingerprint and the liquid fingerprint, wherein the qualified product is obtained when the obtained standard radix curcumae gas fingerprint is consistent with the liquid fingerprint.The determination method has the advantages that the determination method is simple, rapid, accurate, stable and reliable, and is used for controlling the quality of the radix curcumae; the quality of the radix curcumae can be more comprehensively, objectively and scientifically evaluated; the validity of the preparation is guaranteed.

Description

technical field [0001] The invention relates to a method for analyzing fingerprints of traditional Chinese medicines, in particular to a method for measuring fingerprints of turmeric, and the obtained gas-phase fingerprints and liquid-phase fingerprints of turmeric. technical background [0002] Turmeric has the effects of promoting blood circulation and relieving pain, promoting qi and relieving stagnation, clearing heart and cooling blood, promoting gallbladder and reducing jaundice. Blood-heat spits nosebleed, jaundice and red urine. It is used in Angong Niuhuang Pills, Shixiang Huisheng Pills, Niuhuang Qinggong Pills, Xingnaojing Injection, etc. [0003] There are three kinds of turmeric bases stipulated in the Chinese Pharmacopoeia, which are the dry root tubers of Curcuma wenyujinY.H.Chenet C.Ling, which is called Curcuma wenyujinY.H.Chenet C.Ling, and the main place of origin is Wenzhou, Zhejiang; the dry tuber roots of Curcuma longaL. Commonly known as yellow silk ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘薇薇甘国峰於江华闵江刘筱滢李婷
Owner WUXI JIMIN KEXIN SHANHE PHARMA
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