Method for establishing Anweiyang capsule fingerprint spectrum

A technology for fingerprints and stomach ulcers, which is applied in the field of establishing the fingerprints of licorice extract capsules, can solve the problems of large influence, large operation error by gravimetric method, poor reproducibility and the like, and achieves simple method, good reproducibility, high precision effect

Active Publication Date: 2014-08-06
XINJIANG QUANAN PHARMA
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The current quality standard is to check the total content of dozens of total flavonoids in Anweiyang Capsules, which cannot reflect the characteristics of various flavonoids, and the quality has not been truly and effectively controlled. For example, the current quality standard number of Anweiyang Capsules is WS3 -002-(Z-002)-98-(Z), which stipulates that the method for determining the total flavonoid content is the gravimetric method. Due to the large operating error of the gravimetric method and the large influence of human factors, the reproducibility is poor

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  • Method for establishing Anweiyang capsule fingerprint spectrum
  • Method for establishing Anweiyang capsule fingerprint spectrum
  • Method for establishing Anweiyang capsule fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of the test solution

[0050] The present embodiment investigates the preparation method of need testing solution from the following aspects.

[0051] 1. Extraction solvent

[0052] Using water, 30% ethanol aqueous solution, 50% ethanol aqueous solution, 70% ethanol aqueous solution, and absolute ethanol as extraction solvents, the same batch of test samples was investigated by ultrasonic extraction method.

[0053] The results show (not shown in the figure), the extraction rate of water and 30% ethanol aqueous solution is too low, and the chromatographic peak components are almost not extracted after 40 minutes, the extraction effect of 50% ethanol aqueous solution and 70% ethanol aqueous solution is equivalent, and the extraction rate of absolute ethanol is low In 50% ethanol aqueous solution and 70% ethanol aqueous solution, therefore select 70% ethanol aqueous solution identical with the content determination method in the Pharmacopoeia as ...

Embodiment 2

[0060] Example 2 Chromatographic Conditions and System Suitability Test Investigation

[0061] In this example, the chromatographic conditions and system applicability of the fingerprint of Anweiyang Capsules were tested and investigated from the following aspects.

[0062] 1. Selection of fingerprint detection method

[0063] Literature research shows that the main components in licorice residue are flavonoids, which have good ultraviolet absorption, so HPLC-UV detection method is selected for fingerprint research.

[0064] 2. Selection of wavelength for fingerprint detection

[0065] Literature research shows that licorice dregs mainly contain flavonoids, most of which are chalcones, dihydroflavones, isoflavones, etc., and licorice grassland medicinal materials still contain glycyrrhizin components, and their ultraviolet absorption wavelength characteristics are different. The literature reports the use of wavelength gradients; a diode array detector is used to detect th...

Embodiment 3

[0089] Example 3 Detection of Licorice Flavonoids in Anweiyang Capsules

[0090] 1. Chromatographic column: the model is Agela Venusil MP 5μm C 18 -100? 4.6×250mm;

[0091] 2. Mobile phase: Acetonitrile is used as mobile phase A, 0.01% trifluoroacetic acid (TFA) solution is used as mobile phase B, and gradient elution is carried out according to the regulations in the table below;

[0092]

[0093] 3. Flow rate: 1.0mL / min;

[0094] 4. Detection wavelength: 270nm;

[0095] 5. The column temperature is 30°C;

[0096] 6. Preparation of reference substance solution: take an appropriate amount of licochalcone A reference substance, add 70% ethanol aqueous solution to make a solution containing 0.8mg per 1ml, and obtain it.

[0097] 7. Preparation of the test solution: Weigh 0.1g of the content of Anweiyang Capsules, put it in a 25ml measuring bottle, add 20ml of 70% ethanol aqueous solution, ultrasonically extract for 5 minutes, take it out, let it cool, and dilute to volum...

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Abstract

The invention provides a liquorice extract capsule and preferably a method for establishing an Anweiyang capsule fingerprint spectrum. According to the invention, a high-performance liquid chromatography method is adopted for detecting the content of flavonoids in a liquorice extract capsule, wherein the conditions of high-performance liquid chromatography are that a chromatographic column takes octadecyl silane bonded silica gel as a filling phase; acetonitrile and 0.01-0.1% trifluoroacetic acid in a volume ratio of 22:78-75:25 are taken as mobile phases for gradient elution; the flow velocity is 0.5-1.5 mL / min; the column temperature is 25-40 DEG C; and ultraviolet detection wavelength is 270nm. The establishing method provided by the invention is simple and convenient, stable, high in accuracy and good in reproducibility, can effectively represent the mass of the liquorice extract capsule, and is favorable for monitoring the quality of the product.

Description

technical field [0001] The invention belongs to the field of drug analysis and detection, and relates to a method for establishing fingerprints of licorice extract capsules. Background technique [0002] With the development of modern drug analysis technology, fingerprint technology is an internationally recognized quality control mode for controlling the quality of traditional Chinese medicine. Provide a full range of quality assurance. [0003] In recent years, foreign countries have also advocated fingerprinting for the quality evaluation of Chinese herbal medicine products. With the innovation and improvement of various analysis techniques and the continuous development of computer application technology, the understanding and research of natural medicines in various countries in the world are deepening. The fingerprint technology of traditional Chinese medicine has become a development trend at home and abroad. Its research methods and The technology has become more m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 王海峰屠鹏飞石子仪李海晶
Owner XINJIANG QUANAN PHARMA
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