HPLC fingerprint spectrum determination method for cannabis sativa
A determination method and a fingerprint technology, which are applied in the field of cannabis HPLC fingerprint determination, can solve the problems of single quality evaluation method of cannabis, unstable measurement effect, poor spectral fit, etc., and achieve good peak shape, easy identification, repeatability and the like. Good results
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[0090] Example 1 Selection of treatment conditions for test products
[0091] plan 1)
[0092] Preparation of the test solution: Take an appropriate amount of hemp flower leaf, crush it, pass through a No. 2 sieve, and bake at 120°C for 90 minutes, take 0.5 g of the roasted hemp flower leaf powder, accurately weigh it, and place it in an Erlenmeyer flask. Add 35ml of 95% ethanol, ultrasonically extract (power: 400W, frequency: 30kHz) for 10 minutes, put it to room temperature, pass through a 0.22μm microporous membrane, and take the additional filtrate to get it.
[0093] Scenario 2)
[0094] Preparation of the test solution: Take an appropriate amount of hemp flower leaf, crush it, pass through a No. 2 sieve, and bake at 120°C for 90 minutes, take 0.5 g of the roasted hemp flower leaf powder, accurately weigh it, and place it in an Erlenmeyer flask. Add 50ml of 95% ethanol, ultrasonically extract (power: 400W, frequency: 30kHz) for 20 minutes, put it to room temperature, pass throug...
Example Embodiment
[0108] Example 2 Selection of high performance liquid chromatography conditions
[0109] (1) Inspection of detection wavelength
[0110] Prepare the test solution according to the method of Example 1 scheme (1).
[0111] Refer to the measurement method of Example 1 and the HPLC conditions other than the detection wavelength. Measure at the detection wavelengths of 210, 220, 230, 254, and 280 nm, respectively, and record the chromatogram as follows: Figure 4-8 Shown.
[0112] by Figure 4-8 It can be seen that at the detection wavelengths of 210 and 220nm, the 8 chromatographic peaks ( Figure 4 The peaks 7, 9, 10, 11, 12, 13, 17, 19) are clearly distinguishable. At other detection wavelengths, some peaks are difficult to distinguish and even many components are not peaked. Compared with 220nm, the peak shape is better at the detection wavelength of 210nm, so 210nm is selected as the appropriate detection wavelength.
[0113] (2) Mobile phase investigation
[0114] Prepare the test solu...
Example Embodiment
[0129] Example 3 Precision Experiment
[0130] Prepare the test solution according to the method of Example 1 scheme (1).
[0131] Take the same test solution, repeat 6 injections continuously, and use the chromatographic conditions selected in Example 2 to investigate the precision. The results are shown in Tables 1 and 2. The shared peak retention time RSD is less than 0.18%, the peak area RSD is not greater than 0.95%, and the similarity between the six injections is 1.000, and the precision is good.
[0132] Table 1 Precision test results (retention time)
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[0135] Table 2 Precision test results (peak area)
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