Establishing method of fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and fingerprint spectrum

Abstract

The invention discloses an establishing method of a fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and the fingerprint spectrum. The establishing method of the fingerprint spectrum of the honeysuckle-fructus forsythiae heat-clearing tablets includes the following steps: step 1, preparing a test sample solution of the honeysuckle-fructus forsythiae heat-clearing tablets; step 2, respectively precisely sucking the test sample solution and injecting into liquid chromatographs, and recording a chromatogram; step 3, exporting the honeysuckle-fructus forsythiae heat-clearing tablet fingerprint spectrum obtained in the step 2 from the instrument, and importing into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system; selecting chromatographic peaks existing in chromatograms of different batches of the honeysuckle-fructus forsythiae heat-clearing tablets as common peaks; generating a reference fingerprint spectrum of the honeysuckle-fructus forsythiae heat-clearing tablets by an average value calculation method; and calculating the relative retention time and the relative peak area of each common peak. The honeysuckle-fructus forsythiae heat-clearing tablet fingerprint spectrum established by the method provided by the invention can effectively characterize the quality of the honeysuckle-fructus forsythiae heat-clearing tablets, and is conducive to comprehensive supervisory control of the drug quality. The method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like.

Description

technical field [0001] The invention relates to a method for establishing the fingerprint of Yinqiao Qingre Tablet and the fingerprint of Yinqiao Qingre Tablet established by the method. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic study of the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, excellence and stability of the quality of Chinese medicinal materials and Chinese medicinal preparations. Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation ...

AI Technical Summary

Problems solved by technology

Chlorogenic acid, forsythin and arctiin have been quantitatively analyzed in the current implementation standards of this preparation. However, due to the various medicinal flavors and complex components of Yinqiao Qingre Tablets, it is still difficult to fully reflect the comprehensive quality of the drug by qualitative and quantitative analysis of individual components. Information, in order to more effectively control the quality of Yinqiao Qingre Tablets, the present invention has established a fingerprint of the preparation for the active ingredients of Yinqiao Qingre Tablets

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