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Detection method and construction method of amino acid fingerprint of Xiaoer Qixing tea preparation

A technology for children's seven-star tea and fingerprints, which is applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of accurate authenticity, simple method and good reproducibility.

Active Publication Date: 2015-09-09
GUANGZHOU WANGLAOJI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above methods are all for the identification or content determination of individual components in the Xiaoer Qixing tea preparation, and the quality control methods for the Xiaoer Qixing tea preparation need to be further improved

Method used

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  • Detection method and construction method of amino acid fingerprint of Xiaoer Qixing tea preparation
  • Detection method and construction method of amino acid fingerprint of Xiaoer Qixing tea preparation
  • Detection method and construction method of amino acid fingerprint of Xiaoer Qixing tea preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Construction of fingerprints of amino acid components in Xiaoer Qixing tea preparation

[0042] 1 Instruments and equipment

[0043] Shimadzu LC-20AD high performance liquid chromatography (Shimadzu Corporation);

[0044] Sartorius CP225D analytical balance (d=0.01mg) (Germany Sartorius company);

[0045]Ratavapor R-Ⅱ rotary evaporator (Buchi, Switzerland);

[0046] SHZ-D(Ⅲ) Circulating water positive air pump (Gongyi Yuhua Instrument Co., Ltd.);

[0047] RTHW electric heating mantle (Gongyi City Yuhua Instrument Co., Ltd.);

[0048] SK-1 fast mixer (Jiangsu Jintan Jiamei Instrument Factory);

[0049] 2 Materials and reagents

[0050] Pediatric Qixing tea granule sample (provided by Guangzhou Wanglaoji Pharmaceutical Co., Ltd.);

[0051] 19 amino acid reference substances: glycine, aspartic acid, leucine, isoleucine, lysine, histidine, serine, proline, methionine, threonine, phenylalanine, alanine , valine, cysteine, arginine, tryptophan, glutamic acid, cystine, ...

Embodiment 2

[0096] Detection of Xiaoer Qixing tea preparations by using fingerprints of amino acid components in Xiaoer Qixing tea preparations

[0097] 1 Instruments and equipment

[0098] With embodiment 1.

[0099] 2 Materials and reagents

[0100] Another 10 batches of Xiaoer Qixing tea granule samples (provided by Guangzhou Wanglaoji Pharmaceutical Co., Ltd.), and other materials and reagents are the same as in Example 1.

[0101] 3 methods

[0102] 3.1 Preparation of derivatization reagents

[0103] With embodiment 1.

[0104] 3.2 Preparation of reference solution

[0105] With embodiment 1.

[0106] 3.3 Pretreatment of XZ-2 Cationic Resin

[0107] With embodiment 1.

[0108] 3.4 Preparation of the test solution

[0109] With embodiment 1.

[0110] 3.5 Chromatographic conditions

[0111] With embodiment 1.

[0112] Determination: Inject 10 μL of the reference solution mixed with 19 kinds of amino acids and the test solution respectively, inject it into a high-performance...

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Abstract

The invention discloses an infantile seven-element tea preparation amino acid fingerprint detection method and construction method. The detection method comprises the following steps: (1) preparation of a control solution; (2) preparation of a test sample solution; and (3) detection by a high performance liquid chromatograph. The method can effectively detect the quality of the infantile seven-element tea preparation, and is beneficial to monitoring the quality of the product; and the method is simple and stable, has the advantages of high precision and high reproducibility, and can quickly and accurately identify the authenticity and quality of the product.

Description

technical field [0001] The invention relates to a method for quality control of traditional Chinese medicine preparations, in particular to a detection method and a construction method for amino acid fingerprints of Xiaoer Qixing tea preparations. Background technique [0002] Children's Seven Star Tea preparation is made from Chinese medicinal materials coix seed, rice bud, hawthorn, light bamboo leaf, uncaria, cicada slough, and licorice, which are extracted with water and made according to conventional pharmaceutical processes. It has the effects of appetizing and stagnant, clearing heat and calming convulsions. It is used for children with stagnant heat, indigestion, lack of appetite, irritability and shock, restless night sleep, poor stool, and short urine. Pediatric Seven Star Tea preparations are included in the ninth volume of Chinese medicine prescription preparations of the Ministry of Health of the People's Republic of China, including Pediatric Seven Star Tea Gra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 方广宏郑荣波苏子仁黄晓丹彭绍忠
Owner GUANGZHOU WANGLAOJI PHARM CO LTD
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