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HPLC fingerprint spectrum determination method for cannabis sativa

A determination method and a fingerprint technology, which are applied in the field of cannabis HPLC fingerprint determination, can solve the problems of single quality evaluation method of cannabis, unstable measurement effect, poor spectral fit, etc., and achieve good peak shape, easy identification, repeatability and the like. Good results

Inactive Publication Date: 2019-08-16
HANYI BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the quality evaluation method for cannabis is single, mainly using HPLC-UV to detect the content of cannabis polyphenols in cannabis
The determination method of HPLC fingerprint has been widely used in the determination of Chinese medicinal materials and plants, but due to the reasons mentioned in the previous paragraph, HPLC fingerprint has been used in cannabis because of the problems of poor spectrum fitting and unstable measurement effect, so it is not enough. Systematically and completely evaluate the quality of cannabis

Method used

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  • HPLC fingerprint spectrum determination method for cannabis sativa
  • HPLC fingerprint spectrum determination method for cannabis sativa
  • HPLC fingerprint spectrum determination method for cannabis sativa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] The selection of embodiment 1 test sample processing condition

[0091] plan 1)

[0092] Preparation of the test solution: Take an appropriate amount of cannabis flowers and leaves, pulverize them, pass through a No. 2 sieve, and bake at 120° C. for 90 minutes. Take 0.5 g of the roasted cannabis flowers and leaves powder, weigh them accurately, and place them in a conical flask. Add 35ml of 95% ethanol, ultrasonically extract (power: 400W, frequency: 30kHz) for 10 minutes, let it cool to room temperature, pass through a 0.22μm microporous membrane, and take the subsequent filtrate.

[0093] Scenario 2)

[0094] Preparation of the test solution: Take an appropriate amount of cannabis flowers and leaves, pulverize them, pass through a No. 2 sieve, and bake at 120° C. for 90 minutes. Take 0.5 g of the roasted cannabis flowers and leaves powder, weigh them accurately, and place them in a conical flask. Add 50ml of 95% ethanol, ultrasonically extract (power: 400W, frequenc...

Embodiment 2

[0108] The selection of embodiment 2 high performance liquid chromatography conditions

[0109] (1) Inspection of detection wavelength

[0110] Prepare the test solution according to the method of Example 1 scheme (1).

[0111] The assay method of reference example 1 and the high-performance liquid chromatography condition except detection wavelength, measure under the detection wavelength of 210,220,230,254,280nm respectively, record chromatogram, successively as follows Figure 4-8 shown.

[0112] Depend on Figure 4-8 As can be seen, under the detection wavelength of 210 and 220nm, 8 chromatographic peaks ( Figure 4 The peaks shown (7, 9, 10, 11, 12, 13, 17, 19) are clearly distinguishable, and at other detection wavelengths, some peaks are difficult to distinguish and even many components do not appear. And compared with 220nm, the peak shape is better at the detection wavelength of 210nm, so 210nm is selected as the suitable detection wavelength.

[0113] (2) Invest...

Embodiment 3

[0129] Embodiment 3 Precision experiment

[0130] Prepare the test solution according to the method of Example 1 scheme (1).

[0131] Get the same part of the test solution, continuously repeat 6 injections, adopt the chromatographic conditions selected in Example 2, and investigate the precision. The results are shown in Tables 1 and 2. The common peak retention time RSDs were all less than 0.18%, and the peak area RSDs were all less than 0.95%. The similarity between the 6 repeated injections was all 1.000, and the precision was good.

[0132] Table 1 precision experiment result (retention time)

[0133]

[0134]

[0135] Table 2 precision test results (peak area)

[0136]

[0137]

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Abstract

The invention provides an HPLC fingerprint spectrum determination method for cannabis sativa. The method is characterized by comprising the steps of (1) preparing a test solution: taking the cannabissativa medicinal material, crushing, sieving and baking the cannabis sativa medicinal material, precisely weighing baked cannabis sativa medicinal material powder, adding an extracting agent, carryingout ultrasonic extraction, carrying out standing until the room temperature is reached, passing through a microporous filter membrane, and taking subsequent filtrate; (2) preparing a reference solution: taking cannabidiolic acid, cannabidivarin, cannabigerol, cannabidiol, delta 9-tetrahydrocannabinol, cannabichromene, tetrahydrocannabinol and geranylflavone A reference substances, and adding methanol to dissolve the reference substances so as to prepare the reference substance solution; and (3) performing determination: precisely sucking the reference solution and the test solution into an HPLC, performing determination, and recording a chromatogram.

Description

technical field [0001] The invention belongs to the technical fields of drug detection and HPLC detection, and specifically, the invention provides a method for measuring cannabis HPLC fingerprints. Background technique [0002] Cannabis sativa L., also known as hemp, hemp, thread hemp, flax, wild hemp, is an annual herb of Moraceae. Cannabis contains cannabinoids and their derivatives, flavonoids and their glycosides, alkaloids, coumarins, terpenes and sterols, fatty acids, phenanthrenes and other compounds. Cannabinoids are the main active ingredients of cannabis flowers and leaves, mainly including THC (tetrahydrocannabinol, tetrahydrocannabinol), CBD (cannabidiol, cannabidiol), CBC (cannabichromene, cannabichromene), CBN (cannabinol, cannabinol) ), CBG (cannabigerol, cannabigerol) and its propyl homologues THCV, CBDV, CBCV and CBGV, etc. The separation properties, solubility and extraction conditions of these cannabinoid components and solid components are different. T...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/74G01N30/8686G01N2030/027
Inventor 谭昕张景孙武兴王曙宾邢俊波
Owner HANYI BIO TECH CO LTD
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